Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0208 and Psest_0209 are separated by 1 nucleotides Psest_0209 and Psest_0210 overlap by 4 nucleotides
Psest_0208: Psest_0208 - Uncharacterized protein conserved in bacteria, at 207,839 to 209,353
_0208
Psest_0209: Psest_0209 - membrane protein AbrB duplication, at 209,355 to 210,383
_0209
Psest_0210: Psest_0210 - Na/Pi-cotransporter, at 210,380 to 212,065
_0210
Position (kb)
209
210
211 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 208.416 kb on - strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.679 kb on + strand, within Psest_0208 at 208.680 kb on - strand, within Psest_0208 at 208.680 kb on - strand, within Psest_0208 at 208.680 kb on - strand, within Psest_0208 at 208.680 kb on - strand, within Psest_0208 at 208.680 kb on - strand, within Psest_0208 at 208.859 kb on + strand, within Psest_0208 at 208.859 kb on + strand, within Psest_0208 at 208.860 kb on - strand, within Psest_0208 at 208.860 kb on - strand, within Psest_0208 at 208.860 kb on - strand, within Psest_0208 at 208.860 kb on - strand, within Psest_0208 at 209.054 kb on + strand, within Psest_0208 at 209.055 kb on - strand, within Psest_0208 at 209.114 kb on + strand, within Psest_0208 at 209.238 kb on - strand at 209.238 kb on - strand at 209.309 kb on + strand at 209.509 kb on + strand, within Psest_0209 at 209.899 kb on + strand, within Psest_0209 at 210.569 kb on + strand, within Psest_0210 at 210.569 kb on + strand, within Psest_0210 at 210.695 kb on + strand, within Psest_0210 at 210.695 kb on + strand, within Psest_0210 at 210.696 kb on - strand, within Psest_0210 at 210.696 kb on - strand, within Psest_0210 at 210.833 kb on + strand, within Psest_0210 at 210.833 kb on + strand, within Psest_0210 at 210.833 kb on + strand, within Psest_0210 at 210.833 kb on + strand, within Psest_0210 at 210.833 kb on + strand, within Psest_0210 at 210.833 kb on + strand, within Psest_0210 at 210.834 kb on - strand, within Psest_0210 at 210.834 kb on - strand, within Psest_0210 at 210.834 kb on - strand, within Psest_0210 at 210.834 kb on - strand, within Psest_0210 at 210.915 kb on + strand, within Psest_0210 at 210.915 kb on + strand, within Psest_0210 at 210.933 kb on + strand, within Psest_0210 at 210.933 kb on + strand, within Psest_0210 at 210.934 kb on - strand, within Psest_0210 at 210.934 kb on - strand, within Psest_0210 at 211.010 kb on + strand, within Psest_0210 at 211.036 kb on + strand, within Psest_0210 at 211.161 kb on + strand, within Psest_0210 at 211.161 kb on + strand, within Psest_0210 at 211.161 kb on + strand, within Psest_0210 at 211.161 kb on + strand, within Psest_0210 at 211.162 kb on - strand, within Psest_0210 at 211.162 kb on - strand, within Psest_0210 at 211.224 kb on + strand, within Psest_0210 at 211.224 kb on + strand, within Psest_0210 at 211.224 kb on + strand, within Psest_0210 at 211.225 kb on - strand, within Psest_0210 at 211.332 kb on + strand, within Psest_0210 at 211.332 kb on + strand, within Psest_0210 at 211.333 kb on - strand, within Psest_0210 at 211.333 kb on - strand, within Psest_0210 at 211.333 kb on - strand, within Psest_0210 at 211.333 kb on - strand, within Psest_0210 at 211.333 kb on - strand, within Psest_0210 at 211.333 kb on - strand, within Psest_0210 at 211.333 kb on - strand, within Psest_0210
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 208,416 - Psest_0208 0.38 +1.1 208,679 + Psest_0208 0.55 -3.2 208,679 + Psest_0208 0.55 +1.8 208,679 + Psest_0208 0.55 +0.2 208,679 + Psest_0208 0.55 -0.1 208,679 + Psest_0208 0.55 +1.6 208,679 + Psest_0208 0.55 +0.1 208,679 + Psest_0208 0.55 -2.3 208,679 + Psest_0208 0.55 -0.7 208,679 + Psest_0208 0.55 -0.5 208,679 + Psest_0208 0.55 +0.5 208,679 + Psest_0208 0.55 +0.8 208,680 - Psest_0208 0.56 +3.5 208,680 - Psest_0208 0.56 +0.1 208,680 - Psest_0208 0.56 +1.1 208,680 - Psest_0208 0.56 +2.2 208,680 - Psest_0208 0.56 -2.3 208,859 + Psest_0208 0.67 +3.3 208,859 + Psest_0208 0.67 -2.2 208,860 - Psest_0208 0.67 +0.8 208,860 - Psest_0208 0.67 +2.1 208,860 - Psest_0208 0.67 -2.2 208,860 - Psest_0208 0.67 +1.9 209,054 + Psest_0208 0.80 +0.4 209,055 - Psest_0208 0.80 -0.4 209,114 + Psest_0208 0.84 -2.4 209,238 - -2.9 209,238 - -2.6 209,309 + -0.4 209,509 + Psest_0209 0.15 -1.3 209,899 + Psest_0209 0.53 -1.7 210,569 + Psest_0210 0.11 +0.8 210,569 + Psest_0210 0.11 -1.6 210,695 + Psest_0210 0.19 +0.9 210,695 + Psest_0210 0.19 +0.1 210,696 - Psest_0210 0.19 -2.2 210,696 - Psest_0210 0.19 -0.5 210,833 + Psest_0210 0.27 -2.2 210,833 + Psest_0210 0.27 -2.9 210,833 + Psest_0210 0.27 +1.5 210,833 + Psest_0210 0.27 +0.5 210,833 + Psest_0210 0.27 +0.4 210,833 + Psest_0210 0.27 +0.4 210,834 - Psest_0210 0.27 -2.8 210,834 - Psest_0210 0.27 -1.8 210,834 - Psest_0210 0.27 +0.9 210,834 - Psest_0210 0.27 -1.3 210,915 + Psest_0210 0.32 -1.6 210,915 + Psest_0210 0.32 -2.7 210,933 + Psest_0210 0.33 +0.7 210,933 + Psest_0210 0.33 +3.0 210,934 - Psest_0210 0.33 +0.8 210,934 - Psest_0210 0.33 -2.1 211,010 + Psest_0210 0.37 +0.3 211,036 + Psest_0210 0.39 +2.5 211,161 + Psest_0210 0.46 +1.4 211,161 + Psest_0210 0.46 +0.6 211,161 + Psest_0210 0.46 -1.7 211,161 + Psest_0210 0.46 -3.2 211,162 - Psest_0210 0.46 +0.3 211,162 - Psest_0210 0.46 +0.3 211,224 + Psest_0210 0.50 -0.1 211,224 + Psest_0210 0.50 -1.7 211,224 + Psest_0210 0.50 +0.8 211,225 - Psest_0210 0.50 +0.0 211,332 + Psest_0210 0.56 +2.6 211,332 + Psest_0210 0.56 -1.8 211,333 - Psest_0210 0.57 +0.9 211,333 - Psest_0210 0.57 -3.5 211,333 - Psest_0210 0.57 +1.3 211,333 - Psest_0210 0.57 +1.0 211,333 - Psest_0210 0.57 -1.5 211,333 - Psest_0210 0.57 +0.7 211,333 - Psest_0210 0.57 -2.6
Or see this region's nucleotide sequence