Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1358 and Psest_1359 are separated by 134 nucleotides Psest_1359 and Psest_1360 are separated by 108 nucleotides Psest_1360 and Psest_1361 overlap by 4 nucleotides Psest_1361 and Psest_1362 overlap by 8 nucleotides
Psest_1358: Psest_1358 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 1,468,242 to 1,469,489
_1358
Psest_1359: Psest_1359 - Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases, at 1,469,624 to 1,470,067
_1359
Psest_1360: Psest_1360 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,470,176 to 1,471,153
_1360
Psest_1361: Psest_1361 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 1,471,150 to 1,471,911
_1361
Psest_1362: Psest_1362 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 1,471,904 to 1,472,548
_1362
Position (kb)
1470
1471
1472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1469.362 kb on + strand, within Psest_1358 at 1469.362 kb on + strand, within Psest_1358 at 1469.363 kb on - strand, within Psest_1358 at 1469.363 kb on - strand, within Psest_1358 at 1469.363 kb on - strand, within Psest_1358 at 1469.363 kb on - strand, within Psest_1358 at 1469.363 kb on - strand, within Psest_1358 at 1469.589 kb on + strand at 1469.590 kb on - strand at 1469.642 kb on + strand at 1469.642 kb on + strand at 1469.642 kb on + strand at 1469.694 kb on - strand, within Psest_1359 at 1469.718 kb on + strand, within Psest_1359 at 1469.718 kb on + strand, within Psest_1359 at 1469.719 kb on - strand, within Psest_1359 at 1469.719 kb on - strand, within Psest_1359 at 1469.719 kb on - strand, within Psest_1359 at 1469.719 kb on - strand, within Psest_1359 at 1469.719 kb on - strand, within Psest_1359 at 1469.719 kb on - strand, within Psest_1359 at 1470.136 kb on + strand at 1470.136 kb on + strand at 1470.137 kb on - strand at 1470.137 kb on - strand at 1470.137 kb on - strand at 1470.165 kb on - strand at 1470.336 kb on + strand, within Psest_1360 at 1470.336 kb on + strand, within Psest_1360 at 1470.336 kb on + strand, within Psest_1360 at 1470.337 kb on - strand, within Psest_1360 at 1470.337 kb on - strand, within Psest_1360 at 1470.337 kb on - strand, within Psest_1360 at 1470.769 kb on + strand, within Psest_1360 at 1471.474 kb on + strand, within Psest_1361 at 1471.474 kb on + strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.764 kb on + strand, within Psest_1361 at 1471.764 kb on + strand, within Psest_1361 at 1471.764 kb on + strand, within Psest_1361 at 1471.804 kb on + strand, within Psest_1361
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 1,469,362 + Psest_1358 0.90 +2.7 1,469,362 + Psest_1358 0.90 +0.1 1,469,363 - Psest_1358 0.90 +0.9 1,469,363 - Psest_1358 0.90 -1.6 1,469,363 - Psest_1358 0.90 -0.7 1,469,363 - Psest_1358 0.90 -0.4 1,469,363 - Psest_1358 0.90 -0.0 1,469,589 + +1.5 1,469,590 - -0.5 1,469,642 + +3.1 1,469,642 + -0.1 1,469,642 + +2.3 1,469,694 - Psest_1359 0.16 +1.3 1,469,718 + Psest_1359 0.21 -0.1 1,469,718 + Psest_1359 0.21 +2.0 1,469,719 - Psest_1359 0.21 +2.2 1,469,719 - Psest_1359 0.21 +1.8 1,469,719 - Psest_1359 0.21 -0.4 1,469,719 - Psest_1359 0.21 -0.2 1,469,719 - Psest_1359 0.21 -0.2 1,469,719 - Psest_1359 0.21 -1.3 1,470,136 + -1.8 1,470,136 + +1.3 1,470,137 - +1.9 1,470,137 - -1.0 1,470,137 - -3.4 1,470,165 - -0.6 1,470,336 + Psest_1360 0.16 +4.2 1,470,336 + Psest_1360 0.16 -2.1 1,470,336 + Psest_1360 0.16 +2.4 1,470,337 - Psest_1360 0.16 -2.8 1,470,337 - Psest_1360 0.16 -1.1 1,470,337 - Psest_1360 0.16 -1.5 1,470,769 + Psest_1360 0.61 +0.7 1,471,474 + Psest_1361 0.43 +2.5 1,471,474 + Psest_1361 0.43 +0.1 1,471,475 - Psest_1361 0.43 +1.7 1,471,475 - Psest_1361 0.43 -0.3 1,471,475 - Psest_1361 0.43 -2.4 1,471,475 - Psest_1361 0.43 +0.2 1,471,475 - Psest_1361 0.43 -0.6 1,471,475 - Psest_1361 0.43 -0.4 1,471,764 + Psest_1361 0.81 -0.1 1,471,764 + Psest_1361 0.81 -0.7 1,471,764 + Psest_1361 0.81 +3.3 1,471,804 + Psest_1361 0.86 +0.2
Or see this region's nucleotide sequence