Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1899 and Psest_1900 are separated by 108 nucleotides Psest_1900 and Psest_1901 are separated by 10 nucleotides Psest_1901 and Psest_1902 are separated by 80 nucleotides
Psest_1899: Psest_1899 - ABC-type sugar transport systems, ATPase components, at 2,009,054 to 2,010,226
_1899
Psest_1900: Psest_1900 - Maltoporin (phage lambda and maltose receptor), at 2,010,335 to 2,011,558
_1900
Psest_1901: Psest_1901 - hypothetical protein, at 2,011,569 to 2,012,348
_1901
Psest_1902: Psest_1902 - hypothetical protein, at 2,012,429 to 2,013,388
_1902
Position (kb)
2010
2011
2012 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2009.522 kb on + strand, within Psest_1899 at 2009.522 kb on + strand, within Psest_1899 at 2009.522 kb on + strand, within Psest_1899 at 2009.624 kb on + strand, within Psest_1899 at 2009.624 kb on + strand, within Psest_1899 at 2009.624 kb on + strand, within Psest_1899 at 2009.624 kb on + strand, within Psest_1899 at 2009.625 kb on - strand, within Psest_1899 at 2009.625 kb on - strand, within Psest_1899 at 2009.625 kb on - strand, within Psest_1899 at 2009.625 kb on - strand, within Psest_1899 at 2009.625 kb on - strand, within Psest_1899 at 2009.723 kb on + strand, within Psest_1899 at 2009.723 kb on + strand, within Psest_1899 at 2009.724 kb on - strand, within Psest_1899 at 2009.724 kb on - strand, within Psest_1899 at 2009.724 kb on - strand, within Psest_1899 at 2010.314 kb on - strand at 2010.425 kb on + strand at 2010.426 kb on - strand at 2010.504 kb on - strand, within Psest_1900 at 2010.504 kb on - strand, within Psest_1900 at 2010.560 kb on + strand, within Psest_1900 at 2010.560 kb on + strand, within Psest_1900 at 2010.561 kb on - strand, within Psest_1900 at 2010.561 kb on - strand, within Psest_1900 at 2010.612 kb on - strand, within Psest_1900 at 2010.612 kb on - strand, within Psest_1900 at 2010.620 kb on + strand, within Psest_1900 at 2010.620 kb on + strand, within Psest_1900 at 2010.621 kb on - strand, within Psest_1900 at 2010.678 kb on - strand, within Psest_1900 at 2010.740 kb on + strand, within Psest_1900 at 2010.741 kb on - strand, within Psest_1900 at 2010.741 kb on - strand, within Psest_1900 at 2010.741 kb on - strand, within Psest_1900 at 2010.741 kb on - strand, within Psest_1900 at 2010.741 kb on - strand, within Psest_1900 at 2010.741 kb on - strand, within Psest_1900 at 2010.760 kb on + strand, within Psest_1900 at 2010.760 kb on + strand, within Psest_1900 at 2010.760 kb on + strand, within Psest_1900 at 2010.760 kb on + strand, within Psest_1900 at 2010.760 kb on + strand, within Psest_1900 at 2010.761 kb on - strand, within Psest_1900 at 2010.773 kb on + strand, within Psest_1900 at 2010.773 kb on + strand, within Psest_1900 at 2010.773 kb on + strand, within Psest_1900 at 2010.774 kb on - strand, within Psest_1900 at 2010.774 kb on - strand, within Psest_1900 at 2010.774 kb on - strand, within