Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0487

Experiment: RCH2 grown on a colony of N2C3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0486 and Psest_0487 are separated by 183 nucleotidesPsest_0487 and Psest_0488 are separated by 104 nucleotides Psest_0486: Psest_0486 - DNA topoisomerase IV, A subunit, proteobacterial, at 535,982 to 538,234 _0486 Psest_0487: Psest_0487 - Uncharacterized protein conserved in bacteria, at 538,418 to 539,125 _0487 Psest_0488: Psest_0488 - Uncharacterized membrane protein affecting hemolysin expression, at 539,230 to 540,762 _0488 Position (kb) 538 539 540Strain fitness (log2 ratio) -2 -1 0 1 2at 538.408 kb on + strandat 538.408 kb on + strandat 538.408 kb on + strandat 538.409 kb on - strandat 538.505 kb on + strand, within Psest_0487at 538.506 kb on - strand, within Psest_0487at 538.522 kb on + strand, within Psest_0487at 538.589 kb on + strand, within Psest_0487at 538.589 kb on + strand, within Psest_0487at 538.589 kb on + strand, within Psest_0487at 538.589 kb on + strand, within Psest_0487at 538.589 kb on + strand, within Psest_0487at 538.932 kb on + strand, within Psest_0487at 538.933 kb on - strand, within Psest_0487at 538.933 kb on - strand, within Psest_0487at 538.933 kb on - strand, within Psest_0487at 538.948 kb on - strand, within Psest_0487

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3
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538,408 + +2.6
538,408 + +2.3
538,408 + -2.3
538,409 - -0.1
538,505 + Psest_0487 0.12 -1.4
538,506 - Psest_0487 0.12 -0.3
538,522 + Psest_0487 0.15 -0.8
538,589 + Psest_0487 0.24 -1.7
538,589 + Psest_0487 0.24 +1.0
538,589 + Psest_0487 0.24 -1.6
538,589 + Psest_0487 0.24 -1.1
538,589 + Psest_0487 0.24 -2.4
538,932 + Psest_0487 0.73 -1.3
538,933 - Psest_0487 0.73 -0.6
538,933 - Psest_0487 0.73 +0.6
538,933 - Psest_0487 0.73 +0.9
538,948 - Psest_0487 0.75 -2.5

Or see this region's nucleotide sequence