Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2176 and Psest_2177 are separated by 11 nucleotides Psest_2177 and Psest_2178 are separated by 270 nucleotides Psest_2178 and Psest_2179 are separated by 30 nucleotides Psest_2179 and Psest_2180 are separated by 163 nucleotides
Psest_2176: Psest_2176 - Predicted membrane protein, at 2,333,122 to 2,333,406
_2176
Psest_2177: Psest_2177 - Protein of unknown function (DUF1652)., at 2,333,418 to 2,333,693
_2177
Psest_2178: Psest_2178 - hypothetical protein, at 2,333,964 to 2,334,260
_2178
Psest_2179: Psest_2179 - DNA ligase D, at 2,334,291 to 2,336,855
_2179
Psest_2180: Psest_2180 - Curlin associated repeat., at 2,337,019 to 2,338,083
_2180
Position (kb)
2334
2335
2336
2337 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2333.353 kb on + strand, within Psest_2176 at 2333.353 kb on + strand, within Psest_2176 at 2333.354 kb on - strand, within Psest_2176 at 2333.354 kb on - strand, within Psest_2176 at 2333.354 kb on - strand, within Psest_2176 at 2333.354 kb on - strand, within Psest_2176 at 2333.354 kb on - strand, within Psest_2176 at 2333.354 kb on - strand, within Psest_2176 at 2333.537 kb on - strand, within Psest_2177 at 2333.644 kb on + strand, within Psest_2177 at 2333.644 kb on + strand, within Psest_2177 at 2333.645 kb on - strand, within Psest_2177 at 2333.645 kb on - strand, within Psest_2177 at 2333.645 kb on - strand, within Psest_2177 at 2333.645 kb on - strand, within Psest_2177 at 2333.645 kb on - strand, within Psest_2177 at 2333.645 kb on - strand, within Psest_2177 at 2333.687 kb on + strand at 2333.693 kb on + strand at 2333.693 kb on + strand at 2333.693 kb on + strand at 2333.694 kb on - strand at 2333.694 kb on - strand at 2333.709 kb on - strand at 2333.776 kb on - strand at 2333.795 kb on + strand at 2333.795 kb on + strand at 2333.795 kb on + strand at 2333.796 kb on - strand at 2333.807 kb on + strand at 2333.808 kb on - strand at 2333.808 kb on - strand at 2333.843 kb on + strand at 2333.844 kb on - strand at 2333.916 kb on - strand at 2333.937 kb on + strand at 2333.937 kb on + strand at 2333.937 kb on + strand at 2333.937 kb on + strand at 2333.937 kb on + strand at 2333.937 kb on + strand at 2333.937 kb on + strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.938 kb on - strand at 2333.941 kb on + strand at 2333.941 kb on + strand at 2333.942 kb on - strand at 2333.942 kb on - strand at 2333.942 kb on - strand at 2333.944 kb on + strand at 2333.944 kb on + strand at 2333.944 kb on + strand at 2333.944 kb on + strand at 2333.945 kb on - strand at 2334.259 kb on - strand at 2334.307 kb on - strand at 2334.426 kb on + strand at 2334.426 kb on + strand at 2334.523 kb on + strand at 2334.523 kb on + strand at 2334.523 kb on + strand at 2334.523 kb on + strand at 2334.524 kb on - strand at 2334.524 kb on - strand at 2334.544 kb on - strand at 2334.544 kb on - strand at 2334.837 kb on - strand, within Psest_2179 at 2334.929 kb on + strand, within Psest_2179 at 2334.929 kb on + strand, within Psest_2179 at 2334.930 kb on - strand, within