Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1855 and Psest_1856 are separated by 24 nucleotides Psest_1856 and Psest_1857 are separated by 83 nucleotides
Psest_1855: Psest_1855 - cysteine synthase A, at 1,959,445 to 1,960,419
_1855
Psest_1856: Psest_1856 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 1,960,444 to 1,961,403
_1856
Psest_1857: Psest_1857 - Isocitrate dehydrogenase kinase/phosphatase, at 1,961,487 to 1,963,208
_1857
Position (kb)
1960
1961
1962 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1959.455 kb on + strand at 1959.497 kb on + strand at 1959.707 kb on - strand, within Psest_1855 at 1959.758 kb on + strand, within Psest_1855 at 1959.862 kb on + strand, within Psest_1855 at 1959.862 kb on + strand, within Psest_1855 at 1959.862 kb on + strand, within Psest_1855 at 1959.862 kb on + strand, within Psest_1855 at 1959.863 kb on - strand, within Psest_1855 at 1959.863 kb on - strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.865 kb on + strand, within Psest_1855 at 1959.866 kb on - strand, within Psest_1855 at 1959.866 kb on - strand, within Psest_1855 at 1959.866 kb on - strand, within Psest_1855 at 1959.866 kb on - strand, within Psest_1855 at 1959.866 kb on - strand, within Psest_1855 at 1959.866 kb on - strand, within Psest_1855 at 1959.866 kb on - strand, within Psest_1855 at 1960.012 kb on + strand, within Psest_1855 at 1960.012 kb on + strand, within Psest_1855 at 1960.014 kb on + strand, within Psest_1855 at 1960.015 kb on - strand, within Psest_1855 at 1960.015 kb on - strand, within Psest_1855 at 1960.364 kb on - strand at 1960.417 kb on + strand at 1960.418 kb on - strand at 1960.418 kb on - strand at 1960.418 kb on - strand at 1960.538 kb on + strand at 1960.539 kb on - strand at 1960.539 kb on - strand at 1960.571 kb on - strand, within Psest_1856 at 1960.656 kb on - strand, within Psest_1856 at 1960.656 kb on - strand, within Psest_1856 at 1960.664 kb on + strand, within Psest_1856 at 1960.665 kb on - strand, within Psest_1856 at 1960.665 kb on - strand, within Psest_1856 at 1960.665 kb on - strand, within Psest_1856 at 1961.115 kb on - strand, within Psest_1856 at 1961.449 kb on - strand at 1961.649 kb on + strand at 1961.748 kb on + strand, within Psest_1857 at 1961.748 kb on + strand, within Psest_1857 at 1961.748 kb on + strand, within Psest_1857 at 1961.917 kb on - strand, within Psest_1857 at 1961.917 kb on - strand, within Psest_1857 at 1961.917 kb on - strand, within Psest_1857 at 1962.118 kb on - strand, within Psest_1857 at 1962.261 kb on + strand, within Psest_1857 at 1962.261 kb on + strand, within Psest_1857 at 1962.261 kb on + strand, within Psest_1857 at 1962.261 kb on + strand, within Psest_1857 at 1962.262 kb on - strand, within Psest_1857 at 1962.262 kb on - strand, within Psest_1857 at 1962.262 kb on - strand, within Psest_1857 at 1962.262 kb on - strand, within Psest_1857 at 1962.262 kb on - strand, within Psest_1857 at 1962.345 kb on + strand, within Psest_1857 at 1962.345 kb on + strand, within Psest_1857 at 1962.345 kb on + strand, within Psest_1857 at 1962.345 kb on + strand, within Psest_1857 at 1962.346 kb on - strand, within Psest_1857 at 1962.346 kb on - strand, within Psest_1857 at 1962.347 kb on + strand, within Psest_1857 at 1962.348 kb on - strand, within Psest_1857 at 1962.348 kb on - strand, within Psest_1857 at 1962.348 kb on - strand, within Psest_1857 at 1962.348 kb on - strand, within Psest_1857 at 1962.354 kb on - strand, within