Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0020 and Psest_0021 are separated by 74 nucleotides Psest_0021 and Psest_0022 overlap by 4 nucleotides Psest_0022 and Psest_0023 are separated by 55 nucleotides
Psest_0020: Psest_0020 - K+ transport systems, NAD-binding component, at 20,255 to 21,628
_0020
Psest_0021: Psest_0021 - ribosomal RNA small subunit methyltransferase RsmB, at 21,703 to 23,025
_0021
Psest_0022: Psest_0022 - methionyl-tRNA formyltransferase, at 23,022 to 23,966
_0022
Psest_0023: Psest_0023 - peptide deformylase, at 24,022 to 24,528
_0023
Position (kb)
21
22
23
24 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 20.765 kb on + strand, within Psest_0020 at 20.766 kb on - strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.938 kb on - strand, within Psest_0020 at 21.110 kb on + strand, within Psest_0020 at 21.150 kb on + strand, within Psest_0020 at 21.150 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.318 kb on + strand, within Psest_0020 at 21.319 kb on - strand, within Psest_0020 at 21.355 kb on + strand, within Psest_0020 at 21.705 kb on - strand at 21.752 kb on + strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.917 kb on + strand, within Psest_0021 at 21.918 kb on - strand, within Psest_0021 at 21.918 kb on - strand, within Psest_0021 at 22.147 kb on + strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.473 kb on - strand, within Psest_0021 at 22.770 kb on - strand, within Psest_0021 at 22.770 kb on - strand, within Psest_0021 at 22.770 kb on - strand, within Psest_0021 at 22.781 kb on + strand, within Psest_0021 at 22.782 kb on - strand, within Psest_0021
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 20,765 + Psest_0020 0.37 -2.0 20,766 - Psest_0020 0.37 -1.8 20,937 + Psest_0020 0.50 -0.5 20,937 + Psest_0020 0.50 +0.1 20,937 + Psest_0020 0.50 +2.1 20,937 + Psest_0020 0.50 -0.7 20,937 + Psest_0020 0.50 +2.1 20,937 + Psest_0020 0.50 -1.7 20,937 + Psest_0020 0.50 +0.1 20,938 - Psest_0020 0.50 -2.5 21,110 + Psest_0020 0.62 +1.5 21,150 + Psest_0020 0.65 +1.3 21,150 + Psest_0020 0.65 -0.7 21,237 + Psest_0020 0.71 +2.8 21,237 + Psest_0020 0.71 -1.5 21,237 + Psest_0020 0.71 +0.3 21,237 + Psest_0020 0.71 -1.1 21,318 + Psest_0020 0.77 +0.9 21,319 - Psest_0020 0.77 +0.3 21,355 + Psest_0020 0.80 -0.5 21,705 - +0.5 21,752 + +0.3 21,753 - -0.5 21,753 - +0.5 21,753 - -2.6 21,753 - -3.1 21,753 - +1.4 21,755 + -0.7 21,755 + -1.7 21,755 + -2.4 21,755 + -2.1 21,755 + -0.7 21,756 - -2.1 21,756 - -0.5 21,756 - -2.4 21,756 - -1.6 21,756 - -2.1 21,756 - -0.9 21,756 - -0.3 21,756 - +1.7 21,756 - -1.8 21,756 - -2.6 21,756 - +0.8 21,756 - -2.0 21,756 - -2.4 21,756 - +1.1 21,917 + Psest_0021 0.16 +1.2 21,918 - Psest_0021 0.16 +1.4 21,918 - Psest_0021 0.16 -2.1 22,147 + Psest_0021 0.34 -2.7 22,148 - Psest_0021 0.34 -2.3 22,148 - Psest_0021 0.34 -1.1 22,148 - Psest_0021 0.34 -0.1 22,148 - Psest_0021 0.34 -3.0 22,148 - Psest_0021 0.34 -1.1 22,148 - Psest_0021 0.34 -0.6 22,473 - Psest_0021 0.58 -2.0 22,770 - Psest_0021 0.81 +0.7 22,770 - Psest_0021 0.81 +1.1 22,770 - Psest_0021 0.81 -1.1 22,781 + Psest_0021 0.81 -1.1 22,782 - Psest_0021 0.82 -0.8
Or see this region's nucleotide sequence