Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2714 and Psest_2715 are separated by 93 nucleotides Psest_2715 and Psest_2716 overlap by 4 nucleotides Psest_2716 and Psest_2717 are separated by 85 nucleotides
Psest_2714: Psest_2714 - 7-cyano-7-deazaguanine reductase, at 2,903,667 to 2,904,497
_2714
Psest_2715: Psest_2715 - ABC-type polysaccharide/polyol phosphate export systems, permease component, at 2,904,591 to 2,905,370
_2715
Psest_2716: Psest_2716 - ABC-type multidrug transport system, ATPase component, at 2,905,367 to 2,906,296
_2716
Psest_2717: Psest_2717 - Succinylglutamate desuccinylase, at 2,906,382 to 2,907,380
_2717
Position (kb)
2905
2906
2907 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2904.440 kb on - strand at 2904.440 kb on - strand at 2904.640 kb on + strand at 2904.640 kb on + strand at 2904.687 kb on - strand, within Psest_2715 at 2904.797 kb on - strand, within Psest_2715 at 2904.797 kb on - strand, within Psest_2715 at 2904.797 kb on - strand, within Psest_2715 at 2904.797 kb on - strand, within Psest_2715 at 2905.088 kb on + strand, within Psest_2715 at 2905.180 kb on + strand, within Psest_2715 at 2905.180 kb on + strand, within Psest_2715 at 2905.180 kb on + strand, within Psest_2715 at 2905.181 kb on - strand, within Psest_2715 at 2905.181 kb on - strand, within Psest_2715 at 2905.181 kb on - strand, within Psest_2715 at 2905.181 kb on - strand, within Psest_2715 at 2905.181 kb on - strand, within Psest_2715 at 2905.181 kb on - strand, within Psest_2715 at 2905.181 kb on - strand, within Psest_2715 at 2905.189 kb on + strand, within Psest_2715 at 2905.190 kb on - strand, within Psest_2715 at 2905.190 kb on - strand, within Psest_2715 at 2905.250 kb on - strand, within Psest_2715 at 2905.250 kb on - strand, within Psest_2715 at 2905.250 kb on - strand, within Psest_2715 at 2905.250 kb on - strand, within Psest_2715 at 2905.304 kb on - strand at 2905.509 kb on - strand, within Psest_2716 at 2905.555 kb on + strand, within Psest_2716 at 2905.556 kb on - strand, within Psest_2716 at 2906.256 kb on + strand at 2906.256 kb on + strand at 2906.256 kb on + strand at 2906.256 kb on + strand at 2906.257 kb on - strand at 2906.257 kb on - strand at 2906.257 kb on - strand at 2906.257 kb on - strand at 2906.257 kb on - strand at 2906.257 kb on - strand at 2906.291 kb on - strand at 2906.296 kb on + strand at 2906.330 kb on + strand at 2906.330 kb on + strand at 2906.330 kb on + strand at 2906.331 kb on - strand at 2906.331 kb on - strand at 2906.335 kb on - strand at 2906.420 kb on + strand at 2906.421 kb on - strand at 2906.421 kb on - strand at 2906.938 kb on + strand, within Psest_2717 at 2906.939 kb on - strand, within Psest_2717 at 2907.024 kb on + strand, within Psest_2717 at 2907.024 kb on + strand, within Psest_2717 at 2907.024 kb on + strand, within Psest_2717 at 2907.025 kb on - strand, within Psest_2717 at 2907.025 kb on - strand, within Psest_2717 at 2907.025 kb on - strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.183 kb on + strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717 at 2907.184 kb on - strand, within Psest_2717
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 2,904,440 - -0.1 2,904,440 - +0.9 2,904,640 + +0.3 2,904,640 + +1.0 2,904,687 - Psest_2715 0.12 -2.7 2,904,797 - Psest_2715 0.26 +0.3 2,904,797 - Psest_2715 0.26 -1.9 2,904,797 - Psest_2715 0.26 +0.5 2,904,797 - Psest_2715 0.26 -0.4 2,905,088 + Psest_2715 0.64 -1.0 2,905,180 + Psest_2715 0.76 -0.5 2,905,180 + Psest_2715 0.76 -0.6 2,905,180 + Psest_2715 0.76 -3.4 2,905,181 - Psest_2715 0.76 -0.3 2,905,181 - Psest_2715 0.76 +0.5 2,905,181 - Psest_2715 0.76 +0.8 2,905,181 - Psest_2715 0.76 -0.9 2,905,181 - Psest_2715 0.76 +1.6 2,905,181 - Psest_2715 0.76 +0.2 2,905,181 - Psest_2715 0.76 -0.6 2,905,189 + Psest_2715 0.77 +0.8 2,905,190 - Psest_2715 0.77 +0.0 2,905,190 - Psest_2715 0.77 +0.5 2,905,250 - Psest_2715 0.84 +1.3 2,905,250 - Psest_2715 0.84 -0.5 2,905,250 - Psest_2715 0.84 +0.4 2,905,250 - Psest_2715 0.84 +0.5 2,905,304 - +2.7 2,905,509 - Psest_2716 0.15 -1.4 2,905,555 + Psest_2716 0.20 -1.8 2,905,556 - Psest_2716 0.20 -0.2 2,906,256 + +2.0 2,906,256 + +0.1 2,906,256 + +2.5 2,906,256 + +0.6 2,906,257 - -1.9 2,906,257 - +0.1 2,906,257 - +1.7 2,906,257 - -1.3 2,906,257 - -2.0 2,906,257 - -1.5 2,906,291 - -0.5 2,906,296 + +0.9 2,906,330 + +1.1 2,906,330 + +0.9 2,906,330 + +1.0 2,906,331 - +1.4 2,906,331 - +0.5 2,906,335 - +0.0 2,906,420 + +1.7 2,906,421 - -2.2 2,906,421 - -0.8 2,906,938 + Psest_2717 0.56 +0.0 2,906,939 - Psest_2717 0.56 +1.5 2,907,024 + Psest_2717 0.64 -0.9 2,907,024 + Psest_2717 0.64 +1.7 2,907,024 + Psest_2717 0.64 -2.2 2,907,025 - Psest_2717 0.64 -0.6 2,907,025 - Psest_2717 0.64 +0.2 2,907,025 - Psest_2717 0.64 -1.3 2,907,183 + Psest_2717 0.80 -2.0 2,907,183 + Psest_2717 0.80 -0.5 2,907,183 + Psest_2717 0.80 -1.6 2,907,183 + Psest_2717 0.80 -0.3 2,907,183 + Psest_2717 0.80 -0.2 2,907,183 + Psest_2717 0.80 -1.4 2,907,183 + Psest_2717 0.80 +1.6 2,907,183 + Psest_2717 0.80 -0.6 2,907,183 + Psest_2717 0.80 +0.3 2,907,184 - Psest_2717 0.80 +0.2 2,907,184 - Psest_2717 0.80 -1.1 2,907,184 - Psest_2717 0.80 -0.1 2,907,184 - Psest_2717 0.80 +1.7 2,907,184 - Psest_2717 0.80 +0.1 2,907,184 - Psest_2717 0.80 -0.8 2,907,184 - Psest_2717 0.80 +1.2 2,907,184 - Psest_2717 0.80 -0.1
Or see this region's nucleotide sequence