Psest_1857 at 1962.355 kb on + strand, within Psest_1857 at 1962.355 kb on + strand, within Psest_1857 at 1962.355 kb on + strand, within Psest_1857 at 1962.356 kb on - strand, within Psest_1857 at 1962.356 kb on - strand, within Psest_1857 at 1962.363 kb on + strand, within Psest_1857 at 1962.363 kb on + strand, within Psest_1857 at 1962.363 kb on + strand, within Psest_1857 at 1962.364 kb on - strand, within Psest_1857 at 1962.364 kb on - strand, within Psest_1857 at 1962.364 kb on - strand, within Psest_1857 at 1962.387 kb on + strand, within Psest_1857 at 1962.388 kb on - strand, within Psest_1857
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 1,959,455 + +1.1 1,959,497 + -0.4 1,959,707 - Psest_1855 0.27 +0.2 1,959,758 + Psest_1855 0.32 -0.9 1,959,862 + Psest_1855 0.43 -0.7 1,959,862 + Psest_1855 0.43 -3.3 1,959,862 + Psest_1855 0.43 -1.7 1,959,862 + Psest_1855 0.43 -1.0 1,959,863 - Psest_1855 0.43 -2.4 1,959,863 - Psest_1855 0.43 -0.7 1,959,865 + Psest_1855 0.43 +0.7 1,959,865 + Psest_1855 0.43 -2.6 1,959,865 + Psest_1855 0.43 +1.7 1,959,865 + Psest_1855 0.43 +1.4 1,959,865 + Psest_1855 0.43 -2.1 1,959,865 + Psest_1855 0.43 -0.4 1,959,865 + Psest_1855 0.43 -1.3 1,959,865 + Psest_1855 0.43 -3.1 1,959,865 + Psest_1855 0.43 +0.2 1,959,866 - Psest_1855 0.43 +1.3 1,959,866 - Psest_1855 0.43 +0.1 1,959,866 - Psest_1855 0.43 +0.7 1,959,866 - Psest_1855 0.43 -0.2 1,959,866 - Psest_1855 0.43 -2.3 1,959,866 - Psest_1855 0.43 +1.6 1,959,866 - Psest_1855 0.43 +1.2 1,960,012 + Psest_1855 0.58 -1.4 1,960,012 + Psest_1855 0.58 +0.6 1,960,014 + Psest_1855 0.58 -1.3 1,960,015 - Psest_1855 0.58 +0.1 1,960,015 - Psest_1855 0.58 +0.6 1,960,364 - -0.7 1,960,417 + +0.7 1,960,418 - +0.7 1,960,418 - -2.2 1,960,418 - -0.8 1,960,538 + -1.7 1,960,539 - +1.5 1,960,539 - -3.4 1,960,571 - Psest_1856 0.13 +0.4 1,960,656 - Psest_1856 0.22 -0.1 1,960,656 - Psest_1856 0.22 -0.1 1,960,664 + Psest_1856 0.23 -0.7 1,960,665 - Psest_1856 0.23 -1.7 1,960,665 - Psest_1856 0.23 -1.3 1,960,665 - Psest_1856 0.23 -2.0 1,961,115 - Psest_1856 0.70 +0.1 1,961,449 - -1.2 1,961,649 + +2.4 1,961,748 + Psest_1857 0.15 +1.1 1,961,748 + Psest_1857 0.15 -2.8 1,961,748 + Psest_1857 0.15 -2.9 1,961,917 - Psest_1857 0.25 -1.0 1,961,917 - Psest_1857 0.25 +0.6 1,961,917 - Psest_1857 0.25 -0.2 1,962,118 - Psest_1857 0.37 +1.3 1,962,261 + Psest_1857 0.45 +0.2 1,962,261 + Psest_1857 0.45 -0.2 1,962,261 + Psest_1857 0.45 +0.5 1,962,261 + Psest_1857 0.45 -1.2 1,962,262 - Psest_1857 0.45 +0.8 1,962,262 - Psest_1857 0.45 +0.7 1,962,262 - Psest_1857 0.45 -1.2 1,962,262 - Psest_1857 0.45 -1.8 1,962,262 - Psest_1857 0.45 +1.2 1,962,345 + Psest_1857 0.50 +0.5 1,962,345 + Psest_1857 0.50 -0.6 1,962,345 + Psest_1857 0.50 -2.4 1,962,345 + Psest_1857 0.50 -0.3 1,962,346 - Psest_1857 0.50 -1.1 1,962,346 - Psest_1857 0.50 -0.2 1,962,347 + Psest_1857 0.50 +0.1 1,962,348 - Psest_1857 0.50 -2.1 1,962,348 - Psest_1857 0.50 -2.9 1,962,348 - Psest_1857 0.50 +1.4 1,962,348 - Psest_1857 0.50 -1.3 1,962,354 - Psest_1857 0.50 +0.6 1,962,355 + Psest_1857 0.50 +1.1 1,962,355 + Psest_1857 0.50 -0.6 1,962,355 + Psest_1857 0.50 +0.1 1,962,356 - Psest_1857 0.50 +1.4 1,962,356 - Psest_1857 0.50 +2.4 1,962,363 + Psest_1857 0.51 +2.9 1,962,363 + Psest_1857 0.51 -2.3 1,962,363 + Psest_1857 0.51 +3.0 1,962,364 - Psest_1857 0.51 -1.2 1,962,364 - Psest_1857 0.51 -1.9 1,962,364 - Psest_1857 0.51 +0.5 1,962,387 + Psest_1857 0.52 -1.1 1,962,388 - Psest_1857 0.52 -1.7
Or see this region's nucleotide sequence