Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1859 and Psest_1860 are separated by 87 nucleotides Psest_1860 and Psest_1861 are separated by 34 nucleotides Psest_1861 and Psest_1862 are separated by 85 nucleotides
Psest_1859: Psest_1859 - Glutathione S-transferase, at 1,963,564 to 1,964,235
_1859
Psest_1860: Psest_1860 - Uncharacterized conserved protein, at 1,964,323 to 1,964,910
_1860
Psest_1861: Psest_1861 - type I secretion membrane fusion protein, HlyD family, at 1,964,945 to 1,966,297
_1861
Psest_1862: Psest_1862 - VCBS repeat/type 1 secretion C-terminal target domain (VC_A0849 subclass), at 1,966,383 to 1,973,558
_1862
Position (kb)
1964
1965
1966
1967 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1964.159 kb on + strand, within Psest_1859 at 1964.159 kb on + strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.250 kb on - strand at 1964.268 kb on + strand at 1964.545 kb on + strand, within Psest_1860 at 1964.545 kb on + strand, within Psest_1860 at 1964.546 kb on - strand, within Psest_1860 at 1964.664 kb on + strand, within Psest_1860 at 1964.881 kb on + strand at 1964.946 kb on + strand at 1964.947 kb on - strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1965.118 kb on - strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.125 kb on - strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.199 kb on - strand, within Psest_1861 at 1965.267 kb on - strand, within Psest_1861 at 1965.333 kb on - strand, within Psest_1861 at 1965.333 kb on - strand, within Psest_1861 at 1965.381 kb on - strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.457 kb on + strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.458 kb on - strand, within Psest_1861 at 1965.600 kb on - strand, within Psest_1861 at 1965.651 kb on - strand, within Psest_1861 at 1965.686 kb on - strand, within Psest_1861 at 1965.706 kb on - strand, within Psest_1861 at 1965.706 kb on - strand, within Psest_1861 at 1965.811 kb on - strand, within Psest_1861 at 1965.811 kb on - strand, within Psest_1861 at 1966.026 kb on + strand, within Psest_1861 at 1966.027 kb on - strand, within Psest_1861 at 1966.027 kb on - strand, within Psest_1861 at 1966.129 kb on - strand, within Psest_1861 at 1966.129 kb on - strand, within Psest_1861 at 1966.158 kb on - strand, within Psest_1861 at 1966.236 kb on + strand at 1966.237 kb on - strand at 1966.343 kb on + strand at 1966.496 kb on + strand at 1966.771 kb on + strand at 1966.771 kb on + strand at 1966.771 kb on + strand at 1966.771 kb on + strand at 1966.837 kb on + strand at 1966.837 kb on + strand at 1966.878 kb on - strand at 1966.953 kb on + strand at 1966.983 kb on - strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.986 kb on + strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.987 kb on - strand at 1966.988 kb on + strand at 1966.988 kb on + strand at 1966.988 kb on + strand at 1966.988 kb on + strand at 1966.988 kb on + strand at 1966.988 kb on + strand at 1966.988 kb on + strand at 1966.989 kb on - strand at 1966.989 kb on - strand at 1966.992 kb on + strand at 1966.992 kb on + strand at 1966.992 kb on + strand at 1966.992 kb on + strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1966.993 kb on - strand at 1967.064 kb on + strand at 1967.065 kb on - strand at 1967.