Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0481 and Psest_0482 are separated by 166 nucleotides Psest_0482 and Psest_0483 are separated by 63 nucleotides
Psest_0481: Psest_0481 - Predicted phosphohydrolases, at 530,851 to 531,666
_0481
Psest_0482: Psest_0482 - yqiA-like hydrolase, affects the cell envelope <small>(from data)</small>, at 531,833 to 532,438
_0482
Psest_0483: Psest_0483 - DNA topoisomerase IV, B subunit, proteobacterial, at 532,502 to 534,481
_0483
Position (kb) 531
532
533 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 530.955 kb on + strand, within Psest_0481 at 531.662 kb on - strand at 531.738 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.746 kb on + strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.747 kb on - strand at 531.802 kb on + strand at 531.802 kb on + strand at 531.803 kb on - strand at 531.803 kb on - strand at 531.820 kb on - strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.860 kb on + strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.861 kb on - strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on + strand at 531.862 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.863 kb on - strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.864 kb on + strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 531.865 kb on - strand at 532.056 kb on - strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.058 kb on + strand, within Psest_0482 at 532.142 kb on + strand, within Psest_0482 at 532.166 kb on + strand, within Psest_0482 at 532.292 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.313 kb on + strand, within Psest_0482 at 532.333 kb on + strand, within Psest_0482 at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand at 532.576 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 530,955 + Psest_0481 0.13 +2.1 531,662 - -0.7 531,738 + -2.9 531,746 + -0.3 531,746 + -2.5 531,746 + -1.9 531,746 + +2.0 531,746 + +0.2 531,746 + -0.8 531,747 - +0.6 531,747 - +1.2 531,747 - -3.0 531,747 - +0.7 531,747 - -1.0 531,747 - +3.2 531,802 + -1.7 531,802 + +1.6 531,803 - +0.3 531,803 - -2.7 531,820 - +2.4 531,860 + -0.7 531,860 + -0.2 531,860 + +2.5 531,860 + +2.2 531,860 + -0.4 531,860 + +2.2 531,860 + +1.3 531,860 + +2.5 531,860 + -2.1 531,861 - -1.4 531,861 - +0.7 531,861 - +1.0 531,861 - +1.1 531,861 - -1.7 531,861 - +1.7 531,861 - -0.4 531,862 + -2.4 531,862 + +1.2 531,862 + -0.9 531,862 + -0.3 531,862 + -0.1 531,862 + -1.8 531,862 + +1.6 531,862 + +2.7 531,862 + -1.1 531,862 + +0.1 531,862 + +0.5 531,862 + +1.1 531,862 - -0.4 531,863 - -3.2 531,863 - -2.6 531,863 - -0.3 531,863 - -0.2 531,863 - +0.7 531,863 - -3.8 531,863 - +2.0 531,863 - +0.1 531,863 - -0.2 531,863 - +0.0 531,863 - -2.2 531,863 - +0.8 531,863 - +0.1 531,864 + +0.8 531,864 + +2.8 531,864 + +0.1 531,864 + +0.3 531,864 + +0.5 531,864 + +0.4 531,864 + +1.4 531,864 + -1.8 531,864 + +1.3 531,864 + +0.1 531,864 + -0.5 531,864 + +2.5 531,865 - -0.8 531,865 - +1.0 531,865 - +0.3 531,865 - +0.3 531,865 - -2.7 531,865 - -1.2 531,865 - +0.9 531,865 - -0.2 531,865 - -2.9 531,865 - -2.9 531,865 - +0.0 531,865 - +1.3 531,865 - +1.6 532,056 - Psest_0482 0.37 -2.4 532,058 + Psest_0482 0.37 -2.0 532,058 + Psest_0482 0.37 +2.9 532,058 + Psest_0482 0.37 +0.2 532,142 + Psest_0482 0.51 -1.6 532,166 + Psest_0482 0.55 -0.2 532,292 + Psest_0482 0.76 +1.1 532,313 + Psest_0482 0.79 -1.2 532,313 + Psest_0482 0.79 +1.1 532,313 + Psest_0482 0.79 +0.5 532,333 + Psest_0482 0.83 +1.5 532,576 + -0.9 532,576 + -1.1 532,576 + +1.1 532,576 + -2.4 532,576 + -0.6 532,576 + +0.4 532,576 + +0.4 532,576 + +0.2 532,576 + -3.1 532,576 + -0.4
Or see this region's nucleotide sequence