Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0733 and Psest_0734 are separated by 4 nucleotides Psest_0734 and Psest_0735 are separated by 248 nucleotides Psest_0735 and Psest_0736 are separated by 49 nucleotides
Psest_0733: Psest_0733 - Cation/multidrug efflux pump, at 803,249 to 806,293
_0733
Psest_0734: Psest_0734 - RND family efflux transporter, MFP subunit, at 806,298 to 807,377
_0734
Psest_0735: Psest_0735 - bacterioferritin, at 807,626 to 808,093
_0735
Psest_0736: Psest_0736 - Methyl-accepting chemotaxis protein, at 808,143 to 810,065
_0736
Position (kb) 806
807
808 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 805.332 kb on + strand, within Psest_0733 at 805.332 kb on + strand, within Psest_0733 at 805.333 kb on - strand, within Psest_0733 at 805.399 kb on + strand, within Psest_0733 at 805.400 kb on - strand, within Psest_0733 at 805.500 kb on + strand, within Psest_0733 at 805.500 kb on + strand, within Psest_0733 at 805.500 kb on + strand, within Psest_0733 at 805.866 kb on + strand, within Psest_0733 at 805.878 kb on + strand, within Psest_0733 at 805.879 kb on - strand, within Psest_0733 at 806.240 kb on + strand at 806.278 kb on - strand at 806.278 kb on - strand at 806.430 kb on + strand, within Psest_0734 at 806.633 kb on - strand, within Psest_0734 at 806.674 kb on + strand, within Psest_0734 at 806.674 kb on + strand, within Psest_0734 at 806.674 kb on + strand, within Psest_0734 at 806.674 kb on + strand, within Psest_0734 at 807.119 kb on + strand, within Psest_0734 at 807.120 kb on - strand, within Psest_0734 at 807.436 kb on + strand at 807.436 kb on + strand at 807.437 kb on - strand at 807.437 kb on - strand at 807.437 kb on - strand at 807.595 kb on + strand at 807.595 kb on + strand at 807.621 kb on - strand at 807.654 kb on - strand at 807.699 kb on - strand, within Psest_0735 at 807.699 kb on - strand, within Psest_0735 at 807.719 kb on + strand, within Psest_0735 at 807.720 kb on - strand, within Psest_0735 at 807.720 kb on - strand, within Psest_0735 at 807.720 kb on - strand, within Psest_0735 at 807.746 kb on + strand, within Psest_0735 at 807.746 kb on + strand, within Psest_0735 at 807.746 kb on + strand, within Psest_0735 at 807.746 kb on + strand, within Psest_0735 at 807.747 kb on - strand, within Psest_0735 at 807.798 kb on + strand, within Psest_0735 at 807.798 kb on + strand, within Psest_0735 at 807.798 kb on + strand, within Psest_0735 at 807.905 kb on + strand, within Psest_0735 at 807.905 kb on + strand, within Psest_0735 at 807.905 kb on + strand, within Psest_0735 at 807.906 kb on - strand, within Psest_0735 at 807.906 kb on - strand, within Psest_0735 at 807.906 kb on - strand, within Psest_0735 at 807.906 kb on - strand, within Psest_0735 at 808.020 kb on - strand, within Psest_0735 at 808.070 kb on + strand at 808.071 kb on - strand at 808.113 kb on + strand at 808.113 kb on + strand at 808.113 kb on + strand at 808.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 805,332 + Psest_0733 0.68 +0.3 805,332 + Psest_0733 0.68 +3.1 805,333 - Psest_0733 0.68 +0.2 805,399 + Psest_0733 0.71 -1.8 805,400 - Psest_0733 0.71 +1.7 805,500 + Psest_0733 0.74 -0.8 805,500 + Psest_0733 0.74 -0.7 805,500 + Psest_0733 0.74 +0.8 805,866 + Psest_0733 0.86 -0.4 805,878 + Psest_0733 0.86 -0.1 805,879 - Psest_0733 0.86 -0.6 806,240 + +0.7 806,278 - -2.1 806,278 - -1.0 806,430 + Psest_0734 0.12 -1.2 806,633 - Psest_0734 0.31 +0.3 806,674 + Psest_0734 0.35 -2.3 806,674 + Psest_0734 0.35 -3.4 806,674 + Psest_0734 0.35 -1.0 806,674 + Psest_0734 0.35 +1.3 807,119 + Psest_0734 0.76 +0.4 807,120 - Psest_0734 0.76 -1.5 807,436 + +0.8 807,436 + +1.8 807,437 - -0.2 807,437 - -0.7 807,437 - -1.1 807,595 + +1.8 807,595 + -2.6 807,621 - -0.9 807,654 - +3.5 807,699 - Psest_0735 0.16 -0.9 807,699 - Psest_0735 0.16 +1.1 807,719 + Psest_0735 0.20 +1.2 807,720 - Psest_0735 0.20 -0.4 807,720 - Psest_0735 0.20 -1.1 807,720 - Psest_0735 0.20 +1.3 807,746 + Psest_0735 0.26 -0.3 807,746 + Psest_0735 0.26 +1.9 807,746 + Psest_0735 0.26 +0.1 807,746 + Psest_0735 0.26 -1.6 807,747 - Psest_0735 0.26 +1.2 807,798 + Psest_0735 0.37 +0.3 807,798 + Psest_0735 0.37 -1.1 807,798 + Psest_0735 0.37 -2.7 807,905 + Psest_0735 0.60 -0.2 807,905 + Psest_0735 0.60 -3.2 807,905 + Psest_0735 0.60 -2.4 807,906 - Psest_0735 0.60 +0.6 807,906 - Psest_0735 0.60 -2.7 807,906 - Psest_0735 0.60 +0.1 807,906 - Psest_0735 0.60 +1.9 808,020 - Psest_0735 0.84 +0.9 808,070 + +0.3 808,071 - -0.7 808,113 + -2.6 808,113 + +0.7 808,113 + -0.0 808,113 + +1.4
Or see this region's nucleotide sequence