Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1644 and Psest_1645 are separated by 194 nucleotides Psest_1645 and Psest_1646 overlap by 23 nucleotides Psest_1646 and Psest_1647 are separated by 177 nucleotides
Psest_1644: Psest_1644 - Erythromycin esterase homolog, at 1,747,561 to 1,748,895
_1644
Psest_1645: Psest_1645 - hypothetical protein, at 1,749,090 to 1,749,323
_1645
Psest_1646: Psest_1646 - Predicted ICC-like phosphoesterases, at 1,749,301 to 1,749,990
_1646
Psest_1647: Psest_1647 - Lhr-like helicases, at 1,750,168 to 1,752,759
_1647
Position (kb)
1749
1750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1748.325 kb on - strand, within Psest_1644 at 1748.363 kb on + strand, within Psest_1644 at 1748.420 kb on - strand, within Psest_1644 at 1748.715 kb on - strand, within Psest_1644 at 1748.715 kb on - strand, within Psest_1644 at 1748.895 kb on + strand at 1748.896 kb on - strand at 1748.896 kb on - strand at 1748.968 kb on + strand at 1748.968 kb on + strand at 1748.969 kb on - strand at 1748.969 kb on - strand at 1748.969 kb on - strand at 1748.969 kb on - strand at 1748.977 kb on + strand at 1748.977 kb on + strand at 1749.049 kb on + strand at 1749.050 kb on - strand at 1749.052 kb on + strand at 1749.053 kb on - strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.111 kb on + strand at 1749.112 kb on - strand at 1749.112 kb on - strand at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.371 kb on + strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.372 kb on - strand, within Psest_1646 at 1749.621 kb on + strand, within Psest_1646 at 1749.622 kb on - strand, within Psest_1646 at 1749.853 kb on + strand, within Psest_1646 at 1749.853 kb on + strand, within Psest_1646 at 1749.970 kb on + strand at 1750.542 kb on - strand, within Psest_1647 at 1750.638 kb on + strand, within Psest_1647 at 1750.638 kb on + strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.639 kb on - strand, within Psest_1647 at 1750.976 kb on - strand, within Psest_1647
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 1,748,325 - Psest_1644 0.57 +1.7 1,748,363 + Psest_1644 0.60 -1.2 1,748,420 - Psest_1644 0.64 +0.7 1,748,715 - Psest_1644 0.86 +0.1 1,748,715 - Psest_1644 0.86 +0.3 1,748,895 + -0.8 1,748,896 - -1.8 1,748,896 - -0.3 1,748,968 + +1.6 1,748,968 + -1.9 1,748,969 - -1.6 1,748,969 - +1.3 1,748,969 - +6.0 1,748,969 - +0.7 1,748,977 + -1.8 1,748,977 + -0.6 1,749,049 + +1.1 1,749,050 - +0.6 1,749,052 + -2.5 1,749,053 - -0.7 1,749,111 + +0.7 1,749,111 + +0.5 1,749,111 + +2.6 1,749,111 + +1.3 1,749,111 + -2.0 1,749,112 - +2.2 1,749,112 - -0.1 1,749,371 + Psest_1646 0.10 +1.2 1,749,371 + Psest_1646 0.10 -0.1 1,749,371 + Psest_1646 0.10 -0.6 1,749,371 + Psest_1646 0.10 -1.7 1,749,371 + Psest_1646 0.10 -0.9 1,749,371 + Psest_1646 0.10 +0.3 1,749,371 + Psest_1646 0.10 -0.1 1,749,371 + Psest_1646 0.10 +0.1 1,749,372 - Psest_1646 0.10 +0.4 1,749,372 - Psest_1646 0.10 -0.2 1,749,372 - Psest_1646 0.10 -0.6 1,749,372 - Psest_1646 0.10 -1.7 1,749,372 - Psest_1646 0.10 -3.6 1,749,621 + Psest_1646 0.46 +0.5 1,749,622 - Psest_1646 0.47 -1.5 1,749,853 + Psest_1646 0.80 +1.9 1,749,853 + Psest_1646 0.80 -1.9 1,749,970 + -0.6 1,750,542 - Psest_1647 0.14 +0.0 1,750,638 + Psest_1647 0.18 +2.1 1,750,638 + Psest_1647 0.18 +2.7 1,750,639 - Psest_1647 0.18 +2.3 1,750,639 - Psest_1647 0.18 +1.1 1,750,639 - Psest_1647 0.18 -2.6 1,750,639 - Psest_1647 0.18 -1.2 1,750,976 - Psest_1647 0.31 +4.4
Or see this region's nucleotide sequence