Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0288 and Psest_0289 are separated by 133 nucleotides Psest_0289 and Psest_0290 are separated by 67 nucleotides Psest_0290 and Psest_0291 overlap by 1 nucleotides
Psest_0288: Psest_0288 - putative DNA modification/repair radical SAM protein, at 303,412 to 304,623
_0288
Psest_0289: Psest_0289 - Predicted membrane protein, at 304,757 to 305,152
_0289
Psest_0290: Psest_0290 - dihydroorotase, multifunctional complex type, at 305,220 to 306,494
_0290
Psest_0291: Psest_0291 - aspartate carbamoyltransferase, at 306,494 to 307,510
_0291
Position (kb) 305
306
307 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 304.268 kb on + strand, within Psest_0288 at 304.269 kb on - strand, within Psest_0288 at 304.269 kb on - strand, within Psest_0288 at 304.410 kb on - strand, within Psest_0288 at 304.421 kb on + strand, within Psest_0288 at 304.422 kb on - strand, within Psest_0288 at 304.422 kb on - strand, within Psest_0288 at 304.577 kb on + strand at 304.577 kb on + strand at 304.577 kb on + strand at 304.577 kb on + strand at 304.578 kb on - strand at 304.639 kb on - strand at 304.639 kb on - strand at 304.639 kb on - strand at 304.639 kb on - strand at 304.651 kb on + strand at 304.651 kb on + strand at 304.652 kb on - strand at 304.652 kb on - strand at 304.657 kb on - strand at 304.657 kb on - strand at 304.774 kb on + strand at 304.774 kb on + strand at 304.775 kb on - strand at 304.791 kb on - strand at 304.832 kb on + strand, within Psest_0289 at 304.832 kb on + strand, within Psest_0289 at 304.832 kb on + strand, within Psest_0289 at 304.833 kb on - strand, within Psest_0289 at 304.835 kb on + strand, within Psest_0289 at 304.970 kb on + strand, within Psest_0289 at 304.971 kb on - strand, within Psest_0289 at 304.987 kb on - strand, within Psest_0289 at 305.017 kb on - strand, within Psest_0289 at 305.017 kb on - strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.028 kb on - strand, within Psest_0289 at 305.028 kb on - strand, within Psest_0289 at 305.028 kb on - strand, within Psest_0289 at 305.057 kb on + strand, within Psest_0289 at 305.097 kb on - strand, within Psest_0289 at 305.188 kb on + strand at 305.188 kb on + strand at 305.189 kb on - strand at 305.189 kb on - strand at 305.659 kb on - strand, within Psest_0290 at 305.987 kb on - strand, within Psest_0290 at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.732 kb on + strand, within Psest_0291 at 306.732 kb on + strand, within Psest_0291 at 306.741 kb on + strand, within Psest_0291 at 307.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 304,268 + Psest_0288 0.71 +1.3 304,269 - Psest_0288 0.71 +1.3 304,269 - Psest_0288 0.71 -2.1 304,410 - Psest_0288 0.82 +0.2 304,421 + Psest_0288 0.83 -0.6 304,422 - Psest_0288 0.83 -2.6 304,422 - Psest_0288 0.83 -0.2 304,577 + +1.6 304,577 + +1.6 304,577 + +1.6 304,577 + +0.8 304,578 - -2.9 304,639 - -1.5 304,639 - +2.8 304,639 - +0.7 304,639 - +3.1 304,651 + +0.1 304,651 + +1.0 304,652 - -0.7 304,652 - +1.4 304,657 - +2.6 304,657 - +0.4 304,774 + +0.7 304,774 + +0.7 304,775 - -2.8 304,791 - +0.2 304,832 + Psest_0289 0.19 -0.6 304,832 + Psest_0289 0.19 +0.1 304,832 + Psest_0289 0.19 +0.3 304,833 - Psest_0289 0.19 +0.0 304,835 + Psest_0289 0.20 -0.8 304,970 + Psest_0289 0.54 +0.3 304,971 - Psest_0289 0.54 +0.6 304,987 - Psest_0289 0.58 -1.4 305,017 - Psest_0289 0.66 +0.4 305,017 - Psest_0289 0.66 -0.9 305,027 + Psest_0289 0.68 -3.7 305,027 + Psest_0289 0.68 -3.3 305,027 + Psest_0289 0.68 -1.8 305,027 + Psest_0289 0.68 +1.7 305,027 + Psest_0289 0.68 -1.4 305,027 + Psest_0289 0.68 -2.1 305,027 + Psest_0289 0.68 -3.0 305,027 + Psest_0289 0.68 +1.1 305,027 + Psest_0289 0.68 +0.5 305,027 + Psest_0289 0.68 +0.4 305,028 - Psest_0289 0.68 -0.3 305,028 - Psest_0289 0.68 -2.5 305,028 - Psest_0289 0.68 -0.7 305,057 + Psest_0289 0.76 -1.4 305,097 - Psest_0289 0.86 -0.9 305,188 + -2.2 305,188 + -1.0 305,189 - -0.7 305,189 - -1.4 305,659 - Psest_0290 0.34 +0.5 305,987 - Psest_0290 0.60 +2.1 306,595 - +0.1 306,595 - +1.1 306,595 - +0.1 306,732 + Psest_0291 0.23 +2.1 306,732 + Psest_0291 0.23 +1.1 306,741 + Psest_0291 0.24 +1.1 307,482 - -0.2
Or see this region's nucleotide sequence