Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3151 and Psest_3153 are separated by 278 nucleotides Psest_3153 and Psest_3154 overlap by 10 nucleotides Psest_3154 and Psest_3155 are separated by 639 nucleotides
Psest_3151: Psest_3151 - hypothetical protein, at 3,321,918 to 3,322,388
_3151
Psest_3153: Psest_3153 - hypothetical protein, at 3,322,667 to 3,322,885
_3153
Psest_3154: Psest_3154 - hypothetical protein, at 3,322,876 to 3,324,540
_3154
Psest_3155: Psest_3155 - hypothetical protein, at 3,325,180 to 3,325,464
_3155
Position (kb)
3322
3323
3324
3325 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3321.932 kb on - strand at 3322.140 kb on + strand, within Psest_3151 at 3322.141 kb on - strand, within Psest_3151 at 3322.143 kb on - strand, within Psest_3151 at 3322.181 kb on + strand, within Psest_3151 at 3322.269 kb on + strand, within Psest_3151 at 3322.386 kb on + strand at 3323.145 kb on - strand, within Psest_3154 at 3323.206 kb on - strand, within Psest_3154 at 3323.239 kb on + strand, within Psest_3154 at 3323.375 kb on + strand, within Psest_3154 at 3323.375 kb on + strand, within Psest_3154 at 3323.375 kb on + strand, within Psest_3154 at 3323.376 kb on - strand, within Psest_3154 at 3323.376 kb on - strand, within Psest_3154 at 3323.376 kb on - strand, within Psest_3154 at 3323.376 kb on - strand, within Psest_3154 at 3323.435 kb on + strand, within Psest_3154 at 3323.521 kb on - strand, within Psest_3154 at 3323.616 kb on + strand, within Psest_3154 at 3323.616 kb on + strand, within Psest_3154 at 3323.675 kb on - strand, within Psest_3154 at 3323.675 kb on - strand, within Psest_3154 at 3323.699 kb on + strand, within Psest_3154 at 3323.700 kb on - strand, within Psest_3154 at 3323.780 kb on - strand, within Psest_3154 at 3323.826 kb on + strand, within Psest_3154 at 3323.826 kb on + strand, within Psest_3154 at 3323.826 kb on + strand, within Psest_3154 at 3323.884 kb on - strand, within Psest_3154 at 3323.973 kb on + strand, within Psest_3154 at 3324.013 kb on + strand, within Psest_3154 at 3324.014 kb on - strand, within Psest_3154 at 3324.014 kb on - strand, within Psest_3154 at 3324.014 kb on - strand, within Psest_3154 at 3324.071 kb on - strand, within Psest_3154 at 3324.071 kb on - strand, within Psest_3154 at 3324.071 kb on - strand, within Psest_3154 at 3324.071 kb on - strand, within Psest_3154 at 3324.071 kb on - strand, within Psest_3154 at 3324.071 kb on - strand, within Psest_3154 at 3324.071 kb on - strand, within Psest_3154 at 3324.073 kb on - strand, within Psest_3154 at 3324.228 kb on - strand, within Psest_3154 at 3324.375 kb on - strand at 3324.388 kb on - strand at 3324.426 kb on + strand at 3324.465 kb on + strand at 3324.465 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.552 kb on + strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.553 kb on - strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.554 kb on + strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.555 kb on - strand at 3324.806 kb on + strand at 3324.806 kb on + strand at 3324.806 kb on + strand at 3324.810 kb on + strand at 3324.810 kb on + strand at 3324.810 kb on + strand at 3324.810 kb on + strand at 3324.811 kb on - strand at 3324.812 kb on + strand at 3324.812 kb on + strand at 3324.812 kb on + strand at 3324.812 kb on + strand at 3324.867 kb on - strand at 3324.915 kb on + strand at 3324.916 kb on - strand at 3324.942 kb on + strand at 3324.942 kb on + strand at 3324.942 kb on + strand at 