Psest_1900 at 2010.776 kb on + strand, within Psest_1900 at 2010.776 kb on + strand, within Psest_1900 at 2010.777 kb on - strand, within Psest_1900 at 2010.836 kb on + strand, within Psest_1900 at 2010.836 kb on + strand, within Psest_1900 at 2010.836 kb on + strand, within Psest_1900 at 2010.837 kb on - strand, within Psest_1900 at 2010.843 kb on - strand, within Psest_1900 at 2010.843 kb on - strand, within Psest_1900 at 2010.956 kb on + strand, within Psest_1900 at 2010.956 kb on + strand, within Psest_1900 at 2010.956 kb on + strand, within Psest_1900 at 2010.956 kb on + strand, within Psest_1900 at 2010.957 kb on - strand, within Psest_1900 at 2011.091 kb on + strand, within Psest_1900 at 2011.093 kb on + strand, within Psest_1900 at 2011.093 kb on + strand, within Psest_1900 at 2011.094 kb on - strand, within Psest_1900 at 2011.145 kb on + strand, within Psest_1900 at 2011.146 kb on - strand, within Psest_1900 at 2011.146 kb on - strand, within Psest_1900 at 2011.262 kb on + strand, within Psest_1900 at 2011.262 kb on + strand, within Psest_1900 at 2011.262 kb on + strand, within Psest_1900 at 2011.262 kb on + strand, within Psest_1900 at 2011.263 kb on - strand, within Psest_1900 at 2011.415 kb on + strand, within Psest_1900 at 2011.415 kb on + strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.416 kb on - strand, within Psest_1900 at 2011.418 kb on + strand, within Psest_1900 at 2011.418 kb on + strand, within Psest_1900 at 2011.419 kb on - strand, within Psest_1900 at 2011.419 kb on - strand, within Psest_1900 at 2011.424 kb on + strand, within Psest_1900 at 2011.425 kb on - strand, within Psest_1900 at 2011.536 kb on + strand at 2011.537 kb on - strand at 2011.542 kb on + strand at 2011.542 kb on + strand at 2011.543 kb on - strand at 2011.543 kb on - strand at 2011.543 kb on - strand at 2011.543 kb on - strand at 2011.556 kb on + strand at 2011.556 kb on + strand at 2011.955 kb on - strand, within Psest_1901 at 2012.097 kb on + strand, within Psest_1901 at 2012.097 kb on + strand, within Psest_1901 at 2012.097 kb on + strand, within Psest_1901 at 2012.098 kb on - strand, within Psest_1901 at 2012.098 kb on - strand, within Psest_1901 at 2012.098 kb on - strand, within Psest_1901 at 2012.098 kb on - strand, within Psest_1901 at 2012.520 kb on + strand at 2012.520 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 2,009,522 + Psest_1899 0.40 +0.2 2,009,522 + Psest_1899 0.40 +0.3 2,009,522 + Psest_1899 0.40 +1.6 2,009,624 + Psest_1899 0.49 +1.2 2,009,624 + Psest_1899 0.49 -2.5 2,009,624 + Psest_1899 0.49 -1.3 2,009,624 + Psest_1899 0.49 +0.8 2,009,625 - Psest_1899 0.49 -0.2 2,009,625 - Psest_1899 0.49 +0.9 2,009,625 - Psest_1899 0.49 +2.4 2,009,625 - Psest_1899 0.49 +2.0 2,009,625 - Psest_1899 0.49 -0.7 2,009,723 + Psest_1899 0.57 -3.1 2,009,723 + Psest_1899 0.57 +0.8 2,009,724 - Psest_1899 0.57 -2.1 2,009,724 - Psest_1899 0.57 -1.1 2,009,724 - Psest_1899 0.57 +0.2 2,010,314 - -0.1 