Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0230

Experiment: RCH2 grown on a colony of N2F3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0228 and Psest_0229 are separated by 88 nucleotidesPsest_0229 and Psest_0230 are separated by 87 nucleotidesPsest_0230 and Psest_0231 overlap by 8 nucleotidesPsest_0231 and Psest_0232 are separated by 97 nucleotides Psest_0228: Psest_0228 - Glycerophosphoryl diester phosphodiesterase, at 237,466 to 238,581 _0228 Psest_0229: Psest_0229 - Dihydrofolate reductase, at 238,670 to 239,248 _0229 Psest_0230: Psest_0230 - Uncharacterized BCR, YitT family COG1284., at 239,336 to 239,983 _0230 Psest_0231: Psest_0231 - phosphoesterase, MJ0936 family, at 239,976 to 240,431 _0231 Psest_0232: Psest_0232 - Predicted dehydrogenase, at 240,529 to 241,647 _0232 Position (kb) 239 240Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 238.361 kb on + strand, within Psest_0228at 238.361 kb on + strand, within Psest_0228at 238.362 kb on - strand, within Psest_0228at 238.362 kb on - strand, within Psest_0228at 238.362 kb on - strand, within Psest_0228at 238.391 kb on + strand, within Psest_0228at 238.392 kb on - strand, within Psest_0228at 238.658 kb on + strandat 238.658 kb on + strandat 238.658 kb on + strandat 238.659 kb on - strandat 238.659 kb on - strandat 238.659 kb on - strandat 238.659 kb on - strandat 238.717 kb on + strandat 238.717 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.301 kb on + strandat 239.302 kb on - strandat 239.302 kb on - strandat 239.302 kb on - strandat 239.465 kb on + strand, within Psest_0230at 239.466 kb on - strand, within Psest_0230at 239.466 kb on - strand, within Psest_0230at 239.466 kb on - strand, within Psest_0230at 239.466 kb on - strand, within Psest_0230at 239.466 kb on - strand, within Psest_0230at 239.522 kb on + strand, within Psest_0230at 239.576 kb on + strand, within Psest_0230at 239.576 kb on + strand, within Psest_0230at 239.576 kb on + strand, within Psest_0230at 239.576 kb on + strand, within Psest_0230at 239.576 kb on + strand, within Psest_0230at 239.576 kb on + strand, within Psest_0230at 239.576 kb on + strand, within Psest_0230at 239.577 kb on - strand, within Psest_0230at 239.596 kb on + strand, within Psest_0230at 239.597 kb on - strand, within Psest_0230at 239.702 kb on + strand, within Psest_0230at 239.703 kb on - strand, within Psest_0230at 239.703 kb on - strand, within Psest_0230at 239.703 kb on - strand, within Psest_0230at 239.703 kb on - strand, within Psest_0230at 239.703 kb on - strand, within Psest_0230at 239.966 kb on + strandat 239.966 kb on + strandat 239.967 kb on - strandat 239.967 kb on - strandat 239.978 kb on - strandat 240.085 kb on + strand, within Psest_0231at 240.085 kb on + strand, within Psest_0231at 240.085 kb on + strand, within Psest_0231at 240.085 kb on + strand, within Psest_0231at 240.085 kb on + strand, within Psest_0231at 240.085 kb on + strand, within Psest_0231at 240.086 kb on - strand, within Psest_0231at 240.086 kb on - strand, within Psest_0231at 240.086 kb on - strand, within Psest_0231at 240.119 kb on + strand, within Psest_0231at 240.241 kb on - strand, within Psest_0231at 240.446 kb on + strandat 240.447 kb on - strandat 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.691 kb on + strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.692 kb on - strand, within Psest_0232at 240.923 kb on + strand, within Psest_0232at 240.923 kb on + strand, within Psest_0232at 240.923 kb on + strand, within Psest_0232at 240.923 kb on + strand, within Psest_0232at 240.923 kb on + strand, within Psest_0232at 240.923 kb on + strand, within Psest_0232at 240.923 kb on + strand, within Psest_0232at 240.924 kb on - strand, within Psest_0232at 240.924 kb on - strand, within Psest_0232at 240.924 kb on - strand, within Psest_0232at 240.924 kb on - strand, within Psest_0232at 240.924 kb on - strand, within Psest_0232at 240.924 kb on - strand, within Psest_0232at 240.924 kb on - strand, within Psest_0232

