Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2057 and Psest_2058 are separated by 47 nucleotides Psest_2058 and Psest_2059 are separated by 5 nucleotides Psest_2059 and Psest_2060 are separated by 278 nucleotides
Psest_2057: Psest_2057 - Predicted secreted protein, at 2,181,749 to 2,182,495
_2057
Psest_2058: Psest_2058 - Zn-dependent hydrolases, including glyoxylases, at 2,182,543 to 2,183,472
_2058
Psest_2059: Psest_2059 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 2,183,478 to 2,184,716
_2059
Psest_2060: Psest_2060 - PQQ-dependent dehydrogenase, methanol/ethanol family, at 2,184,995 to 2,186,773
_2060
Position (kb)
2183
2184
2185 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2182.517 kb on + strand at 2182.518 kb on - strand at 2182.575 kb on + strand at 2182.575 kb on + strand at 2182.575 kb on + strand at 2182.575 kb on + strand at 2182.576 kb on - strand at 2182.606 kb on + strand at 2182.606 kb on + strand at 2182.607 kb on - strand at 2182.607 kb on - strand at 2182.702 kb on - strand, within Psest_2058 at 2182.763 kb on - strand, within Psest_2058 at 2182.850 kb on + strand, within Psest_2058 at 2182.948 kb on + strand, within Psest_2058 at 2182.948 kb on + strand, within Psest_2058 at 2182.948 kb on + strand, within Psest_2058 at 2182.948 kb on + strand, within Psest_2058 at 2182.948 kb on + strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2182.949 kb on - strand, within Psest_2058 at 2183.284 kb on + strand, within Psest_2058 at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.572 kb on + strand at 2183.629 kb on + strand, within Psest_2059 at 2183.629 kb on + strand, within Psest_2059 at 2183.629 kb on + strand, within Psest_2059 at 2183.629 kb on + strand, within Psest_2059 at 2183.629 kb on + strand, within Psest_2059 at 2183.629 kb on + strand, within Psest_2059 at 2183.630 kb on - strand, within Psest_2059 at 2183.630 kb on - strand, within Psest_2059 at 2183.630 kb on - strand, within Psest_2059 at 2183.782 kb on + strand, within Psest_2059 at 2183.783 kb on - strand, within Psest_2059 at 2183.798 kb on + strand, within Psest_2059 at 2183.908 kb on - strand, within Psest_2059 at 2183.908 kb on - strand, within Psest_2059 at 2184.097 kb on + strand, within Psest_2059 at 2184.098 kb on - strand, within Psest_2059 at 2184.137 kb on - strand, within Psest_2059 at 2184.223 kb on + strand, within Psest_2059 at 2184.224 kb on - strand, within Psest_2059 at 2184.367 kb on + strand, within Psest_2059 at 2184.368 kb on - strand, within Psest_2059 at 2184.610 kb on + strand at 2184.610 kb on + strand at 2184.610 kb on + strand at 2184.610 kb on + strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.611 kb on - strand at 2184.629 kb on - strand at 2184.678 kb on - strand at 2184.777 kb on + strand at 2184.777 kb on + strand at 2184.777 kb on + strand at 2184.868 kb on + strand at 2184.868 kb on + strand at 2184.886 kb on - strand at 2184.967 kb on + strand at 2184.967 kb on + strand at 2184.968 kb on - strand at 2185.286 kb on + strand, within Psest_2060 at 2185.286 kb on + strand, within Psest_2060 at 2185.286 kb on + strand, within Psest_2060 at 