Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0968 and Psest_0969 overlap by 4 nucleotides Psest_0969 and Psest_0970 overlap by 23 nucleotides Psest_0970 and Psest_0971 are separated by 35 nucleotides Psest_0971 and Psest_0972 are separated by 532 nucleotides
Psest_0968: Psest_0968 - carbamoyl-phosphate synthase, large subunit, at 1,049,806 to 1,053,027
_0968
Psest_0969: Psest_0969 - transcription elongation factor GreA, at 1,053,024 to 1,053,500
_0969
Psest_0970: Psest_0970 - hypothetical protein, at 1,053,478 to 1,053,909
_0970
Psest_0971: Psest_0971 - Predicted RNA-binding protein containing KH domain, possibly ribosomal protein, at 1,053,945 to 1,054,256
_0971
Psest_0972: Psest_0972 - ATP-dependent metalloprotease FtsH, at 1,054,789 to 1,056,708
_0972
Position (kb)
1053
1054 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1053.302 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.336 kb on + strand, within Psest_0969 at 1053.494 kb on + strand at 1053.495 kb on - strand at 1053.495 kb on - strand at 1053.844 kb on + strand, within Psest_0970 at 1053.844 kb on + strand, within Psest_0970 at 1053.844 kb on + strand, within Psest_0970 at 1053.844 kb on + strand, within Psest_0970 at 1053.845 kb on - strand, within Psest_0970 at 1054.226 kb on - strand at 1054.257 kb on - strand at 1054.785 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 1,053,302 + Psest_0969 0.58 -0.6 1,053,336 + Psest_0969 0.65 -0.9 1,053,336 + Psest_0969 0.65 -0.6 1,053,336 + Psest_0969 0.65 +0.4 1,053,336 + Psest_0969 0.65 -0.1 1,053,494 + -1.6 1,053,495 - -0.3 1,053,495 - +0.6 1,053,844 + Psest_0970 0.85 -0.9 1,053,844 + Psest_0970 0.85 -0.5 1,053,844 + Psest_0970 0.85 -1.3 1,053,844 + Psest_0970 0.85 +0.2 1,053,845 - Psest_0970 0.85 -2.1 1,054,226 - -1.8 1,054,257 - +2.0 1,054,785 + +1.3
Or see this region's nucleotide sequence