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3
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238,361 + Psest_0228 0.80 -0.0
238,361 + Psest_0228 0.80 -0.3
238,362 - Psest_0228 0.80 -0.8
238,362 - Psest_0228 0.80 +2.7
238,362 - Psest_0228 0.80 -3.6
238,391 + Psest_0228 0.83 -0.1
238,392 - Psest_0228 0.83 +1.2
238,658 + -0.5
238,658 + +0.7
238,658 + -2.7
238,659 - +0.8
238,659 - +2.1
238,659 - +2.0
238,659 - +1.9
238,717 + -1.1
238,717 + -1.5
239,301 + +3.0
239,301 + -0.7
239,301 + -3.4
239,301 + -1.5
239,301 + -1.0
239,301 + +1.1
239,301 + +0.4
239,301 + +1.7
239,301 + -2.2
239,301 + -1.6
239,302 - +1.5
239,302 - -0.5
239,302 - +0.8
239,465 + Psest_0230 0.20 +0.6
239,466 - Psest_0230 0.20 -1.5
239,466 - Psest_0230 0.20 +1.4
239,466 - Psest_0230 0.20 -1.0
239,466 - Psest_0230 0.20 -0.2
239,466 - Psest_0230 0.20 -1.6
239,522 + Psest_0230 0.29 -1.7
239,576 + Psest_0230 0.37 +0.1
239,576 + Psest_0230 0.37 +0.3
239,576 + Psest_0230 0.37 +0.5
239,576 + Psest_0230 0.37 -2.4
239,576 + Psest_0230 0.37 +0.7
239,576 + Psest_0230 0.37 +3.7
239,576 + Psest_0230 0.37 -2.9
239,577 - Psest_0230 0.37 +0.4
239,596 + Psest_0230 0.40 +2.3
239,597 - Psest_0230 0.40 +2.3
239,702 + Psest_0230 0.56 +1.6
239,703 - Psest_0230 0.57 +1.2
239,703 - Psest_0230 0.57 -2.0
239,703 - Psest_0230 0.57 -0.6
239,703 - Psest_0230 0.57 +1.5
239,703 - Psest_0230 0.57 -4.0
239,966 + +0.5
239,966 + -0.9
239,967 - -1.8
239,967 - +1.3
239,978 - -0.1
240,085 + Psest_0231 0.24 +1.2
240,085 + Psest_0231 0.24 -1.4
240,085 + Psest_0231 0.24 -1.9
240,085 + Psest_0231 0.24 +2.8
240,085 + Psest_0231 0.24 +1.6
240,085 + Psest_0231 0.24 -2.4
240,086 - Psest_0231 0.24 +3.8
240,086 - Psest_0231 0.24 -3.2
240,086 - Psest_0231 0.24 +0.7
240,119 + Psest_0231 0.31 -0.4
240,241 - Psest_0231 0.58 -0.5
240,446 + +0.7
240,447 - +1.8
240,691 + Psest_0232 0.14 +0.4
240,691 + Psest_0232 0.14 +1.2
240,691 + Psest_0232 0.14 +0.2
240,691 + Psest_0232 0.14 +0.3
240,691 + Psest_0232 0.14 +2.1
240,691 + Psest_0232 0.14 -1.7
240,691 + Psest_0232 0.14 +3.0
240,691 + Psest_0232 0.14 +2.0
240,691 + Psest_0232 0.14 -0.9
240,691 + Psest_0232 0.14 +0.0
240,691 + Psest_0232 0.14 +0.5
240,691 + Psest_0232 0.14 +1.3
240,691 + Psest_0232 0.14 +1.3
240,692 - Psest_0232 0.15 +0.8
240,692 - Psest_0232 0.15 -0.2
240,692 - Psest_0232 0.15 +2.5
240,692 - Psest_0232 0.15 -0.8
240,692 - Psest_0232 0.15 +1.7
240,692 - Psest_0232 0.15 +4.9
240,692 - Psest_0232 0.15 -2.5
240,692 - Psest_0232 0.15 -1.6
240,923 + Psest_0232 0.35 +1.3
240,923 + Psest_0232 0.35 +2.0
240,923 + Psest_0232 0.35 -0.3
240,923 + Psest_0232 0.35 -2.3
240,923 + Psest_0232 0.35 -2.0
240,923 + Psest_0232 0.35 +3.7
240,923 + Psest_0232 0.35 +1.2
240,924 - Psest_0232 0.35 -1.6
240,924 - Psest_0232 0.35 +1.4
240,924 - Psest_0232 0.35 +0.9
240,924 - Psest_0232 0.35 +2.1
240,924 - Psest_0232 0.35 +1.7
240,924 - Psest_0232 0.35 -0.3
240,924 - Psest_0232 0.35 -0.9

Or see this region's nucleotide sequence