Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1339 and Psest_1340 are separated by 38 nucleotides Psest_1340 and Psest_1341 are separated by 37 nucleotides Psest_1341 and Psest_1342 are separated by 60 nucleotides
Psest_1339: Psest_1339 - ATP-dependent helicase HrpA, at 1,446,570 to 1,450,652
_1339
Psest_1340: Psest_1340 - rarD protein, at 1,450,691 to 1,451,620
_1340
Psest_1341: Psest_1341 - FOG: FHA domain, at 1,451,658 to 1,452,512
_1341
Psest_1342: Psest_1342 - Protein kinase domain., at 1,452,573 to 1,454,126
_1342
Position (kb)
1450
1451
1452 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1450.374 kb on + strand at 1450.540 kb on - strand at 1450.561 kb on - strand at 1450.622 kb on - strand at 1450.760 kb on + strand at 1450.760 kb on + strand at 1450.760 kb on + strand at 1450.761 kb on - strand at 1450.761 kb on - strand at 1450.761 kb on - strand at 1450.761 kb on - strand at 1450.761 kb on - strand at 1450.761 kb on - strand at 1450.763 kb on + strand at 1450.763 kb on + strand at 1450.763 kb on + strand at 1450.763 kb on + strand at 1450.763 kb on + strand at 1450.763 kb on + strand at 1450.763 kb on + strand at 1450.764 kb on - strand at 1450.764 kb on - strand at 1450.764 kb on - strand at 1450.764 kb on - strand at 1450.766 kb on + strand at 1450.766 kb on + strand at 1450.766 kb on + strand at 1450.766 kb on + strand at 1450.766 kb on + strand at 1450.766 kb on + strand at 1450.766 kb on + strand at 1450.766 kb on + strand at 1450.767 kb on - strand at 1450.767 kb on - strand at 1450.767 kb on - strand at 1450.806 kb on - strand, within Psest_1340 at 1451.043 kb on + strand, within Psest_1340 at 1451.043 kb on + strand, within Psest_1340 at 1451.192 kb on + strand, within Psest_1340 at 1451.193 kb on - strand, within Psest_1340 at 1451.193 kb on - strand, within Psest_1340 at 1451.435 kb on + strand, within Psest_1340 at 1451.435 kb on + strand, within Psest_1340 at 1451.435 kb on + strand, within Psest_1340 at 1451.436 kb on - strand, within Psest_1340 at 1451.436 kb on - strand, within Psest_1340 at 1451.436 kb on - strand, within Psest_1340 at 1451.436 kb on - strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.510 kb on + strand, within Psest_1340 at 1451.511 kb on - strand, within Psest_1340 at 1451.511 kb on - strand, within Psest_1340 at 1451.511 kb on - strand, within Psest_1340 at 1451.511 kb on - strand, within Psest_1340 at 1451.511 kb on - strand, within Psest_1340 at 1451.952 kb on + strand, within Psest_1341 at 1451.952 kb on + strand, within Psest_1341 at 1451.952 kb on + strand, within Psest_1341 at 1451.952 kb on + strand, within Psest_1341 at 1451.953 kb on - strand, within Psest_1341 at 1451.953 kb on - strand, within Psest_1341 at 1452.058 kb on + strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.059 kb on - strand, within Psest_1341 at 1452.151 kb on + strand, within Psest_1341 at 1452.151 kb on + strand, within Psest_1341 at 1452.151 kb on + strand, within Psest_1341 at 1452.152 kb on - strand, within Psest_1341 at 1452.152 kb on - strand, within Psest_1341 at 1452.322 kb on + strand, within Psest_1341 