2,010,425 + +2.6 2,010,426 - -2.2 2,010,504 - Psest_1900 0.14 +0.9 2,010,504 - Psest_1900 0.14 +1.4 2,010,560 + Psest_1900 0.18 +1.4 2,010,560 + Psest_1900 0.18 +0.1 2,010,561 - Psest_1900 0.18 +2.1 2,010,561 - Psest_1900 0.18 -0.1 2,010,612 - Psest_1900 0.23 -0.2 2,010,612 - Psest_1900 0.23 +0.0 2,010,620 + Psest_1900 0.23 -0.1 2,010,620 + Psest_1900 0.23 +0.4 2,010,621 - Psest_1900 0.23 -0.2 2,010,678 - Psest_1900 0.28 +2.1 2,010,740 + Psest_1900 0.33 +0.8 2,010,741 - Psest_1900 0.33 -0.7 2,010,741 - Psest_1900 0.33 +0.5 2,010,741 - Psest_1900 0.33 -1.1 2,010,741 - Psest_1900 0.33 -0.4 2,010,741 - Psest_1900 0.33 -0.2 2,010,741 - Psest_1900 0.33 +2.2 2,010,760 + Psest_1900 0.35 +2.7 2,010,760 + Psest_1900 0.35 +0.5 2,010,760 + Psest_1900 0.35 -0.5 2,010,760 + Psest_1900 0.35 +0.2 2,010,760 + Psest_1900 0.35 +0.9 2,010,761 - Psest_1900 0.35 +1.0 2,010,773 + Psest_1900 0.36 -1.2 2,010,773 + Psest_1900 0.36 +2.4 2,010,773 + Psest_1900 0.36 +0.9 2,010,774 - Psest_1900 0.36 -0.0 2,010,774 - Psest_1900 0.36 -0.9 2,010,774 - Psest_1900 0.36 +1.1 2,010,776 + Psest_1900 0.36 -0.6 2,010,776 + Psest_1900 0.36 -1.0 2,010,777 - Psest_1900 0.36 +0.1 2,010,836 + Psest_1900 0.41 +2.1 2,010,836 + Psest_1900 0.41 -1.3 2,010,836 + Psest_1900 0.41 -0.1 2,010,837 - Psest_1900 0.41 -0.2 2,010,843 - Psest_1900 0.42 -0.7 2,010,843 - Psest_1900 0.42 +0.2 2,010,956 + Psest_1900 0.51 +0.2 2,010,956 + Psest_1900 0.51 +3.7 2,010,956 + Psest_1900 0.51 -0.5 2,010,956 + Psest_1900 0.51 +2.0 2,010,957 - Psest_1900 0.51 +1.0 2,011,091 + Psest_1900 0.62 +0.5 2,011,093 + Psest_1900 0.62 -1.1 2,011,093 + Psest_1900 0.62 +1.1 2,011,094 - Psest_1900 0.62 -0.5 2,011,145 + Psest_1900 0.66 +0.8 2,011,146 - Psest_1900 0.66 +0.1 2,011,146 - Psest_1900 0.66 +0.6 2,011,262 + Psest_1900 0.76 +2.0 2,011,262 + Psest_1900 0.76 -0.3 2,011,262 + Psest_1900 0.76 +0.1 2,011,262 + Psest_1900 0.76 +2.1 2,011,263 - Psest_1900 0.76 +1.0 2,011,415 + Psest_1900 0.88 +2.4 2,011,415 + Psest_1900 0.88 +0.1 2,011,416 - Psest_1900 0.88 +3.9 2,011,416 - Psest_1900 0.88 +1.3 2,011,416 - Psest_1900 0.88 -0.8 2,011,416 - Psest_1900 0.88 +0.8 2,011,416 - Psest_1900 0.88 +0.9 2,011,416 - Psest_1900 0.88 -2.0 2,011,416 - Psest_1900 0.88 -0.2 2,011,416 - Psest_1900 0.88 -1.1 2,011,418 + Psest_1900 0.88 +1.4 2,011,418 + Psest_1900 0.88 -0.6 2,011,419 - Psest_1900 0.89 +0.2 2,011,419 - Psest_1900 0.89 +0.2 2,011,424 + Psest_1900 0.89 +0.8 2,011,425 - Psest_1900 0.89 +1.0 2,011,536 + +0.1 2,011,537 - -0.9 2,011,542 + +1.5 2,011,542 + +2.6 2,011,543 - +0.7 2,011,543 - -0.1 2,011,543 - +2.4 2,011,543 - +0.8 2,011,556 + -0.7 2,011,556 + +2.7 2,011,955 - Psest_1901 0.49 -2.0 2,012,097 + Psest_1901 0.68 +1.0 2,012,097 + Psest_1901 0.68 +0.5 2,012,097 + Psest_1901 0.68 +0.8 2,012,098 - Psest_1901 0.68 -0.9 2,012,098 - Psest_1901 0.68 -1.8 2,012,098 - Psest_1901 0.68 -1.1 2,012,098 - Psest_1901 0.68 +3.1 2,012,520 + +1.8 2,012,520 + -0.7
Or see this region's nucleotide sequence