Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0503 and Psest_0504 are separated by 225 nucleotides Psest_0504 and Psest_0505 overlap by 4 nucleotides Psest_0505 and Psest_0506 are separated by 10 nucleotides Psest_0506 and Psest_0507 are separated by 27 nucleotides
Psest_0503: Psest_0503 - Acyl dehydratase, at 558,058 to 558,513
_0503
Psest_0504: Psest_0504 - Putative effector of murein hydrolase LrgA, at 558,739 to 559,101
_0504
Psest_0505: Psest_0505 - Putative effector of murein hydrolase, at 559,098 to 559,817
_0505
Psest_0506: Psest_0506 - Uncharacterized protein, similar to the N-terminal domain of Lon protease, at 559,828 to 560,412
_0506
Psest_0507: Psest_0507 - Uncharacterized membrane-associated protein, at 560,440 to 561,759
_0507
Position (kb) 559
560 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 558.196 kb on + strand, within Psest_0503 at 558.268 kb on - strand, within Psest_0503 at 558.480 kb on - strand at 558.480 kb on - strand at 558.480 kb on - strand at 558.480 kb on - strand at 558.535 kb on + strand at 558.535 kb on + strand at 558.536 kb on - strand at 558.536 kb on - strand at 558.536 kb on - strand at 558.562 kb on - strand at 558.617 kb on - strand at 558.617 kb on - strand at 558.617 kb on - strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.711 kb on + strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.712 kb on - strand at 558.920 kb on - strand, within Psest_0504 at 558.964 kb on + strand, within Psest_0504 at 558.964 kb on + strand, within Psest_0504 at 558.965 kb on - strand, within Psest_0504 at 558.965 kb on - strand, within Psest_0504 at 558.966 kb on + strand, within Psest_0504 at 558.966 kb on + strand, within Psest_0504 at 558.966 kb on + strand, within Psest_0504 at 558.967 kb on - strand, within Psest_0504 at 559.116 kb on + strand at 559.117 kb on - strand at 559.166 kb on + strand at 559.167 kb on - strand at 559.167 kb on - strand at 559.191 kb on + strand, within Psest_0505 at 559.192 kb on - strand, within Psest_0505 at 559.192 kb on - strand, within Psest_0505 at 559.219 kb on + strand, within Psest_0505 at 559.287 kb on + strand, within Psest_0505 at 559.288 kb on - strand, within Psest_0505 at 559.288 kb on - strand, within Psest_0505 at 559.296 kb on + strand, within Psest_0505 at 559.859 kb on + strand at 559.860 kb on - strand at 559.860 kb on - strand at 559.860 kb on - strand at 559.860 kb on - strand at 559.906 kb on + strand, within Psest_0506 at 559.907 kb on - strand, within Psest_0506 at 559.955 kb on + strand, within Psest_0506 at 559.955 kb on + strand, within Psest_0506 at 559.956 kb on - strand, within Psest_0506 at 560.299 kb on + strand, within Psest_0506 at 560.453 kb on + strand at 560.453 kb on + strand at 560.454 kb on - strand at 560.454 kb on - strand at 560.654 kb on + strand, within Psest_0507 at 560.654 kb on + strand, within Psest_0507 at 560.655 kb on - strand, within Psest_0507 at 560.805 kb on - strand, within Psest_0507 at 560.805 kb on - strand, within Psest_0507
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 558,196 + Psest_0503 0.30 -0.1 558,268 - Psest_0503 0.46 +0.2 558,480 - -1.1 558,480 - -1.8 558,480 - -1.0 558,480 - +0.6 558,535 + -0.2 558,535 + -2.6 558,536 - -1.7 558,536 - +2.9 558,536 - -2.7 558,562 - +0.2 558,617 - -1.2 558,617 - -1.1 558,617 - +0.8 558,711 + +1.6 558,711 + -0.6 558,711 + +1.4 558,711 + -2.3 558,711 + -0.2 558,711 + +1.8 558,711 + -0.4 558,711 + -1.5 558,711 + -0.8 558,711 + +1.3 558,712 - -1.4 558,712 - +0.7 558,712 - -0.2 558,712 - +0.2 558,712 - +2.2 558,712 - -1.0 558,712 - -1.0 558,920 - Psest_0504 0.50 +0.9 558,964 + Psest_0504 0.62 -0.3 558,964 + Psest_0504 0.62 +2.6 558,965 - Psest_0504 0.62 +0.1 558,965 - Psest_0504 0.62 +1.1 558,966 + Psest_0504 0.63 -0.6 558,966 + Psest_0504 0.63 +0.6 558,966 + Psest_0504 0.63 +0.1 558,967 - Psest_0504 0.63 -0.5 559,116 + +1.3 559,117 - +0.0 559,166 + -1.4 559,167 - -0.9 559,167 - -0.9 559,191 + Psest_0505 0.13 -0.8 559,192 - Psest_0505 0.13 -0.2 559,192 - Psest_0505 0.13 -0.5 559,219 + Psest_0505 0.17 -1.3 559,287 + Psest_0505 0.26 -2.5 559,288 - Psest_0505 0.26 -1.9 559,288 - Psest_0505 0.26 -2.0 559,296 + Psest_0505 0.28 +0.5 559,859 + +2.3 559,860 - -1.3 559,860 - +1.1 559,860 - +0.6 559,860 - -0.4 559,906 + Psest_0506 0.13 +0.7 559,907 - Psest_0506 0.14 +0.6 559,955 + Psest_0506 0.22 +2.0 559,955 + Psest_0506 0.22 -0.4 559,956 - Psest_0506 0.22 -3.0 560,299 + Psest_0506 0.81 -0.6 560,453 + -0.7 560,453 + +1.0 560,454 - -0.0 560,454 - +1.9 560,654 + Psest_0507 0.16 +1.6 560,654 + Psest_0507 0.16 -1.8 560,655 - Psest_0507 0.16 +3.1 560,805 - Psest_0507 0.28 +1.0 560,805 - Psest_0507 0.28 +4.4
Or see this region's nucleotide sequence