Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1513 and Psest_1514 are separated by 21 nucleotides Psest_1514 and Psest_1515 are separated by 227 nucleotides Psest_1515 and Psest_1516 are separated by 16 nucleotides
Psest_1513: Psest_1513 - Peroxiredoxin, at 1,612,761 to 1,613,234
_1513
Psest_1514: Psest_1514 - Glycine cleavage system regulatory protein, at 1,613,256 to 1,613,816
_1514
Psest_1515: Psest_1515 - dihydrodipicolinate synthase, at 1,614,044 to 1,614,922
_1515
Psest_1516: Psest_1516 - Uncharacterized lipoprotein, at 1,614,939 to 1,616,036
_1516
Position (kb)
1614
1615 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1613.100 kb on - strand, within Psest_1513 at 1613.131 kb on + strand, within Psest_1513 at 1613.131 kb on + strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.132 kb on - strand, within Psest_1513 at 1613.417 kb on - strand, within Psest_1514 at 1613.512 kb on + strand, within Psest_1514 at 1613.512 kb on + strand, within Psest_1514 at 1613.512 kb on + strand, within Psest_1514 at 1613.512 kb on + strand, within Psest_1514 at 1613.512 kb on + strand, within Psest_1514 at 1613.513 kb on - strand, within Psest_1514 at 1613.513 kb on - strand, within Psest_1514 at 1613.513 kb on - strand, within Psest_1514 at 1613.513 kb on - strand, within Psest_1514 at 1613.513 kb on - strand, within Psest_1514 at 1613.521 kb on + strand, within Psest_1514 at 1613.522 kb on - strand, within Psest_1514 at 1614.002 kb on + strand at 1614.002 kb on + strand at 1614.002 kb on + strand at 1615.260 kb on + strand, within Psest_1516 at 1615.479 kb on + strand, within Psest_1516 at 1615.479 kb on + strand, within Psest_1516 at 1615.479 kb on + strand, within Psest_1516 at 1615.479 kb on + strand, within Psest_1516 at 1615.800 kb on + strand, within Psest_1516 at 1615.804 kb on - strand, within Psest_1516
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 1,613,100 - Psest_1513 0.72 -0.9 1,613,131 + Psest_1513 0.78 +0.4 1,613,131 + Psest_1513 0.78 -1.8 1,613,132 - Psest_1513 0.78 -2.2 1,613,132 - Psest_1513 0.78 -1.3 1,613,132 - Psest_1513 0.78 +3.3 1,613,132 - Psest_1513 0.78 -2.0 1,613,132 - Psest_1513 0.78 +0.9 1,613,132 - Psest_1513 0.78 -2.1 1,613,132 - Psest_1513 0.78 +0.6 1,613,132 - Psest_1513 0.78 +1.2 1,613,132 - Psest_1513 0.78 -0.9 1,613,132 - Psest_1513 0.78 +0.2 1,613,132 - Psest_1513 0.78 -1.2 1,613,132 - Psest_1513 0.78 -1.9 1,613,417 - Psest_1514 0.29 -1.4 1,613,512 + Psest_1514 0.46 -1.7 1,613,512 + Psest_1514 0.46 -1.3 1,613,512 + Psest_1514 0.46 -0.2 1,613,512 + Psest_1514 0.46 -0.9 1,613,512 + Psest_1514 0.46 -2.1 1,613,513 - Psest_1514 0.46 -1.7 1,613,513 - Psest_1514 0.46 -0.8 1,613,513 - Psest_1514 0.46 -1.5 1,613,513 - Psest_1514 0.46 -2.9 1,613,513 - Psest_1514 0.46 -2.0 1,613,521 + Psest_1514 0.47 +0.3 1,613,522 - Psest_1514 0.47 -3.3 1,614,002 + +0.5 1,614,002 + -2.1 1,614,002 + -1.2 1,615,260 + Psest_1516 0.29 -0.3 1,615,479 + Psest_1516 0.49 +0.5 1,615,479 + Psest_1516 0.49 -0.6 1,615,479 + Psest_1516 0.49 -0.5 1,615,479 + Psest_1516 0.49 +1.5 1,615,800 + Psest_1516 0.78 +4.1 1,615,804 - Psest_1516 0.79 +1.5
Or see this region's nucleotide sequence