Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3495 and Psest_3496 are separated by 117 nucleotides Psest_3496 and Psest_3497 are separated by 129 nucleotides Psest_3497 and Psest_3498 are separated by 105 nucleotides Psest_3498 and Psest_3499 overlap by 4 nucleotides
Psest_3495: Psest_3495 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 3,668,285 to 3,669,028
_3495
Psest_3496: Psest_3496 - Predicted metal-binding protein, at 3,669,146 to 3,669,580
_3496
Psest_3497: Psest_3497 - Predicted metalloprotease, at 3,669,710 to 3,670,567
_3497
Psest_3498: Psest_3498 - haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED/haloacid dehalogenase superfamily, subfamily IA, variant 1 with third motif having Dx(3-4)D or Dx(3-4)E, at 3,670,673 to 3,671,275
_3498
Psest_3499: Psest_3499 - acyl-CoA thioesterase II, at 3,671,272 to 3,672,141
_3499
Position (kb)
3669
3670
3671 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3668.811 kb on + strand, within Psest_3495 at 3668.811 kb on + strand, within Psest_3495 at 3668.811 kb on + strand, within Psest_3495 at 3668.811 kb on + strand, within Psest_3495 at 3668.812 kb on - strand, within Psest_3495 at 3669.145 kb on + strand at 3669.145 kb on + strand at 3669.145 kb on + strand at 3669.146 kb on - strand at 3669.426 kb on + strand, within Psest_3496 at 3669.426 kb on + strand, within Psest_3496 at 3669.427 kb on - strand, within Psest_3496 at 3669.744 kb on + strand at 3669.745 kb on - strand at 3669.809 kb on - strand, within Psest_3497 at 3670.088 kb on + strand, within Psest_3497 at 3670.088 kb on + strand, within Psest_3497 at 3670.089 kb on - strand, within Psest_3497 at 3670.181 kb on + strand, within Psest_3497 at 3670.181 kb on + strand, within Psest_3497 at 3670.181 kb on + strand, within Psest_3497 at 3670.181 kb on + strand, within Psest_3497 at 3670.182 kb on - strand at 3670.182 kb on - strand, within Psest_3497 at 3670.182 kb on - strand, within Psest_3497 at 3670.182 kb on - strand, within Psest_3497 at 3670.565 kb on + strand at 3670.629 kb on - strand at 3670.726 kb on - strand at 3670.726 kb on - strand at 3671.077 kb on - strand, within Psest_3498 at 3671.402 kb on + strand, within Psest_3499 at 3671.402 kb on + strand, within Psest_3499 at 3671.402 kb on + strand, within Psest_3499 at 3671.402 kb on + strand, within Psest_3499 at 3671.540 kb on + strand, within Psest_3499 at 3671.552 kb on + strand, within Psest_3499 at 3671.552 kb on + strand, within Psest_3499 at 3671.552 kb on + strand, within Psest_3499 at 3671.552 kb on + strand, within Psest_3499 at 3671.552 kb on + strand, within Psest_3499 at 3671.553 kb on - strand, within Psest_3499 at 3671.553 kb on - strand, within Psest_3499 at 3671.553 kb on - strand, within Psest_3499 at 3671.553 kb on - strand, within Psest_3499
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 3,668,811 + Psest_3495 0.71 +1.1 3,668,811 + Psest_3495 0.71 -0.4 3,668,811 + Psest_3495 0.71 -0.5 3,668,811 + Psest_3495 0.71 -2.1 3,668,812 - Psest_3495 0.71 -0.2 3,669,145 + +0.5 3,669,145 + +0.1 3,669,145 + -0.4 3,669,146 - -0.7 3,669,426 + Psest_3496 0.64 -2.1 3,669,426 + Psest_3496 0.64 +0.5 3,669,427 - Psest_3496 0.65 -1.3 3,669,744 + -1.5 3,669,745 - -0.2 3,669,809 - Psest_3497 0.12 -0.3 3,670,088 + Psest_3497 0.44 -1.7 3,670,088 + Psest_3497 0.44 -0.4 3,670,089 - Psest_3497 0.44 -1.9 3,670,181 + Psest_3497 0.55 -0.7 3,670,181 + Psest_3497 0.55 +1.8 3,670,181 + Psest_3497 0.55 -0.4 3,670,181 + Psest_3497 0.55 -1.1 3,670,182 - -0.3 3,670,182 - Psest_3497 0.55 -2.7 3,670,182 - Psest_3497 0.55 -1.2 3,670,182 - Psest_3497 0.55 -1.4 3,670,565 + -0.1 3,670,629 - +0.3 3,670,726 - +0.5 3,670,726 - +2.9 3,671,077 - Psest_3498 0.67 +1.1 3,671,402 + Psest_3499 0.15 -1.6 3,671,402 + Psest_3499 0.15 +0.6 3,671,402 + Psest_3499 0.15 -0.1 3,671,402 + Psest_3499 0.15 +0.9 3,671,540 + Psest_3499 0.31 -1.3 3,671,552 + Psest_3499 0.32 +1.3 3,671,552 + Psest_3499 0.32 +0.4 3,671,552 + Psest_3499 0.32 -1.0 3,671,552 + Psest_3499 0.32 -0.2 3,671,552 + Psest_3499 0.32 -0.2 3,671,553 - Psest_3499 0.32 -0.9 3,671,553 - Psest_3499 0.32 -0.3 3,671,553 - Psest_3499 0.32 -1.5 3,671,553 - Psest_3499 0.32 +0.1
Or see this region's nucleotide sequence