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 1,964,159 + Psest_1859 0.89 -2.5 1,964,159 + Psest_1859 0.89 -0.9 1,964,160 - Psest_1859 0.89 +1.3 1,964,160 - Psest_1859 0.89 +0.1 1,964,160 - Psest_1859 0.89 -0.4 1,964,250 - +1.7 1,964,268 + +0.2 1,964,545 + Psest_1860 0.38 +0.6 1,964,545 + Psest_1860 0.38 -0.1 1,964,546 - Psest_1860 0.38 +0.6 1,964,664 + Psest_1860 0.58 -1.1 1,964,881 + -1.1 1,964,946 + +1.6 1,964,947 - -2.4 1,964,997 + -0.1 1,964,997 + +0.2 1,964,997 + -0.4 1,964,997 + +1.1 1,964,998 - +1.9 1,964,998 - -2.0 1,964,998 - -0.6 1,964,998 - +0.0 1,965,118 - Psest_1861 0.13 +0.7 1,965,124 + Psest_1861 0.13 -1.9 1,965,124 + Psest_1861 0.13 +1.0 1,965,124 + Psest_1861 0.13 +2.0 1,965,125 - Psest_1861 0.13 +0.5 1,965,198 + Psest_1861 0.19 -1.3 1,965,198 + Psest_1861 0.19 +2.5 1,965,198 + Psest_1861 0.19 -0.5 1,965,199 - Psest_1861 0.19 -1.8 1,965,267 - Psest_1861 0.24 +0.7 1,965,333 - Psest_1861 0.29 +2.1 1,965,333 - Psest_1861 0.29 +0.4 1,965,381 - Psest_1861 0.32 +0.3 1,965,457 + Psest_1861 0.38 +0.9 1,965,457 + Psest_1861 0.38 +3.4 1,965,457 + Psest_1861 0.38 +1.1 1,965,457 + Psest_1861 0.38 -0.2 1,965,458 - Psest_1861 0.38 +2.2 1,965,458 - Psest_1861 0.38 +3.1 1,965,458 - Psest_1861 0.38 +1.2 1,965,458 - Psest_1861 0.38 -1.8 1,965,600 - Psest_1861 0.48 +0.4 1,965,651 - Psest_1861 0.52 -0.5 1,965,686 - Psest_1861 0.55 -1.4 1,965,706 - Psest_1861 0.56 -0.9 1,965,706 - Psest_1861 0.56 +1.3 1,965,811 - Psest_1861 0.64 -0.4 1,965,811 - Psest_1861 0.64 +1.1 1,966,026 + Psest_1861 0.80 -0.6 1,966,027 - Psest_1861 0.80 +0.9 1,966,027 - Psest_1861 0.80 -0.6 1,966,129 - Psest_1861 0.88 -0.6 1,966,129 - Psest_1861 0.88 -0.6 1,966,158 - Psest_1861 0.90 +1.1 1,966,236 + -0.2 1,966,237 - -1.8 1,966,343 + -0.8 1,966,496 + +0.4 1,966,771 + +0.9 1,966,771 + -1.8 1,966,771 + -3.5 1,966,771 + -2.3 1,966,837 + -0.6 1,966,837 + -0.4 1,966,878 - -1.4 1,966,953 + -2.9 1,966,983 - -1.1 1,966,986 + +0.2 1,966,986 + +2.3 1,966,986 + -1.8 1,966,986 + -1.9 1,966,986 + +1.7 1,966,986 + +0.3 1,966,986 + +2.3 1,966,986 + +1.7 1,966,986 + +0.7 1,966,986 + -1.1 1,966,986 + +0.3 1,966,986 + -2.5 1,966,986 + +0.2 1,966,986 + +0.4 1,966,986 + -2.4 1,966,986 + +0.2 1,966,986 + +0.5 1,966,986 + -0.1 1,966,987 - -2.7 1,966,987 - +0.5 1,966,987 - -1.0 1,966,987 - +0.0 1,966,987 - -3.6 1,966,987 - +0.9 1,966,987 - +4.7 1,966,987 - -0.6 1,966,987 - +1.7 1,966,987 - +1.9 1,966,987 - +0.4 1,966,987 - +0.6 1,966,987 - -0.1 1,966,988 + -1.1 1,966,988 + +0.2 1,966,988 + +1.2 1,966,988 + +0.9 1,966,988 + +1.9 1,966,988 + -3.0 1,966,988 + +0.7 1,966,989 - -2.9 1,966,989 - -0.6 1,966,992 + +1.4 1,966,992 + +2.9 1,966,992 + +1.7 1,966,992 + +0.5 1,966,993 - +0.4 1,966,993 - -0.7 1,966,993 - +1.1 1,966,993 - +0.8 1,966,993 - -0.7 1,966,993 - -0.3 1,966,993 - -0.1 1,966,993 - +0.7 1,966,993 - +0.6 1,966,993 - -2.4 1,966,993 - +1.2 1,966,993 - +0.2 1,967,064 + -3.3 1,967,065 - -0.3 1,967,065 - +1.8
Or see this region's nucleotide sequence