Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0731 and Psest_0732 are separated by 154 nucleotides Psest_0732 and Psest_0733 overlap by 4 nucleotides
Psest_0731: Psest_0731 - Serine/threonine protein kinase involved in cell cycle control, at 800,735 to 801,625
_0731
Psest_0732: Psest_0732 - efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family, at 801,780 to 803,252
_0732
Psest_0733: Psest_0733 - Cation/multidrug efflux pump, at 803,249 to 806,293
_0733
Position (kb)
801
802
803
804 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 800.826 kb on + strand, within Psest_0731 at 800.826 kb on + strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.827 kb on - strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.828 kb on + strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.829 kb on - strand, within Psest_0731 at 800.870 kb on + strand, within Psest_0731 at 800.871 kb on - strand, within Psest_0731 at 800.871 kb on - strand, within Psest_0731 at 800.871 kb on - strand, within Psest_0731 at 800.871 kb on - strand, within Psest_0731 at 800.912 kb on + strand, within Psest_0731 at 800.938 kb on + strand, within Psest_0731 at 800.939 kb on - strand, within Psest_0731 at 800.939 kb on - strand, within Psest_0731 at 801.240 kb on + strand, within Psest_0731 at 801.240 kb on + strand, within Psest_0731 at 801.368 kb on + strand, within Psest_0731 at 801.369 kb on - strand, within Psest_0731 at 801.689 kb on + strand at 801.690 kb on - strand at 801.856 kb on + strand at 801.856 kb on + strand at 801.857 kb on - strand at 801.857 kb on - strand at 801.857 kb on - strand at 801.857 kb on - strand at 801.857 kb on - strand at 801.857 kb on - strand at 801.857 kb on - strand at 801.857 kb on - strand at 802.120 kb on + strand, within Psest_0732 at 802.121 kb on - strand, within Psest_0732 at 802.121 kb on - strand, within Psest_0732 at 802.121 kb on - strand, within Psest_0732 at 803.031 kb on + strand, within Psest_0732 at 803.031 kb on + strand, within Psest_0732 at 803.032 kb on - strand, within Psest_0732 at 803.032 kb on - strand, within Psest_0732 at 803.402 kb on + strand at 803.478 kb on - strand at 803.478 kb on - strand at 803.784 kb on + strand, within Psest_0733 at 803.784 kb on + strand, within Psest_0733 at 803.785 kb on - strand, within Psest_0733 at 803.785 kb on - strand, within Psest_0733 at 803.785 kb on - strand, within Psest_0733 at 803.785 kb on - strand, within Psest_0733 at 803.964 kb on + strand, within Psest_0733 at 803.965 kb on - strand, within Psest_0733 at 804.221 kb on + strand, within Psest_0733 at 804.221 kb on + strand, within Psest_0733
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 800,826 + Psest_0731 0.10 +1.2 800,826 + Psest_0731 0.10 +0.1 800,827 - Psest_0731 0.10 +0.5 800,827 - Psest_0731 0.10 +0.4 800,827 - Psest_0731 0.10 -1.1 800,827 - Psest_0731 0.10 -0.2 800,827 - Psest_0731 0.10 +0.5 800,827 - Psest_0731 0.10 -1.0 800,827 - Psest_0731 0.10 +1.0 800,827 - Psest_0731 0.10 +1.5 800,827 - Psest_0731 0.10 -2.4 800,828 + Psest_0731 0.10 +1.4 800,828 + Psest_0731 0.10 +1.6 800,828 + Psest_0731 0.10 +0.7 800,828 + Psest_0731 0.10 -0.8 800,828 + Psest_0731 0.10 +3.5 800,828 + Psest_0731 0.10 +0.2 800,828 + Psest_0731 0.10 -0.4 800,828 + Psest_0731 0.10 -1.1 800,828 + Psest_0731 0.10 +0.9 800,828 + Psest_0731 0.10 +1.9 800,828 + Psest_0731 0.10 +2.0 800,828 + Psest_0731 0.10 +2.1 800,828 + Psest_0731 0.10 +2.1 800,828 + Psest_0731 0.10 -0.2 800,828 + Psest_0731 0.10 -1.7 800,828 + Psest_0731 0.10 +1.2 800,828 + Psest_0731 0.10 -2.4 800,828 + Psest_0731 0.10 +1.7 800,828 + Psest_0731 0.10 +0.0 800,828 + Psest_0731 0.10 +0.4 800,828 + Psest_0731 0.10 -0.5 800,829 - Psest_0731 0.11 -2.9 800,829 - Psest_0731 0.11 +2.6 800,829 - Psest_0731 0.11 -0.0 800,829 - Psest_0731 0.11 +2.2 800,829 - Psest_0731 0.11 -0.5 800,829 - Psest_0731 0.11 +1.5 800,829 - Psest_0731 0.11 -2.0 800,829 - Psest_0731 0.11 -1.0 800,829 - Psest_0731 0.11 +0.5 800,829 - Psest_0731 0.11 +0.9 800,829 - Psest_0731 0.11 +0.9 800,829 - Psest_0731 0.11 -0.8 800,829 - Psest_0731 0.11 -0.5 800,829 - Psest_0731 0.11 -1.0 800,829 - Psest_0731 0.11 +2.3 800,829 - Psest_0731 0.11 -0.8 800,829 - Psest_0731 0.11 -0.4 800,829 - Psest_0731 0.11 +0.6 800,829 - Psest_0731 0.11 +1.7 800,829 - Psest_0731 0.11 -1.8 800,829 - Psest_0731 0.11 +0.9 800,829 - Psest_0731 0.11 +1.2 800,870 + Psest_0731 0.15 -2.4 800,871 - Psest_0731 0.15 -1.5 800,871 - Psest_0731 0.15 +1.0 800,871 - Psest_0731 0.15 +1.7 800,871 - Psest_0731 0.15 -0.2 800,912 + Psest_0731 0.20 +0.7 800,938 + Psest_0731 0.23 -3.0 800,939 - Psest_0731 0.23 -3.9 800,939 - Psest_0731 0.23 +1.5 801,240 + Psest_0731 0.57 +1.3 801,240 + Psest_0731 0.57 -3.0 801,368 + Psest_0731 0.71 +3.2 801,369 - Psest_0731 0.71 +2.7 801,689 + -0.4 801,690 - -1.1 801,856 + -2.0 801,856 + +2.6 801,857 - -0.0 801,857 - -1.1 801,857 - +1.0 801,857 - -2.5 801,857 - +2.3 801,857 - -0.8 801,857 - +0.0 801,857 - -0.2 802,120 + Psest_0732 0.23 -0.1 802,121 - Psest_0732 0.23 -0.3 802,121 - Psest_0732 0.23 +0.0 802,121 - Psest_0732 0.23 -3.1 803,031 + Psest_0732 0.85 +0.9 803,031 + Psest_0732 0.85 -2.0 803,032 - Psest_0732 0.85 -1.1 803,032 - Psest_0732 0.85 -3.7 803,402 + +6.1 803,478 - -3.0 803,478 - -0.5 803,784 + Psest_0733 0.18 -2.1 803,784 + Psest_0733 0.18 -2.0 803,785 - Psest_0733 0.18 +1.7 803,785 - Psest_0733 0.18 -1.1 803,785 - Psest_0733 0.18 +1.7 803,785 - Psest_0733 0.18 +1.8 803,964 + Psest_0733 0.23 +1.9 803,965 - Psest_0733 0.24 +0.6 804,221 + Psest_0733 0.32 -1.9 804,221 + Psest_0733 0.32 +0.7
Or see this region's nucleotide sequence