2185.286 kb on + strand, within Psest_2060 at 2185.287 kb on - strand, within Psest_2060 at 2185.287 kb on - strand, within Psest_2060 at 2185.352 kb on + strand, within Psest_2060 at 2185.352 kb on + strand, within Psest_2060 at 2185.418 kb on + strand, within Psest_2060 at 2185.419 kb on - strand, within Psest_2060 at 2185.515 kb on - strand, within Psest_2060 at 2185.515 kb on - strand, within Psest_2060 at 2185.619 kb on + strand, within Psest_2060 at 2185.619 kb on + strand, within Psest_2060 at 2185.619 kb on + strand, within Psest_2060 at 2185.679 kb on + strand, within Psest_2060
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 2,182,517 + +1.5 2,182,518 - +4.3 2,182,575 + +1.1 2,182,575 + -0.3 2,182,575 + -1.9 2,182,575 + -0.8 2,182,576 - +1.2 2,182,606 + +0.0 2,182,606 + +4.9 2,182,607 - -0.1 2,182,607 - -2.2 2,182,702 - Psest_2058 0.17 -1.6 2,182,763 - Psest_2058 0.24 +1.0 2,182,850 + Psest_2058 0.33 +0.8 2,182,948 + Psest_2058 0.44 +1.4 2,182,948 + Psest_2058 0.44 -1.9 2,182,948 + Psest_2058 0.44 -2.7 2,182,948 + Psest_2058 0.44 +0.7 2,182,948 + Psest_2058 0.44 +1.0 2,182,949 - Psest_2058 0.44 -0.3 2,182,949 - Psest_2058 0.44 +1.7 2,182,949 - Psest_2058 0.44 +2.0 2,182,949 - Psest_2058 0.44 +0.8 2,182,949 - Psest_2058 0.44 +0.0 2,182,949 - Psest_2058 0.44 +3.7 2,182,949 - Psest_2058 0.44 +1.7 2,182,949 - Psest_2058 0.44 -0.4 2,183,284 + Psest_2058 0.80 -2.0 2,183,572 + -0.5 2,183,572 + -0.8 2,183,572 + -1.8 2,183,572 + -0.0 2,183,572 + +1.9 2,183,572 + -1.2 2,183,572 + +2.1 2,183,572 + -0.4 2,183,572 + +1.6 2,183,629 + Psest_2059 0.12 -0.8 2,183,629 + Psest_2059 0.12 -2.4 2,183,629 + Psest_2059 0.12 +1.4 2,183,629 + Psest_2059 0.12 -2.5 2,183,629 + Psest_2059 0.12 +0.4 2,183,629 + Psest_2059 0.12 +2.4 2,183,630 - Psest_2059 0.12 -1.2 2,183,630 - Psest_2059 0.12 +0.7 2,183,630 - Psest_2059 0.12 +1.0 2,183,782 + Psest_2059 0.25 -0.2 2,183,783 - Psest_2059 0.25 -0.2 2,183,798 + Psest_2059 0.26 -0.5 2,183,908 - Psest_2059 0.35 -4.3 2,183,908 - Psest_2059 0.35 +0.6 2,184,097 + Psest_2059 0.50 -1.4 2,184,098 - Psest_2059 0.50 +0.9 2,184,137 - Psest_2059 0.53 -1.2 2,184,223 + Psest_2059 0.60 +2.0 2,184,224 - Psest_2059 0.60 +2.3 2,184,367 + Psest_2059 0.72 -0.3 2,184,368 - Psest_2059 0.72 -0.7 2,184,610 + +4.3 2,184,610 + -2.2 2,184,610 + +0.6 2,184,610 + -0.2 2,184,611 - -1.9 2,184,611 - +0.6 2,184,611 - +1.3 2,184,611 - +0.2 2,184,611 - +1.5 2,184,629 - -1.7 2,184,678 - -0.2 2,184,777 + -3.0 2,184,777 + -2.1 2,184,777 + -2.1 2,184,868 + +2.1 2,184,868 + -0.9 2,184,886 - -2.7 2,184,967 + -0.0 2,184,967 + -0.2 2,184,968 - +0.6 2,185,286 + Psest_2060 0.16 +1.4 2,185,286 + Psest_2060 0.16 -0.6 2,185,286 + Psest_2060 0.16 +1.3 2,185,286 + Psest_2060 0.16 +1.4 2,185,287 - Psest_2060 0.16 +1.7 2,185,287 - Psest_2060 0.16 -0.5 2,185,352 + Psest_2060 0.20 -2.7 2,185,352 + Psest_2060 0.20 -0.7 2,185,418 + Psest_2060 0.24 -0.4 2,185,419 - Psest_2060 0.24 +1.2 2,185,515 - Psest_2060 0.29 -1.0 2,185,515 - Psest_2060 0.29 +0.7 2,185,619 + Psest_2060 0.35 -1.8 2,185,619 + Psest_2060 0.35 +2.0 2,185,619 + Psest_2060 0.35 +1.7 2,185,679 + Psest_2060 0.38 +0.5
Or see this region's nucleotide sequence