Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0856 and Psest_0857 are separated by 275 nucleotides Psest_0857 and Psest_0858 are separated by 251 nucleotides Psest_0858 and Psest_0859 are separated by 122 nucleotides
Psest_0856: Psest_0856 - Predicted oxidoreductase, at 927,168 to 928,031
_0856
Psest_0857: Psest_0857 - Membrane transporters of cations and cationic drugs, at 928,307 to 928,627
_0857
Psest_0858: Psest_0858 - Maltose operon periplasmic protein precursor (MalM)., at 928,879 to 929,802
_0858
Psest_0859: Psest_0859 - Maltoporin (phage lambda and maltose receptor), at 929,925 to 931,217
_0859
Position (kb)
928
929
930 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 927.993 kb on + strand at 927.993 kb on + strand at 927.993 kb on + strand at 927.993 kb on + strand at 928.101 kb on + strand at 928.102 kb on - strand at 928.102 kb on - strand at 928.102 kb on - strand at 928.104 kb on + strand at 928.105 kb on - strand at 928.105 kb on - strand at 928.105 kb on - strand at 928.168 kb on + strand at 928.227 kb on - strand at 928.488 kb on - strand, within Psest_0857 at 928.488 kb on - strand, within Psest_0857 at 928.668 kb on + strand at 928.668 kb on + strand at 928.669 kb on - strand at 928.669 kb on - strand at 928.718 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.747 kb on + strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.748 kb on - strand at 928.750 kb on - strand at 928.750 kb on - strand at 928.804 kb on + strand at 928.826 kb on + strand at 929.012 kb on + strand, within Psest_0858 at 929.012 kb on + strand, within Psest_0858 at 929.012 kb on + strand, within Psest_0858 at 929.013 kb on - strand, within Psest_0858 at 929.096 kb on - strand, within Psest_0858 at 929.099 kb on + strand, within Psest_0858 at 929.099 kb on + strand, within Psest_0858 at 929.099 kb on + strand, within Psest_0858 at 929.100 kb on - strand, within Psest_0858 at 929.158 kb on + strand, within Psest_0858 at 929.222 kb on + strand, within Psest_0858 at 929.279 kb on + strand, within Psest_0858 at 929.280 kb on - strand, within Psest_0858 at 929.381 kb on + strand, within Psest_0858 at 929.381 kb on + strand, within Psest_0858 at 929.381 kb on + strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.382 kb on - strand, within Psest_0858 at 929.477 kb on + strand, within Psest_0858 at 929.478 kb on - strand, within Psest_0858 at 929.478 kb on - strand, within Psest_0858 at 929.556 kb on - strand, within Psest_0858 at 929.912 kb on + strand at 929.912 kb on + strand at 929.912 kb on + strand at 929.912 kb on + strand at 929.912 kb on + strand at 929.912 kb on + strand at 929.913 kb on - strand at 929.913 kb on - strand at 929.927 kb on - strand at 930.004 kb on + strand at 930.028 kb on + strand at 930.028 kb on + strand at 930.028 kb on + strand at 930.028 kb on + strand at 930.028 kb on + strand at 930.028 kb on + strand at 