Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0993 and Psest_0994 are separated by 356 nucleotides Psest_0994 and Psest_0995 are separated by 83 nucleotides Psest_0995 and Psest_0996 overlap by 4 nucleotides
Psest_0993: Psest_0993 - Glucose-6-phosphate isomerase, at 1,075,665 to 1,077,329
_0993
Psest_0994: Psest_0994 - L-aspartate-alpha-decarboxylase, at 1,077,686 to 1,078,066
_0994
Psest_0995: Psest_0995 - pantoate--beta-alanine ligase, at 1,078,150 to 1,079,010
_0995
Psest_0996: Psest_0996 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,079,007 to 1,079,807
_0996
Position (kb)
1077
1078
1079 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1076.779 kb on + strand, within Psest_0993 at 1076.779 kb on + strand, within Psest_0993 at 1076.779 kb on + strand, within Psest_0993 at 1076.780 kb on - strand, within Psest_0993 at 1076.780 kb on - strand, within Psest_0993 at 1076.780 kb on - strand, within Psest_0993 at 1076.780 kb on - strand, within Psest_0993 at 1076.814 kb on + strand, within Psest_0993 at 1077.319 kb on + strand at 1077.319 kb on + strand at 1077.319 kb on + strand at 1077.322 kb on + strand at 1077.322 kb on + strand at 1077.322 kb on + strand at 1077.322 kb on + strand at 1077.322 kb on + strand at 1077.322 kb on + strand at 1077.323 kb on - strand at 1077.323 kb on - strand at 1077.323 kb on - strand at 1077.323 kb on - strand at 1077.323 kb on - strand at 1077.323 kb on - strand at 1077.323 kb on - strand at 1077.323 kb on - strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.415 kb on + strand at 1077.416 kb on - strand at 1077.416 kb on - strand at 1077.416 kb on - strand at 1077.416 kb on - strand at 1077.416 kb on - strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.419 kb on + strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.420 kb on - strand at 1077.507 kb on + strand at 1077.507 kb on + strand at 1077.507 kb on + strand at 1077.507 kb on + strand at 1077.507 kb on + strand at 1077.507 kb on + strand at 1077.567 kb on + strand at 1077.567 kb on + strand at 1077.567 kb on + strand at 1077.567 kb on + strand at 1077.747 kb on + strand, within Psest_0994 at 1077.747 kb on + strand, within Psest_0994 at 1077.747 kb on + strand, within Psest_0994 at 1077.747 kb on + strand, within Psest_0994 at 1077.747 kb on + strand, within Psest_0994 at 1077.747 kb on + strand, within Psest_0994 at 1077.747 kb on + strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.748 kb on - strand, within Psest_0994 at 1077.789 kb on + strand, within Psest_0994 at 1077.789 kb on + strand, within Psest_0994 at 1077.790 kb on - strand, within Psest_0994 at 1077.790 kb on - strand, within Psest_0994 at 1077.895 kb on - strand, within Psest_0994 at 1077.895 kb on - strand, within Psest_0994 at 1077.895 kb on - strand, within Psest_0994 at 1077.895 kb on - strand, within Psest_0994 at 1077.926 kb on - strand, within Psest_0994 at 1077.927 kb on + strand, within Psest_0994 at 1077.927 kb on + strand, within Psest_0994 at 1077.927 kb on + strand, within Psest_0994 at 1077.927 kb on + strand, within Psest_0994 at 1077.927 kb on + strand, within Psest_0994 at 1077.927 kb on + strand, within Psest_0994 at 1077.927 kb on + strand, within Psest_0994 at 1077.928 kb on - strand, within Psest_0994 at 1077.928 kb on - strand, within Psest_0994 at 1077.928 kb on - strand, within Psest_0994 at 1077.928 kb on - strand, within Psest_0994 at 1077.928 kb on - strand, within Psest_0994 at 1077.928 kb on - strand, within Psest_0994 at 1077.945 kb on + strand, within Psest_0994 at 1077.946 kb on - strand, within Psest_0994 at 1077.946 kb on - strand, within Psest_0994
