Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0754 and Psest_0755 are separated by 92 nucleotides Psest_0755 and Psest_0756 are separated by 94 nucleotides Psest_0756 and Psest_0757 are separated by 42 nucleotides Psest_0757 and Psest_0758 are separated by 114 nucleotides
Psest_0754: Psest_0754 - Predicted transcriptional regulators, at 825,903 to 826,208
_0754
Psest_0755: Psest_0755 - Bacterial regulatory helix-turn-helix proteins, AraC family., at 826,301 to 827,098
_0755
Psest_0756: Psest_0756 - alkylhydroperoxidase AhpD family core domain, at 827,193 to 827,651
_0756
Psest_0757: Psest_0757 - hypothetical protein, at 827,694 to 828,167
_0757
Psest_0758: Psest_0758 - Lactoylglutathione lyase and related lyases, at 828,282 to 828,671
_0758
Position (kb)
827
828 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 826.493 kb on + strand, within Psest_0755 at 826.493 kb on + strand, within Psest_0755 at 826.493 kb on + strand, within Psest_0755 at 826.493 kb on + strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.494 kb on - strand, within Psest_0755 at 826.598 kb on + strand, within Psest_0755 at 826.598 kb on + strand, within Psest_0755 at 826.598 kb on + strand, within Psest_0755 at 826.599 kb on - strand, within Psest_0755 at 826.599 kb on - strand, within Psest_0755 at 826.744 kb on + strand, within Psest_0755 at 826.745 kb on - strand, within Psest_0755 at 826.745 kb on - strand, within Psest_0755 at 826.745 kb on - strand, within Psest_0755 at 826.977 kb on + strand, within Psest_0755 at 826.977 kb on + strand, within Psest_0755 at 826.977 kb on + strand, within Psest_0755 at 826.977 kb on + strand, within Psest_0755 at 826.978 kb on - strand, within Psest_0755 at 827.022 kb on + strand at 827.022 kb on + strand at 827.022 kb on + strand at 827.022 kb on + strand at 827.023 kb on - strand at 827.130 kb on - strand at 827.134 kb on + strand at 827.134 kb on + strand at 827.134 kb on + strand at 827.135 kb on - strand at 827.135 kb on - strand at 827.135 kb on - strand at 827.135 kb on - strand at 827.135 kb on - strand at 827.205 kb on + strand at 827.205 kb on + strand at 827.206 kb on - strand at 827.257 kb on - strand, within Psest_0756 at 827.409 kb on + strand, within Psest_0756 at 827.409 kb on + strand, within Psest_0756 at 827.409 kb on + strand, within Psest_0756 at 827.409 kb on + strand, within Psest_0756 at 827.409 kb on + strand, within Psest_0756 at 827.409 kb on + strand, within Psest_0756 at 827.409 kb on + strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.410 kb on - strand, within Psest_0756 at 827.637 kb on + strand at 827.637 kb on + strand at 827.638 kb on - strand at 827.840 kb on - strand, within Psest_0757 at 827.852 kb on - strand, within Psest_0757 at 827.912 kb on + strand, within Psest_0757 at 828.016 kb on - strand, within Psest_0757 at 828.094 kb on + strand, within Psest_0757 at 828.095 kb on - strand, within Psest_0757 at 828.095 kb on - strand, within Psest_0757 at 828.095 kb on - strand, within Psest_0757 at 828.095 kb on - strand, within Psest_0757 at 828.355 kb on + strand, within Psest_0758 at 828.356 kb on - strand, within Psest_0758 at 828.356 kb on - strand, within Psest_0758 at 828.356 kb on - strand, within Psest_0758 at 828.547 kb on + strand, within Psest_0758 at 828.547 kb on + strand, within Psest_0758 at 828.548 kb on - strand, within Psest_0758 at 828.548 kb on - strand, within