Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0499 and Psest_0500 are separated by 28 nucleotides Psest_0500 and Psest_0501 are separated by 186 nucleotides Psest_0501 and Psest_0502 are separated by 19 nucleotides
Psest_0499: Psest_0499 - hypothetical protein, at 554,082 to 554,888
_0499
Psest_0500: Psest_0500 - Oligoribonuclease (3'->5' exoribonuclease), at 554,917 to 555,459
_0500
Psest_0501: Psest_0501 - Predicted nucleoside-diphosphate-sugar epimerases, at 555,646 to 556,287
_0501
Psest_0502: Psest_0502 - Uncharacterized protein conserved in bacteria, at 556,307 to 557,980
_0502
Position (kb) 555
556
557 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 554.668 kb on - strand, within Psest_0499 at 554.668 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 555.290 kb on - strand, within Psest_0500 at 555.290 kb on - strand, within Psest_0500 at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.560 kb on + strand at 555.560 kb on + strand at 555.560 kb on + strand at 555.560 kb on + strand at 555.561 kb on - strand at 555.561 kb on - strand at 555.561 kb on - strand at 555.758 kb on + strand, within Psest_0501 at 555.758 kb on + strand, within Psest_0501 at 555.759 kb on - strand, within Psest_0501 at 555.914 kb on + strand, within Psest_0501 at 555.914 kb on + strand, within Psest_0501 at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.393 kb on - strand at 556.393 kb on - strand at 556.500 kb on + strand, within Psest_0502 at 556.500 kb on + strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.608 kb on + strand, within Psest_0502 at 556.625 kb on + strand, within Psest_0502 at 556.626 kb on - strand, within Psest_0502 at 556.626 kb on - strand, within Psest_0502 at 556.626 kb on - strand, within Psest_0502 at 556.716 kb on - strand, within Psest_0502 at 556.752 kb on + strand, within Psest_0502 at 556.753 kb on - strand, within Psest_0502 at 556.905 kb on - strand, within Psest_0502 at 556.917 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.973 kb on - strand, within Psest_0502 at 556.973 kb on - strand, within Psest_0502 at 556.973 kb on - strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.975 kb on - strand, within Psest_0502 at 556.975 kb on - strand, within Psest_0502 at 556.975 kb on - strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.235 kb on + strand, within Psest_0502 at 557.262 kb on + strand, within Psest_0502 at 557.263 kb on - strand, within Psest_0502
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 554,668 - Psest_0499 0.73 -2.8 554,668 - Psest_0499 0.73 -0.7 554,680 - Psest_0499 0.74 -1.3 554,680 - Psest_0499 0.74 +1.6 554,680 - Psest_0499 0.74 +0.2 555,290 - Psest_0500 0.69 +0.2 555,290 - Psest_0500 0.69 +2.9 555,541 + -2.2 555,541 + +1.2 555,541 + -0.4 555,541 + +0.2 555,541 + -2.2 555,541 + -1.3 555,541 + -1.8 555,541 + -2.6 555,541 + -1.4 555,542 - -1.2 555,542 - +3.6 555,542 - +1.1 555,542 - +1.2 555,542 - +3.7 555,542 - -2.8 555,542 - +1.8 555,560 + -0.2 555,560 + +0.4 555,560 + -1.4 555,560 + -2.3 555,561 - -1.1 555,561 - -1.7 555,561 - +1.1 555,758 + Psest_0501 0.17 -0.8 555,758 + Psest_0501 0.17 -2.6 555,759 - Psest_0501 0.18 -0.1 555,914 + Psest_0501 0.42 -2.1 555,914 + Psest_0501 0.42 -0.6 556,283 + -2.6 556,283 + +0.2 556,283 + -0.5 556,283 + +1.6 556,283 + -0.2 556,283 + -1.8 556,283 + -2.1 556,283 + -2.4 556,283 + +0.5 556,284 - -0.7 556,284 - +0.6 556,284 - -3.0 556,284 - -0.0 556,284 - -0.9 556,284 - +1.0 556,284 - +0.1 556,284 - +1.5 556,284 - +0.5 556,284 - +0.7 556,284 - -0.0 556,284 - -1.8 556,284 - -0.9 556,284 - +0.4 556,284 - +3.2 556,284 - +0.1 556,284 - +2.3 556,284 - -1.8 556,284 - -1.0 556,284 - +0.8 556,284 - -1.2 556,284 - +0.1 556,284 - -0.6 556,284 - -1.4 556,284 - -2.7 556,284 - -1.2 556,284 - +0.6 556,393 - +0.3 556,393 - -0.6 556,500 + Psest_0502 0.12 -0.4 556,500 + Psest_0502 0.12 -2.7 556,501 - Psest_0502 0.12 +1.6 556,501 - Psest_0502 0.12 -0.3 556,501 - Psest_0502 0.12 +0.6 556,501 - Psest_0502 0.12 -0.8 556,608 + Psest_0502 0.18 -0.0 556,625 + Psest_0502 0.19 -1.0 556,626 - Psest_0502 0.19 +1.0 556,626 - Psest_0502 0.19 +1.0 556,626 - Psest_0502 0.19 +0.1 556,716 - Psest_0502 0.24 +1.6 556,752 + Psest_0502 0.27 -0.2 556,753 - Psest_0502 0.27 -3.0 556,905 - Psest_0502 0.36 -1.5 556,917 + Psest_0502 0.36 -1.9 556,972 + Psest_0502 0.40 -1.2 556,972 + Psest_0502 0.40 +3.4 556,972 + Psest_0502 0.40 -1.0 556,972 + Psest_0502 0.40 -1.6 556,972 + Psest_0502 0.40 -0.2 556,972 + Psest_0502 0.40 -1.2 556,972 + Psest_0502 0.40 -0.2 556,973 - Psest_0502 0.40 +0.0 556,973 - Psest_0502 0.40 +1.1 556,973 - Psest_0502 0.40 -2.1 556,974 + Psest_0502 0.40 +0.6 556,974 + Psest_0502 0.40 -1.3 556,974 + Psest_0502 0.40 -0.4 556,974 + Psest_0502 0.40 +0.9 556,974 + Psest_0502 0.40 +0.6 556,975 - Psest_0502 0.40 -0.3 556,975 - Psest_0502 0.40 -1.7 556,975 - Psest_0502 0.40 -0.6 557,193 + Psest_0502 0.53 -0.8 557,193 + Psest_0502 0.53 -0.0 557,193 + Psest_0502 0.53 +1.0 557,193 + Psest_0502 0.53 +2.0 557,193 + Psest_0502 0.53 +0.0 557,193 + Psest_0502 0.53 -0.8 557,193 + Psest_0502 0.53 -1.2 557,194 - Psest_0502 0.53 +0.4 557,194 - Psest_0502 0.53 +0.7 557,194 - Psest_0502 0.53 +0.7 557,194 - Psest_0502 0.53 +0.0 557,194 - Psest_0502 0.53 -1.3 557,194 - Psest_0502 0.53 +1.2 557,235 + Psest_0502 0.55 +0.6 557,262 + Psest_0502 0.57 -1.7 557,263 - Psest_0502 0.57 -1.1
Or see this region's nucleotide sequence