Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0995 and Psest_0996 overlap by 4 nucleotides Psest_0996 and Psest_0997 are separated by 123 nucleotides Psest_0997 and Psest_0998 are separated by 1 nucleotides
Psest_0995: Psest_0995 - pantoate--beta-alanine ligase, at 1,078,150 to 1,079,010
_0995
Psest_0996: Psest_0996 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,079,007 to 1,079,807
_0996
Psest_0997: Psest_0997 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, at 1,079,931 to 1,080,458
_0997
Psest_0998: Psest_0998 - poly(A) polymerase, at 1,080,460 to 1,081,854
_0998
Position (kb)
1079
1080
1081 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.943 kb on + strand at 1079.944 kb on - strand at 1079.944 kb on - strand at 1081.359 kb on - strand, within Psest_0998 at 1081.362 kb on - strand, within Psest_0998
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 1,079,895 - -0.7 1,079,895 - -0.5 1,079,895 - +0.3 1,079,895 - -2.3 1,079,895 - -0.7 1,079,915 - -0.4 1,079,915 - +2.6 1,079,915 - +0.9 1,079,915 - +3.2 1,079,915 - +0.5 1,079,915 - -0.2 1,079,915 - -1.2 1,079,943 + +1.0 1,079,944 - -1.6 1,079,944 - +1.0 1,081,359 - Psest_0998 0.64 -1.2 1,081,362 - Psest_0998 0.65 +0.9
Or see this region's nucleotide sequence