Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1004 and Psest_1005 overlap by 1 nucleotides Psest_1005 and Psest_1006 are separated by 263 nucleotides Psest_1006 and Psest_1007 are separated by 115 nucleotides
Psest_1004: Psest_1004 - Aspartate/tyrosine/aromatic aminotransferase, at 1,088,935 to 1,090,107
_1004
Psest_1005: Psest_1005 - sugar fermentation stimulation protein, at 1,090,107 to 1,090,814
_1005
Psest_1006: Psest_1006 - dTDP-4-dehydrorhamnose reductase, at 1,091,078 to 1,091,998
_1006
Psest_1007: Psest_1007 - Predicted permeases, at 1,092,114 to 1,093,013
_1007
Position (kb)
1091
1092 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1090.152 kb on + strand at 1090.443 kb on + strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.701 kb on + strand, within Psest_1005 at 1090.943 kb on - strand at 1091.255 kb on + strand, within Psest_1006 at 1091.256 kb on - strand, within Psest_1006 at 1091.256 kb on - strand, within Psest_1006 at 1091.297 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.369 kb on - strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.382 kb on - strand, within Psest_1006 at 1091.382 kb on - strand, within Psest_1006 at 1091.411 kb on + strand, within Psest_1006 at 1091.411 kb on + strand, within Psest_1006 at 1091.412 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.529 kb on - strand, within Psest_1006 at 1091.705 kb on + strand, within Psest_1006 at 1091.706 kb on - strand, within Psest_1006 at 1091.711 kb on + strand, within Psest_1006 at 1091.711 kb on + strand, within Psest_1006 at 1091.712 kb on - strand, within Psest_1006 at 1091.739 kb on - strand, within Psest_1006 at 1091.791 kb on - strand, within Psest_1006 at 1092.053 kb on + strand at 1092.054 kb on - strand at 1092.054 kb on - strand at 1092.054 kb on - strand at 1092.166 kb on - strand at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.475 kb on - strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,090,152 + +0.5 1,090,443 + Psest_1005 0.47 -0.9 1,090,444 - Psest_1005 0.48 -1.3 1,090,444 - Psest_1005 0.48 -0.7 1,090,444 - Psest_1005 0.48 -0.4 1,090,444 - Psest_1005 0.48 -0.2 1,090,444 - Psest_1005 0.48 -0.6 1,090,642 - Psest_1005 0.76 -1.5 1,090,642 - Psest_1005 0.76 -3.3 1,090,699 + Psest_1005 0.84 +1.0 1,090,699 + Psest_1005 0.84 -0.1 1,090,700 - Psest_1005 0.84 -0.4 1,090,700 - Psest_1005 0.84 +0.7 1,090,701 + Psest_1005 0.84 -3.0 1,090,943 - +1.0 1,091,255 + Psest_1006 0.19 +0.9 1,091,256 - Psest_1006 0.19 -0.6 1,091,256 - Psest_1006 0.19 +1.0 1,091,297 + Psest_1006 0.24 +0.3 1,091,368 + Psest_1006 0.31 -0.8 1,091,368 + Psest_1006 0.31 +4.1 1,091,368 + Psest_1006 0.31 +1.3 1,091,369 - Psest_1006 0.32 -0.3 1,091,381 + Psest_1006 0.33 +3.5 1,091,381 + Psest_1006 0.33 +2.0 1,091,381 + Psest_1006 0.33 +1.2 1,091,382 - Psest_1006 0.33 -2.0 1,091,382 - Psest_1006 0.33 -3.8 1,091,411 + Psest_1006 0.36 +0.8 1,091,411 + Psest_1006 0.36 +2.2 1,091,412 - Psest_1006 0.36 -0.1 1,091,448 - Psest_1006 0.40 +2.7 1,091,448 - Psest_1006 0.40 +2.0 1,091,448 - Psest_1006 0.40 +2.1 1,091,448 - Psest_1006 0.40 +0.8 1,091,529 - Psest_1006 0.49 -0.0 1,091,705 + Psest_1006 0.68 +2.5 1,091,706 - Psest_1006 0.68 +2.0 1,091,711 + Psest_1006 0.69 -0.0 1,091,711 + Psest_1006 0.69 +0.5 1,091,712 - Psest_1006 0.69 -2.1 1,091,739 - Psest_1006 0.72 +3.0 1,091,791 - Psest_1006 0.77 -1.9 1,092,053 + -2.0 1,092,054 - -2.5 1,092,054 - -1.3 1,092,054 - -0.0 1,092,166 - +1.6 1,092,316 - Psest_1007 0.22 -0.8 1,092,316 - Psest_1007 0.22 -0.8 1,092,316 - Psest_1007 0.22 -1.2 1,092,416 + Psest_1007 0.34 -1.0 1,092,416 + Psest_1007 0.34 +0.1 1,092,416 + Psest_1007 0.34 -1.0 1,092,474 + Psest_1007 0.40 -2.5 1,092,474 + Psest_1007 0.40 -0.8 1,092,474 + Psest_1007 0.40 -2.0 1,092,474 + Psest_1007 0.40 -1.9 1,092,474 + Psest_1007 0.40 +1.5 1,092,475 - Psest_1007 0.40 +1.7 1,092,531 + Psest_1007 0.46 -0.0 1,092,531 + Psest_1007 0.46 -1.9 1,092,532 - Psest_1007 0.46 -0.2 1,092,532 - Psest_1007 0.46 +0.6 1,092,532 - Psest_1007 0.46 -0.0 1,092,532 - Psest_1007 0.46 +0.8 1,092,532 - Psest_1007 0.46 -3.1 1,092,532 - Psest_1007 0.46 -1.3 1,092,532 - Psest_1007 0.46 -0.8 1,092,532 - Psest_1007 0.46 +4.0 1,092,559 + Psest_1007 0.49 -1.7 1,092,559 + Psest_1007 0.49 -0.3 1,092,559 + Psest_1007 0.49 +0.3 1,092,560 - Psest_1007 0.50 +0.8 1,092,560 - Psest_1007 0.50 +0.4
Or see this region's nucleotide sequence