Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0918 and Psest_0919 are separated by 112 nucleotides Psest_0919 and Psest_0920 are separated by 137 nucleotides Psest_0920 and Psest_0921 are separated by 76 nucleotides
Psest_0918: Psest_0918 - Predicted permease, DMT superfamily, at 998,650 to 999,558
_0918
Psest_0919: Psest_0919 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 999,671 to 1,000,627
_0919
Psest_0920: Psest_0920 - Transcriptional regulators, at 1,000,765 to 1,001,250
_0920
Psest_0921: Psest_0921 - AraC-type DNA-binding domain-containing proteins, at 1,001,327 to 1,002,154
_0921
Position (kb) 999
1000
1001 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.672 kb on + strand at 998.673 kb on - strand at 998.756 kb on + strand, within Psest_0918 at 998.756 kb on + strand, within Psest_0918 at 998.756 kb on + strand, within Psest_0918 at 998.756 kb on + strand, within Psest_0918 at 998.767 kb on + strand, within Psest_0918 at 998.861 kb on + strand, within Psest_0918 at 998.861 kb on + strand, within Psest_0918 at 998.862 kb on - strand, within Psest_0918 at 998.862 kb on - strand, within Psest_0918 at 998.862 kb on - strand, within Psest_0918 at 998.867 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.954 kb on + strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 998.955 kb on - strand, within Psest_0918 at 999.047 kb on - strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.127 kb on + strand, within Psest_0918 at 999.128 kb on - strand, within Psest_0918 at 999.128 kb on - strand, within Psest_0918 at 999.128 kb on - strand, within Psest_0918 at 999.156 kb on + strand, within Psest_0918 at 999.156 kb on + strand, within Psest_0918 at 999.156 kb on + strand, within Psest_0918 at 999.157 kb on - strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.160 kb on + strand, within Psest_0918 at 999.161 kb on - strand, within Psest_0918 at 999.196 kb on - strand, within Psest_0918 at 999.237 kb on + strand, within Psest_0918 at 999.316 kb on + strand, within Psest_0918 at 999.317 kb on - strand, within Psest_0918 at 999.362 kb on + strand, within Psest_0918 at 999.373 kb on + strand, within Psest_0918 at 999.374 kb on - strand, within Psest_0918 at 999.374 kb on - strand, within Psest_0918 at 999.497 kb on + strand at 999.498 kb on - strand at 999.498 kb on - strand at 999.498 kb on - strand at 999.561 kb on - strand at 999.652 kb on + strand at 999.652 kb on + strand at 999.652 kb on + strand at 999.652 kb on + strand at 999.653 kb on - strand at 999.653 kb on - strand at 999.653 kb on - strand at 999.653 kb on - strand at 999.654 kb on + strand at 999.654 kb on + strand at 999.654 kb on + strand at 999.654 kb on + strand at 999.679 kb on + strand at 999.679 kb on + strand at 999.679 kb on + strand at 999.679 kb on + strand at 999.679 kb on + strand at 999.680 kb on - strand at 999.680 kb on - strand at 999.734 kb on + strand at 999.734 kb on + strand at 999.735 kb on - strand at 999.735 kb on - strand at 999.735 kb on - strand at 999.735 kb on - strand at 999.735 kb on - strand at 1000.123 kb on + strand, within Psest_0919 at 1000.124 kb on - strand, within Psest_0919 at 1000.124 kb on - strand, within Psest_0919 at 1000.260 kb on + strand, within Psest_0919 