Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3475 and Psest_3476 are separated by 50 nucleotides Psest_3476 and Psest_3477 overlap by 7 nucleotides Psest_3477 and Psest_3478 are separated by 88 nucleotides Psest_3478 and Psest_3479 are separated by 55 nucleotides
Psest_3475: Psest_3475 - hypothetical protein, at 3,644,716 to 3,645,366
_3475
Psest_3476: Psest_3476 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 3,645,417 to 3,646,076
_3476
Psest_3477: Psest_3477 - Signal transduction histidine kinase, at 3,646,070 to 3,647,509
_3477
Psest_3478: Psest_3478 - Cytochrome B561, at 3,647,598 to 3,648,149
_3478
Psest_3479: Psest_3479 - molybdenum cofactor synthesis domain, at 3,648,205 to 3,649,437
_3479
Position (kb)
3646
3647
3648 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3645.074 kb on - strand, within Psest_3475 at 3645.075 kb on + strand, within Psest_3475 at 3645.094 kb on + strand, within Psest_3475 at 3645.095 kb on - strand, within Psest_3475 at 3645.095 kb on - strand, within Psest_3475 at 3645.095 kb on - strand, within Psest_3475 at 3645.199 kb on + strand, within Psest_3475 at 3645.199 kb on + strand, within Psest_3475 at 3645.199 kb on + strand, within Psest_3475 at 3645.199 kb on + strand, within Psest_3475 at 3645.199 kb on + strand, within Psest_3475 at 3645.199 kb on + strand, within Psest_3475 at 3645.199 kb on + strand, within Psest_3475 at 3645.200 kb on - strand, within Psest_3475 at 3645.200 kb on - strand, within Psest_3475 at 3645.236 kb on - strand, within Psest_3475 at 3645.844 kb on - strand, within Psest_3476 at 3645.951 kb on + strand, within Psest_3476 at 3646.334 kb on + strand, within Psest_3477 at 3646.361 kb on + strand, within Psest_3477 at 3646.361 kb on + strand, within Psest_3477 at 3646.361 kb on + strand, within Psest_3477 at 3646.362 kb on - strand, within Psest_3477 at 3646.362 kb on - strand, within Psest_3477 at 3647.223 kb on - strand, within Psest_3477 at 3647.223 kb on - strand, within Psest_3477 at 3647.507 kb on + strand at 3647.507 kb on + strand at 3647.507 kb on + strand at 3647.508 kb on - strand at 3647.547 kb on + strand at 3647.547 kb on + strand at 3647.561 kb on + strand at 3647.561 kb on + strand at 3647.561 kb on + strand at 3647.561 kb on + strand at 3647.561 kb on + strand at 3647.561 kb on + strand at 3647.562 kb on - strand at 3647.562 kb on - strand at 3647.562 kb on - strand at 3647.562 kb on - strand at 3647.562 kb on - strand at 3647.562 kb on - strand at 3647.590 kb on + strand at 3647.591 kb on - strand at 3647.626 kb on - strand at 3647.626 kb on - strand at 3647.886 kb on + strand, within Psest_3478 at 3647.887 kb on - strand, within Psest_3478 at 3648.094 kb on - strand at 3648.094 kb on - strand at 3648.233 kb on + strand at 3648.233 kb on + strand at 3648.233 kb on + strand at 3648.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 3,645,074 - Psest_3475 0.55 +2.4 3,645,075 + Psest_3475 0.55 -1.7 3,645,094 + Psest_3475 0.58 -2.2 3,645,095 - Psest_3475 0.58 +1.6 3,645,095 - Psest_3475 0.58 +1.8 3,645,095 - Psest_3475 0.58 -3.0 3,645,199 + Psest_3475 0.74 +2.1 3,645,199 + Psest_3475 0.74 -2.3 3,645,199 + Psest_3475 0.74 +0.9 3,645,199 + Psest_3475 0.74 -1.2 3,645,199 + Psest_3475 0.74 -1.8 3,645,199 + Psest_3475 0.74 -1.5 3,645,199 + Psest_3475 0.74 -0.5 3,645,200 - Psest_3475 0.74 +0.7 3,645,200 - Psest_3475 0.74 -1.8 3,645,236 - Psest_3475 0.80 +1.1 3,645,844 - Psest_3476 0.65 -0.2 3,645,951 + Psest_3476 0.81 -0.4 3,646,334 + Psest_3477 0.18 -1.2 3,646,361 + Psest_3477 0.20 -2.8 3,646,361 + Psest_3477 0.20 -0.9 3,646,361 + Psest_3477 0.20 -0.3 3,646,362 - Psest_3477 0.20 -3.0 3,646,362 - Psest_3477 0.20 +0.2 3,647,223 - Psest_3477 0.80 -0.6 3,647,223 - Psest_3477 0.80 -0.0 3,647,507 + +0.9 3,647,507 + -1.6 3,647,507 + +0.5 3,647,508 - +0.1 3,647,547 + -2.4 3,647,547 + -0.1 3,647,561 + -0.9 3,647,561 + +0.2 3,647,561 + -1.9 3,647,561 + -0.6 3,647,561 + +2.3 3,647,561 + -0.3 3,647,562 - -1.4 3,647,562 - +0.1 3,647,562 - +1.2 3,647,562 - -0.7 3,647,562 - -1.9 3,647,562 - +0.3 3,647,590 + +0.4 3,647,591 - -0.6 3,647,626 - +0.4 3,647,626 - -2.0 3,647,886 + Psest_3478 0.52 -0.7 3,647,887 - Psest_3478 0.52 +0.6 3,648,094 - -0.9 3,648,094 - -3.3 3,648,233 + -1.2 3,648,233 + +0.9 3,648,233 + -0.9 3,648,234 - -0.1
Or see this region's nucleotide sequence