Psest_0758 at 828.548 kb on - strand, within Psest_0758 at 828.548 kb on - strand, within Psest_0758 at 828.607 kb on + strand, within Psest_0758 at 828.607 kb on + strand, within Psest_0758 at 828.607 kb on + strand, within Psest_0758 at 828.607 kb on + strand, within Psest_0758 at 828.608 kb on - strand, within Psest_0758 at 828.608 kb on - strand, within Psest_0758 at 828.608 kb on - strand, within Psest_0758 at 828.608 kb on - strand, within Psest_0758 at 828.608 kb on - strand, within Psest_0758 at 828.608 kb on - strand, within Psest_0758 at 828.629 kb on - strand, within Psest_0758 at 828.629 kb on - strand, within Psest_0758 at 828.629 kb on - strand, within Psest_0758
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 826,493 + Psest_0755 0.24 -0.4 826,493 + Psest_0755 0.24 +0.1 826,493 + Psest_0755 0.24 -0.3 826,493 + Psest_0755 0.24 +2.9 826,494 - Psest_0755 0.24 -0.2 826,494 - Psest_0755 0.24 +1.8 826,494 - Psest_0755 0.24 -1.4 826,494 - Psest_0755 0.24 +0.0 826,494 - Psest_0755 0.24 -0.2 826,494 - Psest_0755 0.24 +1.4 826,494 - Psest_0755 0.24 -1.5 826,494 - Psest_0755 0.24 -0.4 826,494 - Psest_0755 0.24 -0.2 826,598 + Psest_0755 0.37 -1.1 826,598 + Psest_0755 0.37 +1.3 826,598 + Psest_0755 0.37 +0.2 826,599 - Psest_0755 0.37 +0.9 826,599 - Psest_0755 0.37 +0.1 826,744 + Psest_0755 0.56 -0.1 826,745 - Psest_0755 0.56 -1.1 826,745 - Psest_0755 0.56 -0.0 826,745 - Psest_0755 0.56 -0.2 826,977 + Psest_0755 0.85 +0.0 826,977 + Psest_0755 0.85 -1.3 826,977 + Psest_0755 0.85 +0.6 826,977 + Psest_0755 0.85 -0.4 826,978 - Psest_0755 0.85 +1.2 827,022 + -1.9 827,022 + +1.6 827,022 + -2.0 827,022 + -1.4 827,023 - +1.2 827,130 - -0.3 827,134 + +0.1 827,134 + -0.3 827,134 + +0.1 827,135 - +1.0 827,135 - -0.3 827,135 - -3.3 827,135 - +2.2 827,135 - +1.4 827,205 + -0.1 827,205 + -0.9 827,206 - +0.5 827,257 - Psest_0756 0.14 +0.2 827,409 + Psest_0756 0.47 -1.4 827,409 + Psest_0756 0.47 -0.8 827,409 + Psest_0756 0.47 +0.4 827,409 + Psest_0756 0.47 -1.2 827,409 + Psest_0756 0.47 -0.0 827,409 + Psest_0756 0.47 -0.0 827,409 + Psest_0756 0.47 +2.1 827,410 - Psest_0756 0.47 -1.0 827,410 - Psest_0756 0.47 -0.3 827,410 - Psest_0756 0.47 -1.0 827,410 - Psest_0756 0.47 -2.2 827,410 - Psest_0756 0.47 +1.8 827,410 - Psest_0756 0.47 -0.8 827,410 - Psest_0756 0.47 +1.0 827,410 - Psest_0756 0.47 -0.4 827,637 + -0.9 827,637 + +1.3 827,638 - +0.5 827,840 - Psest_0757 0.31 -1.8 827,852 - Psest_0757 0.33 +0.4 827,912 + Psest_0757 0.46 -1.5 828,016 - Psest_0757 0.68 -2.3 828,094 + Psest_0757 0.84 -1.5 828,095 - Psest_0757 0.85 -2.2 828,095 - Psest_0757 0.85 -1.0 828,095 - Psest_0757 0.85 -1.4 828,095 - Psest_0757 0.85 +0.7 828,355 + Psest_0758 0.19 -2.4 828,356 - Psest_0758 0.19 +3.6 828,356 - Psest_0758 0.19 +0.5 828,356 - Psest_0758 0.19 +0.2 828,547 + Psest_0758 0.68 -1.4 828,547 + Psest_0758 0.68 -0.1 828,548 - Psest_0758 0.68 -1.0 828,548 - Psest_0758 0.68 -2.4 828,548 - Psest_0758 0.68 -1.2 828,548 - Psest_0758 0.68 +1.6 828,607 + Psest_0758 0.83 -2.2 828,607 + Psest_0758 0.83 -1.9 828,607 + Psest_0758 0.83 +2.7 828,607 + Psest_0758 0.83 -1.4 828,608 - Psest_0758 0.84 -0.8 828,608 - Psest_0758 0.84 +1.4 828,608 - Psest_0758 0.84 +0.9 828,608 - Psest_0758 0.84 +2.3 828,608 - Psest_0758 0.84 +3.1 828,608 - Psest_0758 0.84 -0.3 828,629 - Psest_0758 0.89 -1.3 828,629 - Psest_0758 0.89 -0.4 828,629 - Psest_0758 0.89 +1.4
Or see this region's nucleotide sequence