at 1000.570 kb on + strand at 1000.571 kb on - strand at 1000.607 kb on + strand at 1000.608 kb on - strand at 1000.608 kb on - strand at 1000.608 kb on - strand at 1000.643 kb on + strand at 1000.644 kb on - strand at 1000.644 kb on - strand at 1000.647 kb on + strand at 1000.648 kb on - strand at 1000.648 kb on - strand at 1000.648 kb on - strand at 1000.648 kb on - strand at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.967 kb on + strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.968 kb on - strand, within Psest_0920 at 1000.997 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.059 kb on + strand, within Psest_0920 at 1001.060 kb on - strand, within Psest_0920 at 1001.060 kb on - strand, within Psest_0920 at 1001.083 kb on + strand, within Psest_0920 at 1001.084 kb on - strand, within Psest_0920 at 1001.084 kb on - strand, within Psest_0920 at 1001.204 kb on - strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.286 kb on + strand at 1001.287 kb on - strand at 1001.287 kb on - strand at 1001.289 kb on + strand at 1001.290 kb on - strand at 1001.290 kb on - strand at 1001.290 kb on - strand at 1001.313 kb on + strand at 1001.313 kb on + strand at 1001.313 kb on + strand at 1001.314 kb on - strand at 1001.314 kb on - strand at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.495 kb on + strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.496 kb on - strand, within Psest_0921 at 1001.499 kb on + strand, within Psest_0921 at 1001.500 kb on - strand, within Psest_0921 at 1001.500 kb on - strand, within Psest_0921
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 998,672 + +1.7 998,672 + +2.5 998,672 + +4.1 998,672 + +2.3 998,672 + -1.1 998,672 + +0.5 998,672 + +0.2 998,672 + +0.6 998,672 + -0.1 998,672 + -2.1 998,672 + +2.3 998,673 - +1.9 998,756 + Psest_0918 0.12 -0.9 998,756 + Psest_0918 0.12 -1.6 998,756 + Psest_0918 0.12 +0.5 998,756 + Psest_0918 0.12 +1.2 998,767 + Psest_0918 0.13 +2.7 998,861 + Psest_0918 0.23 +2.8 998,861 + Psest_0918 0.23 -0.3 998,862 - Psest_0918 0.23 -1.3 998,862 - Psest_0918 0.23 -0.2 998,862 - Psest_0918 0.23 +1.7 998,867 + Psest_0918 0.24 +1.3 998,954 + Psest_0918 0.33 -0.1 998,954 + Psest_0918 0.33 -2.8 998,954 + Psest_0918 0.33 -0.3 998,954 + Psest_0918 0.33 -0.8 998,954 + Psest_0918 0.33 -2.0 998,954 + Psest_0918 0.33 +1.5 998,954 + Psest_0918 0.33 +1.1 998,954 + Psest_0918 0.33 -1.4 998,954 + Psest_0918 0.33 +1.1 998,954 + Psest_0918 0.33 +3.3 998,954 + Psest_0918 0.33 -1.9 998,954 + Psest_0918 0.33 -0.7 998,954 + Psest_0918 0.33 -1.4 998,955 - Psest_0918 0.34 -2.4 998,955 - Psest_0918 0.34 -0.1 998,955 - Psest_0918 0.34 +1.8 998,955 - Psest_0918 0.34 -0.1 998,955 - Psest_0918 0.34 -1.1 998,955 - Psest_0918 0.34 +2.2 998,955 - Psest_0918 0.34 +1.0 998,955 - Psest_0918 0.34 +1.2 998,955 - Psest_0918 0.34 -1.3 998,955 - Psest_0918 0.34 -0.9 998,955 - Psest_0918 0.34 +0.9 998,955 - Psest_0918 0.34 -0.3 998,955 - Psest_0918 0.34 +1.1 999,047 - Psest_0918 0.44 -1.0 999,127 + Psest_0918 0.52 +0.9 999,127 + Psest_0918 0.52 -2.8 999,127 + Psest_0918 0.52 +0.7 999,127 + Psest_0918 0.52 +1.2 999,127 + Psest_0918 0.52 +0.1 999,127 + Psest_0918 0.52 -1.9 999,127 + Psest_0918 0.52 +1.3 999,127 + Psest_0918 0.52 +1.5 999,127 + Psest_0918 0.52 -0.6 999,127 + Psest_0918 0.52 +0.1 999,127 + Psest_0918 0.52 +0.4 999,127 + Psest_0918 0.52 -1.2 999,128 - Psest_0918 0.53 +1.1 999,128 - Psest_0918 0.53 +0.6 999,128 - Psest_0918 0.53 +0.3 999,156 + Psest_0918 0.56 -1.6 999,156 + Psest_0918 0.56 -0.6 999,156 + Psest_0918 0.56 +1.3 999,157 - Psest_0918 0.56 +1.0 999,160 + Psest_0918 0.56 -0.3 999,160 + Psest_0918 0.56 +0.4 999,160 + Psest_0918 0.56 +2.2 999,160 + Psest_0918 0.56 -0.0 999,161 - Psest_0918 0.56 -2.0 999,196 - Psest_0918 0.60 -1.4 999,237 + Psest_0918 0.65 +0.7 999,316 + Psest_0918 0.73 +0.5 999,317 - Psest_0918 0.73 +1.3 999,362 + Psest_0918 0.78 +0.5 999,373 + Psest_0918 0.80 +0.4 999,374 - Psest_0918 0.80 +1.4 999,374 - Psest_0918 0.80 -0.2 999,497 + +1.4 999,498 - -1.8 999,498 - +0.5 999,498 - +3.9 999,561 - -3.4 999,652 + -1.8 999,652 + +1.3 999,652 + +2.0 999,652 + -0.8 999,653 - +0.1 999,653 - +0.9 999,653 - +0.7 999,653 - -0.1 999,654 + -0.7 999,654 + -0.1 999,654 + -1.2 999,654 + -0.9 999,679 + +0.1 999,679 + -0.5 999,679 + +0.6 999,679 + +0.7 999,679 + -0.5 999,680 - +0.9 999,680 - -2.5 999,734 + -1.6 999,734 + -0.3 999,735 - -0.4 999,735 - +0.8 999,735 - -1.7 999,735 - +0.7 999,735 - -0.8 1,000,123 + Psest_0919 0.47 -2.8 1,000,124 - Psest_0919 0.47 -0.6 1,000,124 - Psest_0919 0.47 -0.8 1,000,260 + Psest_0919 0.62 -0.2 1,000,570 + -1.1 1,000,571 - -1.5 1,000,607 + -1.3 1,000,608 - +1.4 1,000,608 - -0.3 1,000,608 - +0.3 1,000,643 + +2.8 1,000,644 - -0.9 1,000,644 - +0.2 1,000,647 + -1.9 1,000,648 - -1.0 1,000,648 - -0.3 1,000,648 - -0.3 1,000,648 - -3.0 1,000,967 + Psest_0920 0.42 +1.9 1,000,967 + Psest_0920 0.42 +0.7 1,000,967 + Psest_0920 0.42 +3.8 1,000,967 + Psest_0920 0.42 -2.9 1,000,967 + Psest_0920 0.42 +0.2 1,000,967 + Psest_0920 0.42 +1.3 1,000,967 + Psest_0920 0.42 +1.0 1,000,967 + Psest_0920 0.42 +0.8 1,000,968 - Psest_0920 0.42 -1.5 1,000,968 - Psest_0920 0.42 -0.2 1,000,968 - Psest_0920 0.42 +2.4 1,000,968 - Psest_0920 0.42 -0.4 1,000,968 - Psest_0920 0.42 +3.1 1,000,968 - Psest_0920 0.42 +0.7 1,000,968 - Psest_0920 0.42 +0.8 1,000,997 + Psest_0920 0.48 -2.1 1,001,059 + Psest_0920 0.60 -1.3 1,001,059 + Psest_0920 0.60 -0.4 1,001,059 + Psest_0920 0.60 -0.6 1,001,059 + Psest_0920 0.60 +1.8 1,001,059 + Psest_0920 0.60 -0.2 1,001,060 - Psest_0920 0.61 +2.8 1,001,060 - Psest_0920 0.61 +0.4 1,001,083 + Psest_0920 0.65 -0.6 1,001,084 - Psest_0920 0.66 -2.1 1,001,084 - Psest_0920 0.66 +1.4 1,001,204 - +1.4 1,001,286 + -1.5 1,001,286 + +1.6 1,001,286 + -1.4 1,001,286 + -0.6 1,001,286 + -1.2 1,001,286 + -0.3 1,001,287 - -0.8 1,001,287 - +1.2 1,001,289 + -0.7 1,001,290 - -1.3 1,001,290 - +1.4 1,001,290 - -0.9 1,001,313 + +2.2 1,001,313 + -0.9 1,001,313 + +2.5 1,001,314 - -2.0 1,001,314 - -0.1 1,001,495 + Psest_0921 0.20 -0.3 1,001,495 + Psest_0921 0.20 +0.2 1,001,495 + Psest_0921 0.20 -3.5 1,001,495 + Psest_0921 0.20 +0.5 1,001,495 + Psest_0921 0.20 -1.8 1,001,496 - Psest_0921 0.20 +2.0 1,001,496 - Psest_0921 0.20 +2.2 1,001,496 - Psest_0921 0.20 -2.4 1,001,496 - Psest_0921 0.20 +0.4 1,001,496 - Psest_0921 0.20 -2.0 1,001,496 - Psest_0921 0.20 -1.9 1,001,496 - Psest_0921 0.20 +0.8 1,001,499 + Psest_0921 0.21 -0.4 1,001,500 - Psest_0921 0.21 -0.3 1,001,500 - Psest_0921 0.21 +2.0
Or see this region's nucleotide sequence