Psest_2179 at 2335.102 kb on + strand, within Psest_2179 at 2335.103 kb on - strand, within Psest_2179 at 2335.103 kb on - strand, within Psest_2179 at 2335.103 kb on - strand, within Psest_2179 at 2335.284 kb on + strand, within Psest_2179 at 2335.287 kb on + strand, within Psest_2179 at 2335.287 kb on + strand, within Psest_2179 at 2335.287 kb on + strand, within Psest_2179 at 2335.287 kb on + strand, within Psest_2179 at 2335.287 kb on + strand, within Psest_2179 at 2335.287 kb on + strand, within Psest_2179 at 2335.288 kb on - strand, within Psest_2179 at 2335.288 kb on - strand, within Psest_2179 at 2335.288 kb on - strand, within Psest_2179 at 2335.497 kb on + strand, within Psest_2179 at 2335.497 kb on + strand, within Psest_2179 at 2335.497 kb on + strand, within Psest_2179 at 2335.497 kb on + strand, within Psest_2179 at 2335.498 kb on - strand, within Psest_2179 at 2335.498 kb on - strand, within Psest_2179 at 2335.498 kb on - strand, within Psest_2179 at 2335.498 kb on - strand, within Psest_2179 at 2335.498 kb on - strand, within Psest_2179 at 2335.498 kb on - strand, within Psest_2179 at 2335.498 kb on - strand, within Psest_2179 at 2335.892 kb on + strand, within Psest_2179 at 2335.981 kb on + strand, within Psest_2179 at 2335.981 kb on + strand, within Psest_2179 at 2336.046 kb on + strand, within Psest_2179 at 2336.046 kb on + strand, within Psest_2179 at 2336.046 kb on + strand, within Psest_2179 at 2336.047 kb on - strand, within Psest_2179 at 2336.047 kb on - strand, within Psest_2179 at 2336.460 kb on - strand, within Psest_2179 at 2336.502 kb on - strand, within Psest_2179 at 2336.502 kb on - strand, within Psest_2179 at 2336.529 kb on + strand, within Psest_2179 at 2336.999 kb on + strand at 2336.999 kb on + strand at 2336.999 kb on + strand at 2336.999 kb on + strand at 2337.000 kb on - strand at 2337.108 kb on + strand at 2337.109 kb on - strand at 2337.111 kb on + strand at 2337.331 kb on + strand, within Psest_2180 at 2337.332 kb on - strand, within Psest_2180 at 2337.332 kb on - strand, within Psest_2180 at 2337.332 kb on - strand, within Psest_2180 at 2337.332 kb on - strand, within Psest_2180 at 2337.332 kb on - strand, within Psest_2180 at 2337.442 kb on - strand, within Psest_2180 at 2337.453 kb on - strand, within Psest_2180 at 2337.464 kb on - strand, within Psest_2180 at 2337.464 kb on - strand, within Psest_2180 at 2337.464 kb on - strand, within Psest_2180 at 2337.467 kb on - strand, within Psest_2180 at 2337.483 kb on + strand, within Psest_2180 at 2337.762 kb on + strand, within Psest_2180
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 2,333,353 + Psest_2176 0.81 +1.1 2,333,353 + Psest_2176 0.81 -0.2 2,333,354 - Psest_2176 0.81 -2.0 2,333,354 - Psest_2176 0.81 +1.0 2,333,354 - Psest_2176 0.81 -1.4 2,333,354 - Psest_2176 0.81 -1.6 2,333,354 - Psest_2176 0.81 -0.9 2,333,354 - Psest_2176 0.81 -1.0 2,333,537 - Psest_2177 0.43 +2.7 2,333,644 + Psest_2177 0.82 +1.7 2,333,644 + Psest_2177 0.82 -2.0 2,333,645 - Psest_2177 0.82 -1.4 2,333,645 - Psest_2177 0.82 -2.0 2,333,645 - Psest_2177 