3324.942 kb on + strand at 3324.953 kb on + strand at 3324.995 kb on + strand at 3324.995 kb on + strand at 3325.151 kb on - strand at 3325.164 kb on + strand at 3325.334 kb on + strand, within Psest_3155 at 3325.334 kb on + strand, within Psest_3155 at 3325.335 kb on - strand, within Psest_3155 at 3325.335 kb on - strand, within Psest_3155 at 3325.335 kb on - strand, within Psest_3155 at 3325.537 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 3,321,932 - -0.4 3,322,140 + Psest_3151 0.47 +0.4 3,322,141 - Psest_3151 0.47 +1.2 3,322,143 - Psest_3151 0.48 -1.7 3,322,181 + Psest_3151 0.56 -2.5 3,322,269 + Psest_3151 0.75 -0.3 3,322,386 + +5.7 3,323,145 - Psest_3154 0.16 -0.8 3,323,206 - Psest_3154 0.20 +0.6 3,323,239 + Psest_3154 0.22 -0.9 3,323,375 + Psest_3154 0.30 +2.0 3,323,375 + Psest_3154 0.30 -0.0 3,323,375 + Psest_3154 0.30 +0.8 3,323,376 - Psest_3154 0.30 -2.0 3,323,376 - Psest_3154 0.30 +0.1 3,323,376 - Psest_3154 0.30 -1.6 3,323,376 - Psest_3154 0.30 -0.6 3,323,435 + Psest_3154 0.34 -0.6 3,323,521 - Psest_3154 0.39 +0.4 3,323,616 + Psest_3154 0.44 -0.6 3,323,616 + Psest_3154 0.44 -0.1 3,323,675 - Psest_3154 0.48 +1.3 3,323,675 - Psest_3154 0.48 +0.2 3,323,699 + Psest_3154 0.49 -3.4 3,323,700 - Psest_3154 0.49 +0.5 3,323,780 - Psest_3154 0.54 -2.5 3,323,826 + Psest_3154 0.57 +0.7 3,323,826 + Psest_3154 0.57 -0.5 3,323,826 + Psest_3154 0.57 -0.2 3,323,884 - Psest_3154 0.61 -0.9 3,323,973 + Psest_3154 0.66 +0.4 3,324,013 + Psest_3154 0.68 -2.7 3,324,014 - Psest_3154 0.68 -2.9 3,324,014 - Psest_3154 0.68 -0.3 3,324,014 - Psest_3154 0.68 -0.3 3,324,071 - Psest_3154 0.72 -0.6 3,324,071 - Psest_3154 0.72 -1.1 3,324,071 - Psest_3154 0.72 -0.2 3,324,071 - Psest_3154 0.72 +3.5 3,324,071 - Psest_3154 0.72 -1.9 3,324,071 - Psest_3154 0.72 +0.9 3,324,071 - Psest_3154 0.72 -0.9 3,324,073 - Psest_3154 0.72 +1.5 3,324,228 - Psest_3154 0.81 -0.3 3,324,375 - -3.8 3,324,388 - -2.1 3,324,426 + -2.8 3,324,465 + -2.2 3,324,465 + +1.8 3,324,552 + -3.0 3,324,552 + -0.8 3,324,552 + -1.0 3,324,552 + -2.2 3,324,552 + +0.1 3,324,552 + -0.2 3,324,552 + -1.7 3,324,552 + -2.9 3,324,552 + +0.9 3,324,552 + -0.3 3,324,552 + +0.3 3,324,553 - -2.2 3,324,553 - +1.7 3,324,553 - +1.4 3,324,553 - +0.2 3,324,553 - +0.3 3,324,553 - -0.3 3,324,553 - +0.7 3,324,553 - +0.2 3,324,553 - +1.1 3,324,553 - +1.9 3,324,553 - -0.8 3,324,553 - -0.6 3,324,554 + -2.6 3,324,554 + -3.1 3,324,554 + +1.0 3,324,554 + -0.3 3,324,554 + +1.3 3,324,554 + +1.7 3,324,554 + +0.8 3,324,554 + +0.3 3,324,554 + -2.6 3,324,554 + +2.2 3,324,554 + -1.7 3,324,554 + +1.2 3,324,554 + +0.9 3,324,554 + +2.6 3,324,554 + +0.5 3,324,555 - -0.6 3,324,555 - -2.8 3,324,555 - +4.0 3,324,555 - +1.3 3,324,555 - +3.3 3,324,555 - +0.5 3,324,555 - +2.9 3,324,555 - +3.7 3,324,555 - -2.8 3,324,555 - +0.3 3,324,555 - +2.8 3,324,555 - -0.1 3,324,555 - -1.2 3,324,555 - +2.1 3,324,555 - +0.4 3,324,806 + +1.1 3,324,806 + +0.7 3,324,806 + +0.8 3,324,810 + -0.3 3,324,810 + -1.0 3,324,810 + +0.9 3,324,810 + -3.5 3,324,811 - -1.5 3,324,812 + +1.3 3,324,812 + +0.4 3,324,812 + +1.3 3,324,812 + +0.6 3,324,867 - +0.0 3,324,915 + -3.5 3,324,916 - +0.9 3,324,942 + -2.1 3,324,942 + -0.0 3,324,942 + +2.2 3,324,942 + -1.1 3,324,953 + +0.0 3,324,995 + +1.0 3,324,995 + +0.7 3,325,151 - +0.3 3,325,164 + -0.1 3,325,334 + Psest_3155 0.54 +0.7 3,325,334 + Psest_3155 0.54 -3.1 3,325,335 - Psest_3155 0.54 -0.6 3,325,335 - Psest_3155 0.54 +0.6 3,325,335 - Psest_3155 0.54 -3.3 3,325,537 + +0.3
Or see this region's nucleotide sequence