Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2513 and Psest_2514 are separated by 102 nucleotides Psest_2514 and Psest_2515 are separated by 147 nucleotides Psest_2515 and Psest_2516 overlap by 4 nucleotides
Psest_2513: Psest_2513 - Predicted membrane protein, at 2,691,642 to 2,692,055
_2513
Psest_2514: Psest_2514 - Predicted membrane protein, at 2,692,158 to 2,692,637
_2514
Psest_2515: Psest_2515 - cytochrome c oxidase, subunit II, at 2,692,785 to 2,693,675
_2515
Psest_2516: Psest_2516 - cytochrome c oxidase, subunit I, at 2,693,672 to 2,696,197
_2516
Position (kb)
2692
2693
2694 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2691.975 kb on + strand, within Psest_2513 at 2691.976 kb on - strand, within Psest_2513 at 2691.976 kb on - strand, within Psest_2513 at 2691.976 kb on - strand, within Psest_2513 at 2692.517 kb on - strand, within Psest_2514 at 2692.607 kb on + strand at 2692.607 kb on + strand at 2692.657 kb on + strand at 2692.657 kb on + strand at 2692.657 kb on + strand at 2692.657 kb on + strand at 2692.658 kb on - strand at 2692.840 kb on + strand at 2692.869 kb on + strand at 2692.869 kb on + strand at 2692.869 kb on + strand at 2692.870 kb on - strand at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.896 kb on + strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2692.897 kb on - strand, within Psest_2515 at 2693.047 kb on + strand, within Psest_2515 at 2693.107 kb on + strand, within Psest_2515 at 2693.910 kb on - strand at 2693.910 kb on - strand at 2693.910 kb on - strand at 2693.987 kb on + strand, within Psest_2516 at 2694.024 kb on + strand, within Psest_2516 at 2694.053 kb on + strand, within Psest_2516 at 2694.059 kb on + strand, within Psest_2516 at 2694.060 kb on - strand, within Psest_2516 at 2694.131 kb on + strand, within Psest_2516 at 2694.131 kb on + strand, within Psest_2516 at 2694.131 kb on + strand, within Psest_2516 at 2694.131 kb on + strand, within Psest_2516 at 2694.131 kb on + strand, within Psest_2516 at 2694.132 kb on - strand, within Psest_2516 at 2694.132 kb on - strand, within Psest_2516 at 2694.132 kb on - strand, within Psest_2516 at 2694.219 kb on + strand, within Psest_2516 at 2694.296 kb on + strand, within Psest_2516 at 2694.297 kb on - strand, within Psest_2516 at 2694.297 kb on - strand, within Psest_2516 at 2694.305 kb on + strand, within Psest_2516 at 2694.306 kb on - strand, within Psest_2516 at 2694.306 kb on - strand, within Psest_2516 at 2694.306 kb on - strand, within Psest_2516 at 2694.476 kb on + strand, within Psest_2516 at 2694.476 kb on + strand, within Psest_2516 at 2694.476 kb on + strand, within Psest_2516 at 2694.476 kb on + strand, within Psest_2516 at 2694.476 kb on + strand, within Psest_2516 at 2694.476 kb on + strand, within Psest_2516 at 2694.476 kb on + strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516 at 2694.477 kb on - strand, within Psest_2516
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 2,691,975 + Psest_2513 0.80 -2.2 2,691,976 - Psest_2513 0.81 +1.6 2,691,976 - Psest_2513 0.81 +0.0 2,691,976 - Psest_2513 0.81 -0.9 2,692,517 - Psest_2514 0.75 +0.2 2,692,607 + +2.9 2,692,607 + -1.6 2,692,657 + +1.9 2,692,657 + -0.2 2,692,657 + +0.3 2,692,657 + +0.2 2,692,658 - -0.4 2,692,840 + -0.4 2,692,869 + +0.7 2,692,869 + -1.8 2,692,869 + -1.9 2,692,870 - -2.0 2,692,896 + Psest_2515 0.12 -2.3 2,692,896 + Psest_2515 0.12 -0.7 2,692,896 + Psest_2515 0.12 +0.3 2,692,896 + Psest_2515 0.12 +2.3 2,692,896 + Psest_2515 0.12 -2.7 2,692,896 + Psest_2515 0.12 -2.4 2,692,896 + Psest_2515 0.12 -1.7 2,692,896 + Psest_2515 0.12 -1.6 2,692,896 + Psest_2515 0.12 +1.7 2,692,897 - Psest_2515 0.13 -2.5 2,692,897 - Psest_2515 0.13 +1.8 2,692,897 - Psest_2515 0.13 -3.4 2,692,897 - Psest_2515 0.13 -1.3 2,692,897 - Psest_2515 0.13 +0.0 2,692,897 - Psest_2515 0.13 -3.2 2,693,047 + Psest_2515 0.29 -2.9 2,693,107 + Psest_2515 0.36 -1.1 2,693,910 - -0.7 2,693,910 - -1.2 2,693,910 - +0.9 2,693,987 + Psest_2516 0.12 +0.3 2,694,024 + Psest_2516 0.14 -0.4 2,694,053 + Psest_2516 0.15 +0.8 2,694,059 + Psest_2516 0.15 -1.1 2,694,060 - Psest_2516 0.15 +0.2 2,694,131 + Psest_2516 0.18 -0.3 2,694,131 + Psest_2516 0.18 +0.1 2,694,131 + Psest_2516 0.18 -2.3 2,694,131 + Psest_2516 0.18 -1.1 2,694,131 + Psest_2516 0.18 -2.4 2,694,132 - Psest_2516 0.18 +1.4 2,694,132 - Psest_2516 0.18 -2.4 2,694,132 - Psest_2516 0.18 +1.4 2,694,219 + Psest_2516 0.22 +0.6 2,694,296 + Psest_2516 0.25 +3.9 2,694,297 - Psest_2516 0.25 +0.4 2,694,297 - Psest_2516 0.25 +0.4 2,694,305 + Psest_2516 0.25 +1.9 2,694,306 - Psest_2516 0.25 +2.0 2,694,306 - Psest_2516 0.25 +0.6 2,694,306 - Psest_2516 0.25 +0.6 2,694,476 + Psest_2516 0.32 +0.7 2,694,476 + Psest_2516 0.32 +1.1 2,694,476 + Psest_2516 0.32 -0.9 2,694,476 + Psest_2516 0.32 -2.1 2,694,476 + Psest_2516 0.32 -3.0 2,694,476 + Psest_2516 0.32 -3.0 2,694,476 + Psest_2516 0.32 +0.2 2,694,477 - Psest_2516 0.32 -1.5 2,694,477 - Psest_2516 0.32 -1.1 2,694,477 - Psest_2516 0.32 +0.6 2,694,477 - Psest_2516 0.32 +1.0 2,694,477 - Psest_2516 0.32 -2.4 2,694,477 - Psest_2516 0.32 +0.9 2,694,477 - Psest_2516 0.32 +0.5 2,694,477 - Psest_2516 0.32 -0.7
Or see this region's nucleotide sequence