Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3822 and Psest_3823 are separated by 111 nucleotides Psest_3823 and Psest_3824 are separated by 31 nucleotides Psest_3824 and Psest_3825 are separated by 18 nucleotides
Psest_3822: Psest_3822 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 3,992,583 to 3,993,659
_3822
Psest_3823: Psest_3823 - hypothetical protein, at 3,993,771 to 3,995,048
_3823
Psest_3824: Psest_3824 - Predicted membrane protein, at 3,995,080 to 3,995,697
_3824
Psest_3825: Psest_3825 - Beta-propeller domains of methanol dehydrogenase type, at 3,995,716 to 3,996,468
_3825
Position (kb)
3993
3994
3995
3996 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3992.804 kb on + strand, within Psest_3822 at 3992.804 kb on + strand, within Psest_3822 at 3992.805 kb on - strand, within Psest_3822 at 3992.812 kb on + strand, within Psest_3822 at 3992.812 kb on + strand, within Psest_3822 at 3992.812 kb on + strand, within Psest_3822 at 3992.812 kb on + strand, within Psest_3822 at 3992.812 kb on + strand, within Psest_3822 at 3992.812 kb on + strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.813 kb on - strand, within Psest_3822 at 3992.917 kb on + strand, within Psest_3822 at 3993.454 kb on + strand, within Psest_3822 at 3993.454 kb on + strand, within Psest_3822 at 3993.454 kb on + strand, within Psest_3822 at 3993.455 kb on - strand, within Psest_3822 at 3993.455 kb on - strand, within Psest_3822 at 3993.455 kb on - strand, within Psest_3822 at 3993.593 kb on + strand at 3993.720 kb on + strand at 3993.720 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.764 kb on + strand at 3993.765 kb on - strand at 3993.765 kb on - strand at 3993.765 kb on - strand at 3993.765 kb on - strand at 3993.765 kb on - strand at 3993.765 kb on - strand at 3993.946 kb on - strand, within Psest_3823 at 3993.993 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.680 kb on + strand, within Psest_3823 at 3994.681 kb on - strand, within Psest_3823 at 3994.681 kb on - strand, within Psest_3823 at 3994.681 kb on - strand, within Psest_3823 at 3995.020 kb on + strand at 3995.020 kb on + strand at 3995.020 kb on + strand at 3995.020 kb on + strand at 3995.020 kb on + strand at 3995.021 kb on - strand at 3995.021 kb on - strand at 3995.267 kb on - strand, within Psest_3824 at 3995.294 kb on + strand, within Psest_3824 at 3995.294 kb on + strand, within Psest_3824 at 3995.294 kb on + strand, within Psest_3824 at 3995.294 kb on + strand, within Psest_3824 at 3995.295 kb on - strand, within Psest_3824 at 3995.404 kb on + strand, within Psest_3824 at 3995.579 kb on + strand, within Psest_3824 at 3995.579 kb on + strand, within Psest_3824 at 3995.580 kb on - strand, within Psest_3824 at 3995.580 kb on - strand, within Psest_3824 at 3995.615 kb on - strand, within Psest_3824 at 3995.689 kb on + strand at 3995.689 kb on + strand at 3995.718 kb on - strand at 3995.718 kb on - strand at 3995.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 3,992,804 + Psest_3822 0.21 +0.8 3,992,804 + Psest_3822 0.21 +0.5 3,992,805 - Psest_3822 0.21 +2.3 3,992,812 + Psest_3822 0.21 +1.2 3,992,812 + Psest_3822 0.21 -2.8 3,992,812 + Psest_3822 0.21 -2.9 3,992,812 + Psest_3822 0.21 +1.0 3,992,812 + Psest_3822 0.21 -1.2 3,992,812 + Psest_3822 0.21 +1.0 3,992,813 - Psest_3822 0.21 -1.6 3,992,813 - Psest_3822 0.21 +1.0 3,992,813 - Psest_3822 0.21 +0.6 3,992,813 - Psest_3822 0.21 -1.6 3,992,813 - Psest_3822 0.21 -2.5 3,992,813 - Psest_3822 0.21 +2.2 3,992,813 - Psest_3822 0.21 -2.8 3,992,813 - Psest_3822 0.21 +2.0 3,992,813 - Psest_3822 0.21 -2.4 3,992,813 - Psest_3822 0.21 -0.1 3,992,813 - Psest_3822 0.21 -1.0 3,992,813 - Psest_3822 0.21 +2.6 3,992,917 + Psest_3822 0.31 -1.4 3,993,454 + Psest_3822 0.81 +1.7 3,993,454 + Psest_3822 0.81 +0.0 3,993,454 + Psest_3822 0.81 -1.4 3,993,455 - Psest_3822 0.81 +0.3 3,993,455 - Psest_3822 0.81 +1.6 3,993,455 - Psest_3822 0.81 +1.0 3,993,593 + -0.2 3,993,720 + -2.4 3,993,720 + -1.1 3,993,764 + +0.2 3,993,764 + -1.0 3,993,764 + +1.0 3,993,764 + -2.1 3,993,764 + +0.6 3,993,764 + +2.1 3,993,764 + -0.5 3,993,764 + +0.8 3,993,764 + -2.8 3,993,764 + +1.4 3,993,764 + -0.4 3,993,764 + +0.3 3,993,765 - -1.1 3,993,765 - -2.6 3,993,765 - +0.3 3,993,765 - -0.7 3,993,765 - -0.0 3,993,765 - +0.4 3,993,946 - Psest_3823 0.14 -2.1 3,993,993 + Psest_3823 0.17 +0.4 3,994,680 + Psest_3823 0.71 -1.1 3,994,680 + Psest_3823 0.71 -1.1 3,994,680 + Psest_3823 0.71 -2.7 3,994,680 + Psest_3823 0.71 -1.0 3,994,680 + Psest_3823 0.71 -0.5 3,994,680 + Psest_3823 0.71 -1.8 3,994,680 + Psest_3823 0.71 -1.7 3,994,680 + Psest_3823 0.71 +0.3 3,994,680 + Psest_3823 0.71 -1.4 3,994,681 - Psest_3823 0.71 -2.5 3,994,681 - Psest_3823 0.71 -0.1 3,994,681 - Psest_3823 0.71 +1.0 3,995,020 + +1.5 3,995,020 + +1.0 3,995,020 + -1.2 3,995,020 + -1.9 3,995,020 + +1.3 3,995,021 - -0.2 3,995,021 - -1.9 3,995,267 - Psest_3824 0.30 -1.7 3,995,294 + Psest_3824 0.35 +1.5 3,995,294 + Psest_3824 0.35 -2.1 3,995,294 + Psest_3824 0.35 -0.3 3,995,294 + Psest_3824 0.35 -0.2 3,995,295 - Psest_3824 0.35 -0.2 3,995,404 + Psest_3824 0.52 -0.2 3,995,579 + Psest_3824 0.81 -0.8 3,995,579 + Psest_3824 0.81 +0.5 3,995,580 - Psest_3824 0.81 -1.8 3,995,580 - Psest_3824 0.81 -1.3 3,995,615 - Psest_3824 0.87 +1.3 3,995,689 + -1.5 3,995,689 + -0.2 3,995,718 - +0.8 3,995,718 - -0.1 3,995,718 - +0.3
Or see this region's nucleotide sequence