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 1,076,779 + Psest_0993 0.67 +0.1 1,076,779 + Psest_0993 0.67 +0.6 1,076,779 + Psest_0993 0.67 -1.1 1,076,780 - Psest_0993 0.67 +0.1 1,076,780 - Psest_0993 0.67 -1.8 1,076,780 - Psest_0993 0.67 -0.0 1,076,780 - Psest_0993 0.67 -2.4 1,076,814 + Psest_0993 0.69 +0.2 1,077,319 + -1.8 1,077,319 + +0.9 1,077,319 + -0.3 1,077,322 + -0.4 1,077,322 + -2.1 1,077,322 + +2.2 1,077,322 + +2.4 1,077,322 + -2.0 1,077,322 + -0.3 1,077,323 - +1.2 1,077,323 - -0.9 1,077,323 - -1.9 1,077,323 - -0.6 1,077,323 - -2.8 1,077,323 - +0.9 1,077,323 - -0.5 1,077,323 - -3.5 1,077,415 + +0.1 1,077,415 + -0.3 1,077,415 + -2.2 1,077,415 + -1.9 1,077,415 + +0.8 1,077,415 + +2.9 1,077,415 + -0.6 1,077,415 + -3.1 1,077,415 + -1.8 1,077,415 + -0.4 1,077,415 + -2.8 1,077,415 + +0.5 1,077,415 + -2.7 1,077,415 + +1.6 1,077,415 + +1.0 1,077,416 - +0.3 1,077,416 - -2.1 1,077,416 - -1.7 1,077,416 - +0.5 1,077,416 - -1.2 1,077,419 + -0.3 1,077,419 + -0.5 1,077,419 + -3.3 1,077,419 + -0.9 1,077,419 + -0.4 1,077,419 + -0.9 1,077,419 + -0.6 1,077,419 + +3.7 1,077,419 + +1.2 1,077,419 + -0.2 1,077,419 + -0.1 1,077,419 + -2.8 1,077,419 + -0.1 1,077,419 + -1.1 1,077,419 + +0.8 1,077,419 + -0.1 1,077,419 + +1.2 1,077,419 + -1.8 1,077,419 + -1.5 1,077,419 + +0.2 1,077,419 + +0.8 1,077,419 + +1.3 1,077,419 + -0.8 1,077,419 + +1.0 1,077,419 + -2.0 1,077,419 + +1.1 1,077,419 + +1.6 1,077,419 + +0.6 1,077,419 + -2.6 1,077,419 + +3.4 1,077,419 + -1.0 1,077,419 + -2.1 1,077,419 + -1.1 1,077,419 + -0.7 1,077,419 + -2.5 1,077,419 + -4.0 1,077,419 + +0.9 1,077,419 + -0.6 1,077,419 + -3.3 1,077,419 + -0.7 1,077,419 + +2.8 1,077,419 + -1.5 1,077,419 + +0.9 1,077,419 + -1.4 1,077,419 + -0.7 1,077,420 - -1.2 1,077,420 - +0.1 1,077,420 - -2.0 1,077,420 - -1.3 1,077,420 - -2.3 1,077,420 - -1.4 1,077,420 - -0.6 1,077,420 - -1.5 1,077,420 - -0.2 1,077,420 - -2.0 1,077,420 - -2.4 1,077,420 - -0.2 1,077,420 - +1.8 1,077,420 - -0.6 1,077,420 - -1.3 1,077,507 + -0.3 1,077,507 + -1.0 1,077,507 + +0.9 1,077,507 + +1.5 1,077,507 + +0.3 1,077,507 + +0.9 1,077,567 + +1.4 1,077,567 + +1.5 1,077,567 + +1.8 1,077,567 + -2.4 1,077,747 + Psest_0994 0.16 -0.3 1,077,747 + Psest_0994 0.16 +0.3 1,077,747 + Psest_0994 0.16 +0.5 1,077,747 + Psest_0994 0.16 -1.4 1,077,747 + Psest_0994 0.16 +1.4 1,077,747 + Psest_0994 0.16 -0.3 1,077,747 + Psest_0994 0.16 -2.2 1,077,748 - Psest_0994 0.16 +1.3 1,077,748 - Psest_0994 0.16 -0.4 1,077,748 - Psest_0994 0.16 +0.7 1,077,748 - Psest_0994 0.16 -0.3 1,077,748 - Psest_0994 0.16 -0.8 1,077,748 - Psest_0994 0.16 +0.2 1,077,748 - Psest_0994 0.16 -0.4 1,077,748 - Psest_0994 0.16 -0.1 1,077,789 + Psest_0994 0.27 -0.3 1,077,789 + Psest_0994 0.27 +1.6 1,077,790 - Psest_0994 0.27 -0.7 1,077,790 - Psest_0994 0.27 -1.9 1,077,895 - Psest_0994 0.55 +0.6 1,077,895 - Psest_0994 0.55 -1.1 1,077,895 - Psest_0994 0.55 -2.0 1,077,895 - Psest_0994 0.55 -0.1 1,077,926 - Psest_0994 0.63 -2.4 1,077,927 + Psest_0994 0.63 -2.2 1,077,927 + Psest_0994 0.63 +1.5 1,077,927 + Psest_0994 0.63 -1.2 1,077,927 + Psest_0994 0.63 -2.0 1,077,927 + Psest_0994 0.63 -1.1 1,077,927 + Psest_0994 0.63 -2.1 1,077,927 + Psest_0994 0.63 -1.5 1,077,928 - Psest_0994 0.64 -0.0 1,077,928 - Psest_0994 0.64 -1.9 1,077,928 - Psest_0994 0.64 +0.0 1,077,928 - Psest_0994 0.64 -0.4 1,077,928 - Psest_0994 0.64 -2.7 1,077,928 - Psest_0994 0.64 -1.2 1,077,945 + Psest_0994 0.68 +0.5 1,077,946 - Psest_0994 0.68 -1.3 1,077,946 - Psest_0994 0.68 -2.2
Or see this region's nucleotide sequence