Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0063 and Psest_0064 are separated by 171 nucleotides Psest_0064 and Psest_0065 are separated by 271 nucleotides
Psest_0063: Psest_0063 - tryptophan synthase, beta subunit, at 62,768 to 63,979
_0063
Psest_0064: Psest_0064 - Transcriptional regulator, at 64,151 to 65,053
_0064
Psest_0065: Psest_0065 - Subtilisin-like serine proteases, at 65,325 to 66,716
_0065
Position (kb) 64
65
66 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 63.405 kb on + strand, within Psest_0063 at 63.405 kb on + strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.406 kb on - strand, within Psest_0063 at 63.564 kb on + strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.565 kb on - strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.741 kb on + strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.742 kb on - strand, within Psest_0063 at 63.787 kb on - strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.810 kb on + strand, within Psest_0063 at 63.811 kb on - strand, within Psest_0063 at 63.811 kb on - strand, within Psest_0063 at 63.826 kb on - strand, within Psest_0063 at 63.826 kb on - strand, within Psest_0063 at 64.086 kb on + strand at 64.150 kb on + strand at 64.150 kb on + strand at 64.209 kb on + strand at 64.210 kb on - strand at 64.210 kb on - strand at 64.210 kb on - strand at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.829 kb on + strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 64.830 kb on - strand, within Psest_0064 at 65.099 kb on + strand at 65.099 kb on + strand at 65.179 kb on + strand at 65.246 kb on + strand at 65.246 kb on + strand at 65.247 kb on - strand at 65.247 kb on - strand at 65.247 kb on - strand at 65.269 kb on + strand at 65.269 kb on + strand at 65.269 kb on + strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.270 kb on - strand at 65.683 kb on - strand, within Psest_0065 at 65.724 kb on - strand, within Psest_0065 at 65.769 kb on + strand, within Psest_0065 at 65.994 kb on + strand, within Psest_0065
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 63,405 + Psest_0063 0.53 -1.0 63,405 + Psest_0063 0.53 +1.3 63,406 - Psest_0063 0.53 -2.0 63,406 - Psest_0063 0.53 -0.8 63,406 - Psest_0063 0.53 +0.4 63,406 - Psest_0063 0.53 +4.6 63,406 - Psest_0063 0.53 -1.5 63,406 - Psest_0063 0.53 +2.6 63,406 - Psest_0063 0.53 +0.0 63,406 - Psest_0063 0.53 -0.8 63,564 + Psest_0063 0.66 -0.2 63,565 - Psest_0063 0.66 +2.6 63,565 - Psest_0063 0.66 -0.6 63,565 - Psest_0063 0.66 +4.5 63,565 - Psest_0063 0.66 -1.2 63,565 - Psest_0063 0.66 +1.8 63,741 + Psest_0063 0.80 -1.5 63,741 + Psest_0063 0.80 -2.3 63,741 + Psest_0063 0.80 -0.2 63,741 + Psest_0063 0.80 -2.3 63,741 + Psest_0063 0.80 -2.5 63,742 - Psest_0063 0.80 +1.3 63,742 - Psest_0063 0.80 +1.3 63,742 - Psest_0063 0.80 +0.0 63,742 - Psest_0063 0.80 +0.0 63,787 - Psest_0063 0.84 +0.3 63,810 + Psest_0063 0.86 +0.3 63,810 + Psest_0063 0.86 -0.5 63,810 + Psest_0063 0.86 -2.6 63,810 + Psest_0063 0.86 +1.0 63,810 + Psest_0063 0.86 +4.2 63,810 + Psest_0063 0.86 -1.1 63,810 + Psest_0063 0.86 -1.7 63,810 + Psest_0063 0.86 +1.4 63,811 - Psest_0063 0.86 -0.8 63,811 - Psest_0063 0.86 -2.1 63,826 - Psest_0063 0.87 -0.9 63,826 - Psest_0063 0.87 +3.9 64,086 + +0.0 64,150 + -1.6 64,150 + -1.4 64,209 + -0.4 64,210 - +2.0 64,210 - -3.4 64,210 - -0.2 64,829 + Psest_0064 0.75 -2.4 64,829 + Psest_0064 0.75 -0.5 64,829 + Psest_0064 0.75 -1.9 64,829 + Psest_0064 0.75 -1.5 64,830 - Psest_0064 0.75 -2.8 64,830 - Psest_0064 0.75 -1.4 64,830 - Psest_0064 0.75 -1.5 64,830 - Psest_0064 0.75 +0.0 64,830 - Psest_0064 0.75 -0.4 65,099 + -3.4 65,099 + -0.1 65,179 + -0.5 65,246 + -0.5 65,246 + +3.2 65,247 - -0.7 65,247 - -1.5 65,247 - -0.8 65,269 + -3.5 65,269 + +5.5 65,269 + +3.4 65,270 - +1.4 65,270 - +1.1 65,270 - -1.0 65,270 - -1.4 65,683 - Psest_0065 0.26 -2.7 65,724 - Psest_0065 0.29 -2.0 65,769 + Psest_0065 0.32 +0.6 65,994 + Psest_0065 0.48 -1.5
Or see this region's nucleotide sequence