Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1007 and Psest_1008 are separated by 41 nucleotides Psest_1008 and Psest_1009 are separated by 74 nucleotides Psest_1009 and Psest_1010 are separated by 82 nucleotides
Psest_1007: Psest_1007 - Predicted permeases, at 1,092,114 to 1,093,013
_1007
Psest_1008: Psest_1008 - hypothetical protein, at 1,093,055 to 1,093,216
_1008
Psest_1009: Psest_1009 - hypothetical protein, at 1,093,291 to 1,094,049
_1009
Psest_1010: Psest_1010 - Predicted integral membrane protein, at 1,094,132 to 1,095,075
_1010
Position (kb)
1093
1094
1095 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.475 kb on - strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007 at 1093.023 kb on - strand at 1093.023 kb on - strand at 1093.023 kb on - strand at 1093.198 kb on - strand, within Psest_1008 at 1094.126 kb on - strand at 1094.226 kb on + strand at 1094.226 kb on + strand at 1094.226 kb on + strand at 1094.226 kb on + strand at 1094.227 kb on - strand, within Psest_1010 at 1094.227 kb on - strand, within Psest_1010 at 1094.227 kb on - strand, within Psest_1010 at 1094.264 kb on + strand, within Psest_1010 at 1094.264 kb on + strand, within Psest_1010 at 1094.265 kb on - strand, within Psest_1010 at 1094.306 kb on + strand, within Psest_1010 at 1094.306 kb on + strand, within Psest_1010 at 1094.306 kb on + strand, within Psest_1010 at 1094.307 kb on - strand, within Psest_1010 at 1094.307 kb on - strand, within Psest_1010 at 1094.307 kb on - strand, within Psest_1010 at 1094.307 kb on - strand, within Psest_1010 at 1094.310 kb on - strand, within Psest_1010 at 1094.376 kb on - strand, within Psest_1010 at 1094.386 kb on - strand, within Psest_1010 at 1094.418 kb on + strand, within Psest_1010 at 1094.418 kb on + strand, within Psest_1010 at 1094.418 kb on + strand, within Psest_1010 at 1094.418 kb on + strand, within Psest_1010 at 1094.418 kb on + strand, within Psest_1010 at 1094.419 kb on - strand, within Psest_1010 at 1094.419 kb on - strand, within Psest_1010 at 1094.419 kb on - strand, within Psest_1010 at 1094.553 kb on + strand, within Psest_1010 at 1094.553 kb on + strand, within Psest_1010 at 1094.553 kb on + strand, within Psest_1010 at 1094.553 kb on + strand, within Psest_1010 at 1094.553 kb on + strand, within Psest_1010 at 1094.553 kb on + strand, within Psest_1010 at 1094.554 kb on - strand, within Psest_1010 at 1094.731 kb on + strand, within Psest_1010 at 1094.740 kb on + strand, within Psest_1010 at 1094.811 kb on + strand, within Psest_1010 at 1094.811 kb on + strand, within Psest_1010 at 1094.811 kb on + strand, within Psest_1010 at 1094.811 kb on + strand, within Psest_1010 at 1094.812 kb on - strand, within Psest_1010 at 1094.812 kb on - strand, within Psest_1010 at 1094.812 kb on - strand, within Psest_1010 at 1094.812 kb on - strand, within Psest_1010 at 1094.812 kb on - strand, within