930.028 kb on + strand at 930.029 kb on - strand at 930.029 kb on - strand at 930.029 kb on - strand at 930.029 kb on - strand at 930.196 kb on + strand, within Psest_0859 at 930.196 kb on + strand, within Psest_0859 at 930.196 kb on + strand, within Psest_0859 at 930.196 kb on + strand, within Psest_0859 at 930.197 kb on - strand, within Psest_0859 at 930.262 kb on + strand, within Psest_0859 at 930.262 kb on + strand, within Psest_0859 at 930.263 kb on - strand, within Psest_0859 at 930.263 kb on - strand, within Psest_0859 at 930.263 kb on - strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.274 kb on + strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.275 kb on - strand, within Psest_0859 at 930.415 kb on + strand, within Psest_0859 at 930.416 kb on - strand, within Psest_0859 at 930.478 kb on + strand, within Psest_0859 at 930.524 kb on - strand, within Psest_0859 at 930.730 kb on + strand, within Psest_0859 at 930.731 kb on - strand, within Psest_0859 at 930.731 kb on - strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.733 kb on + strand, within Psest_0859 at 930.734 kb on - strand, within Psest_0859 at 930.734 kb on - strand, within Psest_0859 at 930.734 kb on - strand, within Psest_0859 at 930.766 kb on + strand, within Psest_0859 at 930.766 kb on + strand, within Psest_0859 at 930.766 kb on + strand, within Psest_0859 at 930.766 kb on + strand, within Psest_0859 at 930.766 kb on + strand, within Psest_0859 at 930.766 kb on + strand, within Psest_0859 at 930.766 kb on + strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859 at 930.767 kb on - strand, within Psest_0859
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 927,993 + +3.0 927,993 + +1.2 927,993 + +1.2 927,993 + -2.4 928,101 + +2.4 928,102 - -0.3 928,102 - -2.6 928,102 - -1.9 928,104 + +0.5 928,105 - -2.3 928,105 - -0.8 928,105 - -1.0 928,168 + -0.7 928,227 - -0.8 928,488 - Psest_0857 0.56 -1.5 928,488 - Psest_0857 0.56 -1.7 928,668 + -1.5 928,668 + +2.0 928,669 - +1.4 928,669 - +0.9 928,718 + -0.3 928,747 + -0.5 928,747 + -0.8 928,747 + -2.7 928,747 + -1.4 928,747 + +2.0 928,747 + +1.3 928,748 - -2.8 928,748 - +0.4 928,748 - -1.4 928,748 - +0.8 928,748 - -0.6 928,748 - -0.6 928,748 - -2.4 928,748 - -1.0 928,748 - -1.5 928,748 - -0.3 928,748 - -0.0 928,748 - +0.5 928,750 - -0.3 928,750 - -0.6 928,804 + -1.5 928,826 + +0.5 929,012 + Psest_0858 0.14 +1.3 929,012 + Psest_0858 0.14 +0.1 929,012 + Psest_0858 0.14 -1.1 929,013 - Psest_0858 0.15 +1.1 929,096 - Psest_0858 0.23 +1.3 929,099 + Psest_0858 0.24 -0.9 929,099 + Psest_0858 0.24 -1.1 929,099 + Psest_0858 0.24 -2.1 929,100 - Psest_0858 0.24 -2.0 929,158 + Psest_0858 0.30 -1.6 929,222 + Psest_0858 0.37 -1.8 929,279 + Psest_0858 0.43 -1.1 929,280 - Psest_0858 0.43 -1.7 929,381 + Psest_0858 0.54 -1.4 929,381 + Psest_0858 0.54 -1.3 929,381 + Psest_0858 0.54 -0.3 929,382 - Psest_0858 0.54 -0.3 929,382 - Psest_0858 0.54 -1.6 929,382 - Psest_0858 0.54 +0.7 929,382 - +1.6 929,382 - Psest_0858 0.54 -1.7 929,382 - Psest_0858 0.54 -1.9 929,382 - Psest_0858 0.54 +0.9 929,382 - Psest_0858 0.54 -0.1 929,382 - Psest_0858 0.54 -0.6 929,382 - Psest_0858 0.54 -2.1 929,477 + Psest_0858 0.65 -1.0 929,478 - Psest_0858 0.65 +0.7 929,478 - Psest_0858 0.65 -1.1 929,556 - Psest_0858 0.73 -0.4 929,912 + -0.0 929,912 + -1.8 929,912 + +3.5 929,912 + +4.4 929,912 + +0.9 929,912 + -0.0 929,913 - -1.0 929,913 - +2.7 929,927 - -0.4 930,004 + +0.3 930,028 + -1.1 930,028 + -2.8 930,028 + +1.7 930,028 + +1.6 930,028 + -0.4 930,028 + +1.4 930,028 + -1.5 930,029 - -1.1 930,029 - -2.2 930,029 - -0.4 930,029 - +0.0 930,196 + Psest_0859 0.21 -1.4 930,196 + Psest_0859 0.21 +0.5 930,196 + Psest_0859 0.21 +1.0 930,196 + Psest_0859 0.21 -1.0 930,197 - Psest_0859 0.21 -0.0 930,262 + Psest_0859 0.26 -1.1 930,262 + Psest_0859 0.26 +0.5 930,263 - Psest_0859 0.26 +0.7 930,263 - Psest_0859 0.26 +1.6 930,263 - Psest_0859 0.26 -0.8 930,274 + Psest_0859 0.27 -0.9 930,274 + Psest_0859 0.27 -1.5 930,274 + Psest_0859 0.27 -0.9 930,274 + Psest_0859 0.27 -2.9 930,274 + Psest_0859 0.27 -0.8 930,274 + Psest_0859 0.27 -0.4 930,274 + Psest_0859 0.27 -1.4 930,274 + Psest_0859 0.27 -0.1 930,274 + Psest_0859 0.27 -1.8 930,274 + Psest_0859 0.27 +0.6 930,274 + Psest_0859 0.27 -2.4 930,274 + Psest_0859 0.27 +3.0 930,274 + Psest_0859 0.27 -1.0 930,275 - Psest_0859 0.27 -0.4 930,275 - Psest_0859 0.27 -0.9 930,275 - Psest_0859 0.27 -1.0 930,275 - Psest_0859 0.27 -1.8 930,275 - Psest_0859 0.27 -0.7 930,275 - Psest_0859 0.27 -0.6 930,275 - Psest_0859 0.27 +0.4 930,275 - Psest_0859 0.27 -0.0 930,275 - Psest_0859 0.27 -2.4 930,275 - Psest_0859 0.27 -1.4 930,275 - Psest_0859 0.27 -1.4 930,415 + Psest_0859 0.38 -2.7 930,416 - Psest_0859 0.38 -2.0 930,478 + Psest_0859 0.43 +1.8 930,524 - Psest_0859 0.46 -0.5 930,730 + Psest_0859 0.62 -0.3 930,731 - Psest_0859 0.62 -0.9 930,731 - Psest_0859 0.62 +0.6 930,733 + Psest_0859 0.62 -4.0 930,733 + Psest_0859 0.62 -1.0 930,733 + Psest_0859 0.62 -1.4 930,733 + Psest_0859 0.62 -0.6 930,733 + Psest_0859 0.62 -0.0 930,733 + Psest_0859 0.62 -1.1 930,733 + Psest_0859 0.62 -2.2 930,733 + Psest_0859 0.62 -0.2 930,733 + Psest_0859 0.62 -0.3 930,733 + Psest_0859 0.62 -0.0 930,733 + Psest_0859 0.62 -0.9 930,734 - Psest_0859 0.63 -0.3 930,734 - Psest_0859 0.63 -1.4 930,734 - Psest_0859 0.63 -3.0 930,766 + Psest_0859 0.65 -3.4 930,766 + Psest_0859 0.65 -1.4 930,766 + Psest_0859 0.65 +0.4 930,766 + Psest_0859 0.65 -3.7 930,766 + Psest_0859 0.65 +2.3 930,766 + Psest_0859 0.65 -1.3 930,766 + Psest_0859 0.65 -3.0 930,767 - Psest_0859 0.65 -1.0 930,767 - Psest_0859 0.65 +1.0 930,767 - Psest_0859 0.65 +0.2 930,767 - Psest_0859 0.65 +1.5 930,767 - Psest_0859 0.65 +1.0 930,767 - Psest_0859 0.65 +1.1 930,767 - Psest_0859 0.65 +1.0 930,767 - Psest_0859 0.65 +1.2
Or see this region's nucleotide sequence