Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0247 and Psest_0248 are separated by 129 nucleotides Psest_0248 and Psest_0249 are separated by 175 nucleotides Psest_0249 and Psest_0250 overlap by 4 nucleotides
Psest_0247: Psest_0247 - NAD-dependent aldehyde dehydrogenases, at 257,716 to 259,128
_0247
Psest_0248: Psest_0248 - Transcriptional regulator, at 259,258 to 259,902
_0248
Psest_0249: Psest_0249 - UDP-N-acetylglucosamine 2-epimerase, at 260,078 to 261,268
_0249
Psest_0250: Psest_0250 - Glycosyltransferases, probably involved in cell wall biogenesis, at 261,265 to 263,451
_0250
Position (kb) 260
261
262 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 259.163 kb on - strand at 259.198 kb on + strand at 259.198 kb on + strand at 259.198 kb on + strand at 259.198 kb on + strand at 259.216 kb on + strand at 259.216 kb on + strand at 259.217 kb on - strand at 259.217 kb on - strand at 259.387 kb on + strand, within Psest_0248 at 259.387 kb on + strand, within Psest_0248 at 259.387 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.390 kb on + strand, within Psest_0248 at 259.391 kb on - strand, within Psest_0248 at 259.438 kb on + strand, within Psest_0248 at 259.510 kb on + strand, within Psest_0248 at 259.510 kb on + strand, within Psest_0248 at 259.511 kb on - strand, within Psest_0248 at 259.511 kb on - strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.651 kb on + strand, within Psest_0248 at 259.652 kb on - strand, within Psest_0248 at 259.715 kb on + strand, within Psest_0248 at 259.715 kb on + strand, within Psest_0248 at 259.715 kb on + strand, within Psest_0248 at 259.716 kb on - strand, within Psest_0248 at 259.807 kb on + strand, within Psest_0248 at 259.807 kb on + strand, within Psest_0248 at 259.807 kb on + strand, within Psest_0248 at 259.808 kb on - strand, within Psest_0248 at 259.862 kb on + strand at 259.863 kb on - strand at 259.863 kb on - strand at 259.863 kb on - strand at 259.863 kb on - strand at 259.868 kb on + strand at 259.869 kb on - strand at 259.869 kb on - strand at 259.869 kb on - strand at 259.936 kb on - strand at 260.105 kb on - strand at 260.173 kb on + strand at 260.356 kb on - strand, within Psest_0249 at 260.376 kb on + strand, within Psest_0249 at 260.376 kb on + strand, within Psest_0249 at 260.376 kb on + strand, within Psest_0249 at 260.377 kb on - strand, within Psest_0249 at 260.377 kb on - strand, within Psest_0249 at 260.472 kb on + strand, within Psest_0249 at 260.510 kb on + strand, within Psest_0249 at 260.677 kb on + strand, within Psest_0249 at 260.677 kb on + strand, within Psest_0249 at 260.677 kb on + strand, within Psest_0249 at 260.678 kb on - strand, within Psest_0249 at 260.678 kb on - strand, within Psest_0249 at 260.862 kb on + strand, within Psest_0249 at 260.862 kb on + strand, within Psest_0249 at 260.863 kb on - strand, within Psest_0249 at 260.894 kb on + strand, within Psest_0249 at 260.960 kb on + strand, within Psest_0249 at 261.045 kb on + strand, within Psest_0249 at 261.106 kb on + strand, within Psest_0249 at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.221 kb on - strand at 261.248 kb on - strand at 261.277 kb on + strand at 261.277 kb on + strand at 261.277 kb on + strand at 261.278 kb on - strand at 261.278 kb on - strand at 261.278 kb on - strand at 261.283 kb on + strand at 261.283 kb on + strand at 261.283 kb on + strand at 261.284 kb on - strand at 261.284 kb on - strand at 261.284 kb on - strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.307 kb on + strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.308 kb on - strand at 261.310 kb on + strand at 261.310 kb on + strand at 261.311 kb on - strand at 261.311 kb on - strand at 261.311 kb on - strand at 261.311 kb on - strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.316 kb on + strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.317 kb on - strand at 261.469 kb on + strand at 261.469 kb on + strand at 261.470 kb on - strand at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.527 kb on + strand, within Psest_0250 at 261.528 kb on - strand, within Psest_0250 at 261.528 kb on - strand, within Psest_0250 at 261.528 kb on - strand, within Psest_0250 at 261.568 kb on + strand, within Psest_0250 at 261.568 kb on + strand, within Psest_0250 at 261.569 kb on - strand, within Psest_0250 at 261.578 kb on - strand, within Psest_0250 at 261.584 kb on + strand, within Psest_0250 at 261.584 kb on + strand, within Psest_0250 at 261.585 kb on - strand, within Psest_0250 at 261.594 kb on + strand, within Psest_0250 at 261.594 kb on + strand, within Psest_0250 at 261.594 kb on + strand, within Psest_0250 at 261.595 kb on - strand, within Psest_0250 at 261.595 kb on - strand, within Psest_0250 at 261.595 kb on - strand, within Psest_0250 at 261.829 kb on + strand, within Psest_0250 at 261.829 kb on + strand, within Psest_0250 at 261.829 kb on + strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.830 kb on - strand, within Psest_0250 at 261.836 kb on + strand, within Psest_0250 at 261.869 kb on - strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.874 kb on + strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 261.875 kb on - strand, within Psest_0250 at 262.061 kb on + strand, within Psest_0250 at 262.061 kb on + strand, within Psest_0250 at 262.082 kb on - strand, within Psest_0250 at 262.082 kb on - strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.124 kb on + strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.125 kb on - strand, within Psest_0250 at 262.208 kb on + strand, within Psest_0250 at 262.218 kb on - strand, within Psest_0250
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 259,163 - +1.0 259,198 + +2.2 259,198 + +1.0 259,198 + -1.0 259,198 + +0.0 259,216 + +1.2 259,216 + -0.1 259,217 - +1.5 259,217 - +1.5 259,387 + Psest_0248 0.20 +0.9 259,387 + Psest_0248 0.20 +1.5 259,387 + Psest_0248 0.20 -0.2 259,390 + Psest_0248 0.20 +0.0 259,390 + Psest_0248 0.20 +1.6 259,390 + Psest_0248 0.20 -0.9 259,390 + Psest_0248 0.20 +0.3 259,390 + Psest_0248 0.20 +0.5 259,391 - Psest_0248 0.21 -2.3 259,438 + Psest_0248 0.28 -0.4 259,510 + Psest_0248 0.39 -2.2 259,510 + Psest_0248 0.39 -1.0 259,511 - Psest_0248 0.39 +0.3 259,511 - Psest_0248 0.39 +0.1 259,651 + Psest_0248 0.61 +1.2 259,651 + Psest_0248 0.61 -0.3 259,651 + Psest_0248 0.61 -0.9 259,651 + Psest_0248 0.61 -2.3 259,651 + Psest_0248 0.61 +1.1 259,652 - Psest_0248 0.61 +0.8 259,715 + Psest_0248 0.71 +1.7 259,715 + Psest_0248 0.71 -2.8 259,715 + Psest_0248 0.71 -1.8 259,716 - Psest_0248 0.71 +1.4 259,807 + Psest_0248 0.85 -3.0 259,807 + Psest_0248 0.85 -0.9 259,807 + Psest_0248 0.85 +1.0 259,808 - Psest_0248 0.85 +2.1 259,862 + +1.0 259,863 - +1.4 259,863 - -1.3 259,863 - +0.9 259,863 - +1.5 259,868 + +0.7 259,869 - -1.6 259,869 - -0.4 259,869 - -1.3 259,936 - +0.2 260,105 - -0.2 260,173 + -2.3 