Psest_1010 at 1094.904 kb on + strand, within Psest_1010 at 1094.988 kb on + strand at 1094.989 kb on - strand at 1094.989 kb on - strand at 1094.989 kb on - strand at 1095.005 kb on + strand at 1095.015 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,092,316 - Psest_1007 0.22 -0.8 1,092,316 - Psest_1007 0.22 -0.8 1,092,316 - Psest_1007 0.22 -1.2 1,092,416 + Psest_1007 0.34 -1.0 1,092,416 + Psest_1007 0.34 +0.1 1,092,416 + Psest_1007 0.34 -1.0 1,092,474 + Psest_1007 0.40 -2.5 1,092,474 + Psest_1007 0.40 -0.8 1,092,474 + Psest_1007 0.40 -2.0 1,092,474 + Psest_1007 0.40 -1.9 1,092,474 + Psest_1007 0.40 +1.5 1,092,475 - Psest_1007 0.40 +1.7 1,092,531 + Psest_1007 0.46 -0.0 1,092,531 + Psest_1007 0.46 -1.9 1,092,532 - Psest_1007 0.46 -0.2 1,092,532 - Psest_1007 0.46 +0.6 1,092,532 - Psest_1007 0.46 -0.0 1,092,532 - Psest_1007 0.46 +0.8 1,092,532 - Psest_1007 0.46 -3.1 1,092,532 - Psest_1007 0.46 -1.3 1,092,532 - Psest_1007 0.46 -0.8 1,092,532 - Psest_1007 0.46 +4.0 1,092,559 + Psest_1007 0.49 -1.7 1,092,559 + Psest_1007 0.49 -0.3 1,092,559 + Psest_1007 0.49 +0.3 1,092,560 - Psest_1007 0.50 +0.8 1,092,560 - Psest_1007 0.50 +0.4 1,093,023 - -2.3 1,093,023 - -1.6 1,093,023 - +0.4 1,093,198 - Psest_1008 0.88 +2.0 1,094,126 - -0.3 1,094,226 + -1.5 1,094,226 + -1.7 1,094,226 + -2.1 1,094,226 + -0.1 1,094,227 - Psest_1010 0.10 -2.4 1,094,227 - Psest_1010 0.10 +2.6 1,094,227 - Psest_1010 0.10 -1.3 1,094,264 + Psest_1010 0.14 +0.2 1,094,264 + Psest_1010 0.14 +1.2 1,094,265 - Psest_1010 0.14 -0.8 1,094,306 + Psest_1010 0.18 +3.1 1,094,306 + Psest_1010 0.18 +3.6 1,094,306 + Psest_1010 0.18 -1.9 1,094,307 - Psest_1010 0.19 -1.6 1,094,307 - Psest_1010 0.19 +0.5 1,094,307 - Psest_1010 0.19 -1.3 1,094,307 - Psest_1010 0.19 +0.4 1,094,310 - Psest_1010 0.19 +0.6 1,094,376 - Psest_1010 0.26 +0.4 1,094,386 - Psest_1010 0.27 -1.6 1,094,418 + Psest_1010 0.30 +0.5 1,094,418 + Psest_1010 0.30 +1.9 1,094,418 + Psest_1010 0.30 +0.4 1,094,418 + Psest_1010 0.30 +0.4 1,094,418 + Psest_1010 0.30 +0.7 1,094,419 - Psest_1010 0.30 -1.3 1,094,419 - Psest_1010 0.30 +0.8 1,094,419 - Psest_1010 0.30 -3.6 1,094,553 + Psest_1010 0.45 -1.6 1,094,553 + Psest_1010 0.45 -0.4 1,094,553 + Psest_1010 0.45 -0.9 1,094,553 + Psest_1010 0.45 +1.8 1,094,553 + Psest_1010 0.45 +1.0 1,094,553 + Psest_1010 0.45 -2.7 1,094,554 - Psest_1010 0.45 -2.1 1,094,731 + Psest_1010 0.63 -2.7 1,094,740 + Psest_1010 0.64 +0.7 1,094,811 + Psest_1010 0.72 -0.9 1,094,811 + Psest_1010 0.72 +1.8 1,094,811 + Psest_1010 0.72 -0.5 1,094,811 + Psest_1010 0.72 +0.7 1,094,812 - Psest_1010 0.72 -3.3 1,094,812 - Psest_1010 0.72 +1.4 1,094,812 - Psest_1010 0.72 +0.8 1,094,812 - Psest_1010 0.72 -0.9 1,094,812 - Psest_1010 0.72 +2.6 1,094,904 + Psest_1010 0.82 +0.9 1,094,988 + +1.2 1,094,989 - +1.0 1,094,989 - +0.4 1,094,989 - +1.2 1,095,005 + +0.4 1,095,015 - +0.7
Or see this region's nucleotide sequence