at 1452.322 kb on + strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.323 kb on - strand, within Psest_1341 at 1452.355 kb on + strand, within Psest_1341 at 1452.356 kb on - strand, within Psest_1341 at 1452.356 kb on - strand, within Psest_1341 at 1452.358 kb on + strand, within Psest_1341 at 1452.359 kb on - strand, within Psest_1341 at 1452.359 kb on - strand, within Psest_1341 at 1452.521 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 1,450,374 + -2.0 1,450,540 - -0.0 1,450,561 - -0.5 1,450,622 - -2.2 1,450,760 + -0.1 1,450,760 + -2.2 1,450,760 + +0.3 1,450,761 - -0.5 1,450,761 - -0.4 1,450,761 - -0.6 1,450,761 - -2.5 1,450,761 - +0.2 1,450,761 - -2.0 1,450,763 + -1.0 1,450,763 + -2.1 1,450,763 + +1.0 1,450,763 + +0.6 1,450,763 + +1.3 1,450,763 + -0.2 1,450,763 + +0.8 1,450,764 - -1.3 1,450,764 - -0.6 1,450,764 - +1.0 1,450,764 - -0.3 1,450,766 + +1.1 1,450,766 + +0.8 1,450,766 + +1.9 1,450,766 + +0.6 1,450,766 + +1.0 1,450,766 + +1.5 1,450,766 + +2.7 1,450,766 + +2.1 1,450,767 - +0.6 1,450,767 - -1.2 1,450,767 - -1.3 1,450,806 - Psest_1340 0.12 -3.9 1,451,043 + Psest_1340 0.38 +1.3 1,451,043 + Psest_1340 0.38 -1.8 1,451,192 + Psest_1340 0.54 -0.4 1,451,193 - Psest_1340 0.54 -1.8 1,451,193 - Psest_1340 0.54 +1.3 1,451,435 + Psest_1340 0.80 -1.0 1,451,435 + Psest_1340 0.80 +1.0 1,451,435 + Psest_1340 0.80 +1.1 1,451,436 - Psest_1340 0.80 +0.5 1,451,436 - Psest_1340 0.80 -2.2 1,451,436 - Psest_1340 0.80 +1.9 1,451,436 - Psest_1340 0.80 -1.4 1,451,510 + Psest_1340 0.88 -1.1 1,451,510 + Psest_1340 0.88 -2.2 1,451,510 + Psest_1340 0.88 +1.7 1,451,510 + Psest_1340 0.88 +1.0 1,451,510 + Psest_1340 0.88 +0.3 1,451,510 + Psest_1340 0.88 -1.8 1,451,510 + Psest_1340 0.88 +0.8 1,451,510 + Psest_1340 0.88 +0.3 1,451,510 + Psest_1340 0.88 +0.2 1,451,510 + Psest_1340 0.88 +4.1 1,451,511 - Psest_1340 0.88 -1.8 1,451,511 - Psest_1340 0.88 +4.8 1,451,511 - Psest_1340 0.88 -0.8 1,451,511 - Psest_1340 0.88 +1.8 1,451,511 - Psest_1340 0.88 -0.7 1,451,952 + Psest_1341 0.34 -0.6 1,451,952 + Psest_1341 0.34 -1.6 1,451,952 + Psest_1341 0.34 -0.9 1,451,952 + Psest_1341 0.34 +2.9 1,451,953 - Psest_1341 0.35 -2.9 1,451,953 - Psest_1341 0.35 -0.6 1,452,058 + Psest_1341 0.47 +1.5 1,452,059 - Psest_1341 0.47 -1.2 1,452,059 - Psest_1341 0.47 -0.0 1,452,059 - Psest_1341 0.47 -1.4 1,452,059 - Psest_1341 0.47 -0.6 1,452,059 - Psest_1341 0.47 -1.7 1,452,059 - Psest_1341 0.47 +1.4 1,452,059 - Psest_1341 0.47 +2.1 1,452,059 - Psest_1341 0.47 -0.6 1,452,151 + Psest_1341 0.58 -2.2 1,452,151 + Psest_1341 0.58 -0.0 1,452,151 + Psest_1341 0.58 -1.8 1,452,152 - Psest_1341 0.58 -1.3 1,452,152 - Psest_1341 0.58 +1.1 1,452,322 + Psest_1341 0.78 -1.7 1,452,322 + Psest_1341 0.78 +2.4 1,452,323 - Psest_1341 0.78 -2.1 1,452,323 - Psest_1341 0.78 +3.2 1,452,323 - Psest_1341 0.78 -0.8 1,452,323 - Psest_1341 0.78 -1.9 1,452,323 - Psest_1341 0.78 +1.3 1,452,355 + Psest_1341 0.82 -2.7 1,452,356 - Psest_1341 0.82 +0.3 1,452,356 - Psest_1341 0.82 -1.9 1,452,358 + Psest_1341 0.82 -1.4 1,452,359 - Psest_1341 0.82 -0.0 1,452,359 - Psest_1341 0.82 +1.0 1,452,521 + -0.5
Or see this region's nucleotide sequence