260,356 - Psest_0249 0.23 +1.7 260,376 + Psest_0249 0.25 -2.2 260,376 + Psest_0249 0.25 -1.8 260,376 + Psest_0249 0.25 -1.4 260,377 - Psest_0249 0.25 +0.2 260,377 - Psest_0249 0.25 +0.6 260,472 + Psest_0249 0.33 -1.9 260,510 + Psest_0249 0.36 +0.6 260,677 + Psest_0249 0.50 -0.5 260,677 + Psest_0249 0.50 -2.5 260,677 + Psest_0249 0.50 -0.3 260,678 - Psest_0249 0.50 -1.2 260,678 - Psest_0249 0.50 -2.4 260,862 + Psest_0249 0.66 -3.0 260,862 + Psest_0249 0.66 -1.6 260,863 - Psest_0249 0.66 -2.5 260,894 + Psest_0249 0.69 +1.7 260,960 + Psest_0249 0.74 -0.3 261,045 + Psest_0249 0.81 +0.6 261,106 + Psest_0249 0.86 +0.6 261,220 + -2.2 261,220 + -0.8 261,220 + -2.5 261,220 + +0.3 261,220 + -0.5 261,220 + +3.9 261,221 - -1.0 261,221 - -1.0 261,221 - +1.4 261,221 - -1.8 261,221 - +0.6 261,221 - -3.0 261,221 - +0.1 261,221 - +1.3 261,221 - +1.7 261,221 - +3.9 261,221 - -0.7 261,248 - +1.4 261,277 + +2.5 261,277 + -0.3 261,277 + -1.4 261,278 - -1.4 261,278 - -2.3 261,278 - +0.3 261,283 + -0.9 261,283 + +3.6 261,283 + +1.6 261,284 - +1.7 261,284 - +0.1 261,284 - -1.1 261,307 + +1.1 261,307 + -1.7 261,307 + +0.3 261,307 + +4.2 261,307 + -0.5 261,308 - +0.6 261,308 - +0.6 261,308 - -1.9 261,308 - +0.6 261,308 - +0.4 261,310 + +0.3 261,310 + -0.6 261,311 - +2.5 261,311 - +1.6 261,311 - +0.3 261,311 - -2.4 261,316 + +1.1 261,316 + +0.6 261,316 + -0.2 261,316 + -2.0 261,316 + -0.3 261,316 + -0.6 261,317 - +0.4 261,317 - +1.2 261,317 - -0.5 261,317 - -0.6 261,317 - -2.3 261,317 - +0.0 261,469 + +0.3 261,469 + -0.7 261,470 - -1.2 261,527 + Psest_0250 0.12 +0.7 261,527 + Psest_0250 0.12 -1.4 261,527 + Psest_0250 0.12 +1.7 261,527 + Psest_0250 0.12 +0.6 261,527 + Psest_0250 0.12 -1.4 261,527 + Psest_0250 0.12 -2.2 261,528 - Psest_0250 0.12 +0.6 261,528 - Psest_0250 0.12 +3.9 261,528 - Psest_0250 0.12 -0.4 261,568 + Psest_0250 0.14 -0.3 261,568 + Psest_0250 0.14 -0.0 261,569 - Psest_0250 0.14 -0.1 261,578 - Psest_0250 0.14 +1.6 261,584 + Psest_0250 0.15 -0.3 261,584 + Psest_0250 0.15 +2.3 261,585 - Psest_0250 0.15 +0.7 261,594 + Psest_0250 0.15 +0.6 261,594 + Psest_0250 0.15 +2.0 261,594 + Psest_0250 0.15 +1.1 261,595 - Psest_0250 0.15 -0.7 261,595 - Psest_0250 0.15 -1.7 261,595 - Psest_0250 0.15 +0.6 261,829 + Psest_0250 0.26 -0.1 261,829 + Psest_0250 0.26 +2.4 261,829 + Psest_0250 0.26 +1.3 261,830 - Psest_0250 0.26 +0.6 261,830 - Psest_0250 0.26 -1.9 261,830 - Psest_0250 0.26 +1.0 261,830 - Psest_0250 0.26 +0.1 261,836 + Psest_0250 0.26 -0.0 261,869 - Psest_0250 0.28 -2.1 261,874 + Psest_0250 0.28 -1.7 261,874 + Psest_0250 0.28 -0.1 261,874 + Psest_0250 0.28 -2.2 261,874 + Psest_0250 0.28 +0.5 261,874 + Psest_0250 0.28 +1.0 261,874 + Psest_0250 0.28 +0.5 261,874 + Psest_0250 0.28 -2.4 261,874 + Psest_0250 0.28 -0.6 261,874 + Psest_0250 0.28 -0.7 261,874 + Psest_0250 0.28 -0.0 261,875 - Psest_0250 0.28 +1.6 261,875 - Psest_0250 0.28 -2.4 261,875 - Psest_0250 0.28 +2.8 261,875 - Psest_0250 0.28 +0.5 262,061 + Psest_0250 0.36 +1.5 262,061 + Psest_0250 0.36 -0.7 262,082 - Psest_0250 0.37 +0.9 262,082 - Psest_0250 0.37 +1.9 262,124 + Psest_0250 0.39 -2.3 262,124 + Psest_0250 0.39 +0.4 262,124 + Psest_0250 0.39 +1.0 262,124 + Psest_0250 0.39 -1.3 262,125 - Psest_0250 0.39 +1.4 262,125 - Psest_0250 0.39 -0.7 262,125 - Psest_0250 0.39 +0.7 262,125 - Psest_0250 0.39 -0.7 262,125 - Psest_0250 0.39 +3.2 262,208 + Psest_0250 0.43 +0.2 262,218 - Psest_0250 0.44 -2.7
Or see this region's nucleotide sequence