Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1002 and Psest_1003 are separated by 89 nucleotides Psest_1003 and Psest_1004 are separated by 191 nucleotides Psest_1004 and Psest_1005 overlap by 1 nucleotides Psest_1005 and Psest_1006 are separated by 263 nucleotides
Psest_1002: Psest_1002 - glutamyl-queuosine tRNA(Asp) synthetase, at 1,087,302 to 1,088,207
_1002
Psest_1003: Psest_1003 - RNA polymerase-binding protein DksA, at 1,088,297 to 1,088,743
_1003
Psest_1004: Psest_1004 - Aspartate/tyrosine/aromatic aminotransferase, at 1,088,935 to 1,090,107
_1004
Psest_1005: Psest_1005 - sugar fermentation stimulation protein, at 1,090,107 to 1,090,814
_1005
Psest_1006: Psest_1006 - dTDP-4-dehydrorhamnose reductase, at 1,091,078 to 1,091,998
_1006
Position (kb)
1088
1089
1090
1091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1087.990 kb on + strand, within Psest_1002 at 1087.991 kb on - strand, within Psest_1002 at 1087.991 kb on - strand, within Psest_1002 at 1088.101 kb on + strand, within Psest_1002 at 1088.102 kb on - strand, within Psest_1002 at 1088.102 kb on - strand, within Psest_1002 at 1088.686 kb on - strand, within Psest_1003 at 1088.686 kb on - strand, within Psest_1003 at 1088.901 kb on - strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1089.128 kb on - strand, within Psest_1004 at 1089.130 kb on - strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.202 kb on - strand, within Psest_1004 at 1089.202 kb on - strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.569 kb on - strand, within Psest_1004 at 1089.600 kb on - strand, within Psest_1004 at 1089.637 kb on + strand, within Psest_1004 at 1089.637 kb on + strand, within Psest_1004 at 1089.637 kb on + strand, within Psest_1004 at 1089.638 kb on - strand, within Psest_1004 at 1089.638 kb on - strand, within Psest_1004 at 1089.638 kb on - strand, within Psest_1004 at 1089.724 kb on + strand, within Psest_1004 at 1089.725 kb on - strand, within Psest_1004 at 1089.725 kb on - strand, within Psest_1004 at 1089.867 kb on + strand, within Psest_1004 at 1089.896 kb on - strand, within Psest_1004 at 1089.896 kb on - strand, within Psest_1004 at 1089.896 kb on - strand, within Psest_1004 at 1089.902 kb on - strand, within Psest_1004 at 1089.902 kb on - strand, within Psest_1004 at 1090.034 kb on - strand at 1090.034 kb on - strand at 1090.152 kb on + strand at 1090.443 kb on + strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.701 kb on + strand, within Psest_1005 at 1090.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,087,990 + Psest_1002 0.76 +0.9 1,087,991 - Psest_1002 0.76 -0.0 1,087,991 - Psest_1002 0.76 -2.7 1,088,101 + Psest_1002 0.88 -2.6 1,088,102 - Psest_1002 0.88 -2.3 1,088,102 - Psest_1002 0.88 +1.1 1,088,686 - Psest_1003 0.87 -1.2 1,088,686 - Psest_1003 0.87 -1.5 1,088,901 - -0.0 1,088,947 + +3.4 1,088,947 + -1.7 1,088,947 + +1.0 1,088,947 + +0.0 1,088,947 + -0.5 1,088,947 + +0.4 1,088,947 + +0.5 1,088,947 + -1.6 1,088,948 - -1.3 1,088,948 - +0.8 1,088,948 - -1.2 1,088,948 - -0.2 1,089,128 - Psest_1004 0.16 -1.1 1,089,130 - Psest_1004 0.17 -0.0 1,089,197 + Psest_1004 0.22 +0.1 1,089,197 + Psest_1004 0.22 -0.9 1,089,197 + Psest_1004 0.22 -0.0 1,089,198 - Psest_1004 0.22 -0.5 1,089,198 - Psest_1004 0.22 -2.3 1,089,198 - Psest_1004 0.22 +0.6 1,089,198 - Psest_1004 0.22 -1.2 1,089,202 - Psest_1004 0.23 +0.3 1,089,202 - Psest_1004 0.23 -2.3 1,089,553 + Psest_1004 0.53 -0.2 1,089,553 + Psest_1004 0.53 -2.8 1,089,553 + Psest_1004 0.53 -1.4 1,089,553 + Psest_1004 0.53 -0.0 1,089,554 - Psest_1004 0.53 -1.7 1,089,554 - Psest_1004 0.53 +2.9 1,089,554 - Psest_1004 0.53 -0.6 1,089,554 - Psest_1004 0.53 -2.0 1,089,569 - Psest_1004 0.54 -0.6 1,089,600 - Psest_1004 0.57 -0.1 1,089,637 + Psest_1004 0.60 -1.4 1,089,637 + Psest_1004 0.60 -2.9 1,089,637 + Psest_1004 0.60 -2.1 1,089,638 - Psest_1004 0.60 +0.7 1,089,638 - Psest_1004 0.60 -0.5 1,089,638 - Psest_1004 0.60 +0.6 1,089,724 + Psest_1004 0.67 -0.0 1,089,725 - Psest_1004 0.67 +1.4 1,089,725 - Psest_1004 0.67 +0.7 1,089,867 + Psest_1004 0.79 -0.1 1,089,896 - Psest_1004 0.82 -2.6 1,089,896 - Psest_1004 0.82 -1.1 1,089,896 - Psest_1004 0.82 +2.0 1,089,902 - Psest_1004 0.82 +1.3 1,089,902 - Psest_1004 0.82 -2.0 1,090,034 - +0.5 1,090,034 - +0.9 1,090,152 + +0.5 1,090,443 + Psest_1005 0.47 -0.9 1,090,444 - Psest_1005 0.48 -1.3 1,090,444 - Psest_1005 0.48 -0.7 1,090,444 - Psest_1005 0.48 -0.4 1,090,444 - Psest_1005 0.48 -0.2 1,090,444 - Psest_1005 0.48 -0.6 1,090,642 - Psest_1005 0.76 -1.5 1,090,642 - Psest_1005 0.76 -3.3 1,090,699 + Psest_1005 0.84 +1.0 1,090,699 + Psest_1005 0.84 -0.1 1,090,700 - Psest_1005 0.84 -0.4 1,090,700 - Psest_1005 0.84 +0.7 1,090,701 + Psest_1005 0.84 -3.0 1,090,943 - +1.0
Or see this region's nucleotide sequence