0.82 +1.5 2,333,645 - Psest_2177 0.82 +0.2 2,333,645 - Psest_2177 0.82 -2.2 2,333,645 - Psest_2177 0.82 -1.2 2,333,687 + +0.7 2,333,693 + -1.3 2,333,693 + -0.3 2,333,693 + -0.1 2,333,694 - +0.6 2,333,694 - +0.1 2,333,709 - +1.9 2,333,776 - -2.0 2,333,795 + +0.8 2,333,795 + +0.8 2,333,795 + -0.2 2,333,796 - -1.7 2,333,807 + +0.7 2,333,808 - -0.4 2,333,808 - -1.1 2,333,843 + +0.4 2,333,844 - -0.8 2,333,916 - +0.6 2,333,937 + +0.9 2,333,937 + -1.3 2,333,937 + -0.3 2,333,937 + -0.6 2,333,937 + +0.8 2,333,937 + -0.3 2,333,937 + -1.2 2,333,938 - -0.5 2,333,938 - +0.3 2,333,938 - -0.8 2,333,938 - -1.2 2,333,938 - +1.1 2,333,938 - -0.2 2,333,938 - -0.1 2,333,938 - -0.9 2,333,938 - +2.1 2,333,938 - +0.9 2,333,941 + -0.7 2,333,941 + -2.9 2,333,942 - -2.2 2,333,942 - -1.4 2,333,942 - -2.6 2,333,944 + +1.2 2,333,944 + -0.1 2,333,944 + +0.6 2,333,944 + +0.8 2,333,945 - -1.5 2,334,259 - -2.6 2,334,307 - -1.5 2,334,426 + -0.7 2,334,426 + -1.2 2,334,523 + -0.4 2,334,523 + -0.9 2,334,523 + +0.1 2,334,523 + +0.8 2,334,524 - +0.3 2,334,524 - -1.6 2,334,544 - +1.8 2,334,544 - +0.0 2,334,837 - Psest_2179 0.21 +2.4 2,334,929 + Psest_2179 0.25 -1.3 2,334,929 + Psest_2179 0.25 +0.1 2,334,930 - Psest_2179 0.25 +2.2 2,335,102 + Psest_2179 0.32 +0.6 2,335,103 - Psest_2179 0.32 +1.0 2,335,103 - Psest_2179 0.32 +1.7 2,335,103 - Psest_2179 0.32 -2.0 2,335,284 + Psest_2179 0.39 +0.2 2,335,287 + Psest_2179 0.39 +1.3 2,335,287 + Psest_2179 0.39 +1.0 2,335,287 + Psest_2179 0.39 -2.3 2,335,287 + Psest_2179 0.39 -1.6 2,335,287 + Psest_2179 0.39 +2.4 2,335,287 + Psest_2179 0.39 +3.8 2,335,288 - Psest_2179 0.39 -1.7 2,335,288 - Psest_2179 0.39 -0.9 2,335,288 - Psest_2179 0.39 -0.4 2,335,497 + Psest_2179 0.47 +2.8 2,335,497 + Psest_2179 0.47 +0.3 2,335,497 + Psest_2179 0.47 -0.4 2,335,497 + Psest_2179 0.47 -1.6 2,335,498 - Psest_2179 0.47 +2.3 2,335,498 - Psest_2179 0.47 -1.3 2,335,498 - Psest_2179 0.47 -2.1 2,335,498 - Psest_2179 0.47 +4.1 2,335,498 - Psest_2179 0.47 -0.8 2,335,498 - Psest_2179 0.47 -1.8 2,335,498 - Psest_2179 0.47 +2.3 2,335,892 + Psest_2179 0.62 +0.5 2,335,981 + Psest_2179 0.66 -0.9 2,335,981 + Psest_2179 0.66 +1.7 2,336,046 + Psest_2179 0.68 -0.3 2,336,046 + Psest_2179 0.68 -0.9 2,336,046 + Psest_2179 0.68 -2.0 2,336,047 - Psest_2179 0.68 -1.0 2,336,047 - Psest_2179 0.68 -0.9 2,336,460 - Psest_2179 0.85 -1.4 2,336,502 - Psest_2179 0.86 +1.4 2,336,502 - Psest_2179 0.86 +0.4 2,336,529 + Psest_2179 0.87 -0.9 2,336,999 + -2.6 2,336,999 + +0.5 2,336,999 + -0.8 2,336,999 + -0.2 2,337,000 - +0.1 2,337,108 + +1.6 2,337,109 - -0.4 2,337,111 + -0.3 2,337,331 + Psest_2180 0.29 -0.3 2,337,332 - Psest_2180 0.29 -1.6 2,337,332 - Psest_2180 0.29 -3.3 2,337,332 - Psest_2180 0.29 -0.4 2,337,332 - Psest_2180 0.29 -1.6 2,337,332 - Psest_2180 0.29 +0.9 2,337,442 - Psest_2180 0.40 -2.4 2,337,453 - Psest_2180 0.41 +1.4 2,337,464 - Psest_2180 0.42 +0.7 2,337,464 - Psest_2180 0.42 +1.5 2,337,464 - Psest_2180 0.42 +0.1 2,337,467 - Psest_2180 0.42 +1.2 2,337,483 + Psest_2180 0.44 +1.6 2,337,762 + Psest_2180 0.70 +1.9
Or see this region's nucleotide sequence