Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1928 and Psest_1930 are separated by 492 nucleotides Psest_1930 and Psest_1931 are separated by 3 nucleotides Psest_1931 and Psest_1932 are separated by 67 nucleotides
Psest_1928: Psest_1928 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 2,039,198 to 2,041,648
_1928
Psest_1930: Psest_1930 - hypothetical protein, at 2,042,141 to 2,042,449
_1930
Psest_1931: Psest_1931 - Serine/threonine protein phosphatase, at 2,042,453 to 2,044,126
_1931
Psest_1932: Psest_1932 - nitrite extrusion protein (nitrite facilitator), at 2,044,194 to 2,045,405
_1932
Position (kb)
2042
2043
2044
2045 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2041.521 kb on + strand at 2041.521 kb on + strand at 2041.560 kb on + strand at 2041.804 kb on - strand at 2041.852 kb on + strand at 2041.853 kb on - strand at 2041.967 kb on + strand at 2041.968 kb on - strand at 2041.968 kb on - strand at 2042.033 kb on - strand at 2042.781 kb on + strand, within Psest_1931 at 2042.782 kb on - strand, within Psest_1931 at 2042.784 kb on + strand, within Psest_1931 at 2042.784 kb on + strand, within Psest_1931 at 2042.784 kb on + strand, within Psest_1931 at 2042.785 kb on - strand, within Psest_1931 at 2042.805 kb on + strand, within Psest_1931 at 2043.112 kb on - strand, within Psest_1931 at 2043.112 kb on - strand, within Psest_1931 at 2043.126 kb on + strand, within Psest_1931 at 2043.126 kb on + strand, within Psest_1931 at 2043.126 kb on + strand, within Psest_1931 at 2043.126 kb on + strand, within Psest_1931 at 2043.126 kb on + strand, within Psest_1931 at 2043.127 kb on - strand, within Psest_1931 at 2043.127 kb on - strand, within Psest_1931 at 2043.127 kb on - strand, within Psest_1931 at 2043.129 kb on + strand, within Psest_1931 at 2043.129 kb on + strand, within Psest_1931 at 2043.129 kb on + strand, within Psest_1931 at 2043.129 kb on + strand, within Psest_1931 at 2043.339 kb on - strand, within Psest_1931 at 2043.339 kb on - strand, within Psest_1931 at 2043.339 kb on - strand, within Psest_1931 at 2043.547 kb on + strand, within Psest_1931 at 2043.548 kb on - strand, within Psest_1931 at 2043.603 kb on + strand, within Psest_1931 at 2043.603 kb on + strand, within Psest_1931 at 2043.604 kb on - strand, within Psest_1931 at 2043.604 kb on - strand, within Psest_1931 at 2043.604 kb on - strand, within Psest_1931 at 2043.604 kb on - strand, within Psest_1931 at 2043.636 kb on + strand, within Psest_1931 at 2043.855 kb on - strand, within Psest_1931 at 2043.865 kb on + strand, within Psest_1931 at 2043.866 kb on - strand, within Psest_1931 at 2043.924 kb on + strand, within Psest_1931 at 2044.014 kb on + strand at 2044.059 kb on - strand at 2044.108 kb on - strand at 2044.108 kb on - strand at 2044.165 kb on - strand at 2044.233 kb on - strand at 2044.561 kb on + strand, within Psest_1932 at 2044.561 kb on + strand, within Psest_1932 at 2044.562 kb on - strand, within Psest_1932 at 2044.562 kb on - strand, within Psest_1932 at 2044.562 kb on - strand, within Psest_1932 at 2044.658 kb on - strand, within Psest_1932 at 2044.753 kb on + strand, within Psest_1932 at 2044.753 kb on + strand, within Psest_1932 at 2044.753 kb on + strand, within Psest_1932 at 2044.753 kb on + strand, within Psest_1932 at 2044.801 kb on + strand, within Psest_1932 at 2044.802 kb on - strand, within Psest_1932 at 2044.802 kb on - strand, within Psest_1932 at 2045.102 kb on - strand, within Psest_1932 at 2045.118 kb on + strand, within Psest_1932
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 2,041,521 + -3.4 2,041,521 + -1.9 2,041,560 + +0.0 2,041,804 - +0.4 2,041,852 + +0.1 2,041,853 - -2.5 2,041,967 + -2.0 2,041,968 - -1.4 2,041,968 - -0.7 2,042,033 - -0.6 2,042,781 + Psest_1931 0.20 +1.6 2,042,782 - Psest_1931 0.20 -0.3 2,042,784 + Psest_1931 0.20 -0.0 2,042,784 + Psest_1931 0.20 -0.7 2,042,784 + Psest_1931 0.20 -0.3 2,042,785 - Psest_1931 0.20 +2.1 2,042,805 + Psest_1931 0.21 -2.5 2,043,112 - Psest_1931 0.39 -0.6 2,043,112 - Psest_1931 0.39 -1.8 2,043,126 + Psest_1931 0.40 -2.6 2,043,126 + Psest_1931 0.40 -0.5 2,043,126 + Psest_1931 0.40 +0.1 2,043,126 + Psest_1931 0.40 -2.3 2,043,126 + Psest_1931 0.40 -0.2 2,043,127 - Psest_1931 0.40 -2.4 2,043,127 - Psest_1931 0.40 -2.5 2,043,127 - Psest_1931 0.40 +1.3 2,043,129 + Psest_1931 0.40 -3.1 2,043,129 + Psest_1931 0.40 -0.3 2,043,129 + Psest_1931 0.40 +2.2 2,043,129 + Psest_1931 0.40 +1.4 2,043,339 - Psest_1931 0.53 +1.5 2,043,339 - Psest_1931 0.53 -1.1 2,043,339 - Psest_1931 0.53 +1.9 2,043,547 + Psest_1931 0.65 +1.5 2,043,548 - Psest_1931 0.65 -2.8 2,043,603 + Psest_1931 0.69 +0.9 2,043,603 + Psest_1931 0.69 -2.1 2,043,604 - Psest_1931 0.69 -1.5 2,043,604 - Psest_1931 0.69 -2.3 2,043,604 - Psest_1931 0.69 -0.6 2,043,604 - Psest_1931 0.69 -2.8 2,043,636 + Psest_1931 0.71 +0.7 2,043,855 - Psest_1931 0.84 -2.5 2,043,865 + Psest_1931 0.84 +2.3 2,043,866 - Psest_1931 0.84 -2.3 2,043,924 + Psest_1931 0.88 -2.9 2,044,014 + -1.5 2,044,059 - +0.2 2,044,108 - -1.2 2,044,108 - -0.5 2,044,165 - +3.1 2,044,233 - +0.1 2,044,561 + Psest_1932 0.30 +1.9 2,044,561 + Psest_1932 0.30 +0.5 2,044,562 - Psest_1932 0.30 +3.8 2,044,562 - Psest_1932 0.30 +0.5 2,044,562 - Psest_1932 0.30 +0.2 2,044,658 - Psest_1932 0.38 -1.1 2,044,753 + Psest_1932 0.46 +0.2 2,044,753 + Psest_1932 0.46 -0.4 2,044,753 + Psest_1932 0.46 +1.8 2,044,753 + Psest_1932 0.46 +3.0 2,044,801 + Psest_1932 0.50 -1.5 2,044,802 - Psest_1932 0.50 -2.3 2,044,802 - Psest_1932 0.50 +0.3 2,045,102 - Psest_1932 0.75 +2.6 2,045,118 + Psest_1932 0.76 +0.3
Or see this region's nucleotide sequence