Experiment: RCH2 grown on a colony of N2C3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1277 and Psest_1278 are separated by 71 nucleotides Psest_1278 and Psest_1279 are separated by 19 nucleotides Psest_1279 and Psest_1280 are separated by 81 nucleotides
Psest_1277: Psest_1277 - 2-isopropylmalate synthase, yeast type, at 1,380,523 to 1,382,193
_1277
Psest_1278: Psest_1278 - hypothetical protein, at 1,382,265 to 1,382,438
_1278
Psest_1279: Psest_1279 - hypothetical protein, at 1,382,458 to 1,382,841
_1279
Psest_1280: Psest_1280 - TonB-dependent copper receptor, at 1,382,923 to 1,385,130
_1280
Position (kb)
1382
1383 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1381.471 kb on + strand, within Psest_1277 at 1381.472 kb on - strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.523 kb on + strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.524 kb on - strand, within Psest_1277 at 1381.528 kb on - strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.805 kb on + strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1381.806 kb on - strand, within Psest_1277 at 1382.459 kb on + strand at 1382.460 kb on - strand at 1382.517 kb on + strand, within Psest_1279 at 1382.518 kb on - strand, within Psest_1279 at 1382.518 kb on - strand, within Psest_1279 at 1382.518 kb on - strand, within Psest_1279 at 1382.566 kb on + strand, within Psest_1279 at 1382.566 kb on + strand, within Psest_1279 at 1382.566 kb on + strand, within Psest_1279 at 1382.567 kb on - strand, within Psest_1279 at 1382.567 kb on - strand, within Psest_1279 at 1382.923 kb on - strand at 1382.935 kb on - strand at 1382.935 kb on - strand at 1383.117 kb on + strand at 1383.118 kb on - strand at 1383.209 kb on - strand, within Psest_1280 at 1383.240 kb on - strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.364 kb on + strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.365 kb on - strand, within Psest_1280 at 1383.616 kb on + strand, within Psest_1280 at 1383.616 kb on + strand, within Psest_1280 at 1383.617 kb on - strand, within Psest_1280 at 1383.666 kb on + strand, within Psest_1280 at 1383.739 kb on + strand, within Psest_1280 at 1383.748 kb on + strand, within Psest_1280 at 1383.749 kb on - strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.835 kb on + strand, within Psest_1280 at 1383.836 kb on - strand, within Psest_1280
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2C3 remove 1,381,471 + Psest_1277 0.57 +1.6 1,381,472 - Psest_1277 0.57 -2.1 1,381,523 + Psest_1277 0.60 -0.3 1,381,523 + Psest_1277 0.60 +1.2 1,381,523 + Psest_1277 0.60 -0.7 1,381,523 + Psest_1277 0.60 -1.6 1,381,523 + Psest_1277 0.60 -1.3 1,381,524 - Psest_1277 0.60 -1.6 1,381,524 - Psest_1277 0.60 -0.0 1,381,524 - Psest_1277 0.60 -0.4 1,381,524 - Psest_1277 0.60 +3.4 1,381,528 - Psest_1277 0.60 -0.0 1,381,805 + Psest_1277 0.77 +0.4 1,381,805 + Psest_1277 0.77 +1.6 1,381,805 + Psest_1277 0.77 -0.0 1,381,805 + Psest_1277 0.77 +0.0 1,381,805 + Psest_1277 0.77 +1.7 1,381,805 + Psest_1277 0.77 +0.9 1,381,805 + Psest_1277 0.77 +0.9 1,381,805 + Psest_1277 0.77 -2.2 1,381,805 + Psest_1277 0.77 +1.9 1,381,805 + Psest_1277 0.77 -0.2 1,381,806 - Psest_1277 0.77 -0.5 1,381,806 - Psest_1277 0.77 -1.1 1,381,806 - Psest_1277 0.77 -0.4 1,381,806 - Psest_1277 0.77 -0.2 1,381,806 - Psest_1277 0.77 -0.7 1,381,806 - Psest_1277 0.77 +1.1 1,381,806 - Psest_1277 0.77 -2.4 1,381,806 - Psest_1277 0.77 +0.7 1,382,459 + -0.7 1,382,460 - -1.1 1,382,517 + Psest_1279 0.15 -2.5 1,382,518 - Psest_1279 0.16 +0.6 1,382,518 - Psest_1279 0.16 +1.6 1,382,518 - Psest_1279 0.16 +1.0 1,382,566 + Psest_1279 0.28 -1.9 1,382,566 + Psest_1279 0.28 -1.7 1,382,566 + Psest_1279 0.28 -1.2 1,382,567 - Psest_1279 0.28 -0.9 1,382,567 - Psest_1279 0.28 +1.0 1,382,923 - -0.4 1,382,935 - +2.0 1,382,935 - +0.6 1,383,117 + +1.6 1,383,118 - +0.0 1,383,209 - Psest_1280 0.13 +1.0 1,383,240 - Psest_1280 0.14 +2.6 1,383,364 + Psest_1280 0.20 +0.0 1,383,364 + Psest_1280 0.20 +1.6 1,383,364 + Psest_1280 0.20 +1.6 1,383,364 + Psest_1280 0.20 -2.1 1,383,364 + Psest_1280 0.20 +0.3 1,383,365 - Psest_1280 0.20 +1.0 1,383,365 - Psest_1280 0.20 -0.6 1,383,365 - Psest_1280 0.20 -1.0 1,383,365 - Psest_1280 0.20 +4.9 1,383,365 - Psest_1280 0.20 +3.6 1,383,616 + Psest_1280 0.31 -1.0 1,383,616 + Psest_1280 0.31 +0.3 1,383,617 - Psest_1280 0.31 -1.4 1,383,666 + Psest_1280 0.34 -0.2 1,383,739 + Psest_1280 0.37 +0.0 1,383,748 + Psest_1280 0.37 +0.6 1,383,749 - Psest_1280 0.37 -0.4 1,383,835 + Psest_1280 0.41 +0.3 1,383,835 + Psest_1280 0.41 -0.9 1,383,835 + Psest_1280 0.41 +1.6 1,383,835 + Psest_1280 0.41 -1.0 1,383,836 - Psest_1280 0.41 -0.2
Or see this region's nucleotide sequence