Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1804 and Psest_1805 are separated by 259 nucleotides Psest_1805 and Psest_1806 are separated by 152 nucleotides Psest_1806 and Psest_1807 are separated by 259 nucleotides
Psest_1804: Psest_1804 - Superfamily II DNA and RNA helicases, at 1,901,451 to 1,903,121
_1804
Psest_1805: Psest_1805 - integration host factor, beta subunit, at 1,903,381 to 1,903,665
_1805
Psest_1806: Psest_1806 - Chain length determinant protein, at 1,903,818 to 1,905,098
_1806
Psest_1807: Psest_1807 - Chain length determinant protein, at 1,905,358 to 1,906,638
_1807
Position (kb)
1903
1904
1905
1906 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1903.130 kb on + strand at 1903.130 kb on + strand at 1903.131 kb on - strand at 1903.135 kb on - strand at 1903.135 kb on - strand at 1903.135 kb on - strand at 1903.151 kb on + strand at 1903.151 kb on + strand at 1903.229 kb on + strand at 1903.527 kb on + strand, within Psest_1805 at 1903.528 kb on - strand, within Psest_1805 at 1903.528 kb on - strand, within Psest_1805 at 1903.544 kb on - strand, within Psest_1805 at 1903.692 kb on + strand at 1903.693 kb on - strand at 1903.693 kb on - strand at 1903.716 kb on - strand at 1903.716 kb on - strand at 1903.716 kb on - strand at 1903.716 kb on - strand at 1903.716 kb on - strand at 1903.722 kb on + strand at 1903.723 kb on - strand at 1903.803 kb on + strand at 1903.804 kb on - strand at 1903.804 kb on - strand at 1903.804 kb on - strand at 1903.804 kb on - strand at 1903.845 kb on - strand at 1903.966 kb on - strand, within Psest_1806 at 1903.983 kb on + strand, within Psest_1806 at 1903.984 kb on - strand, within Psest_1806 at 1903.984 kb on - strand, within Psest_1806 at 1904.037 kb on + strand, within Psest_1806 at 1904.037 kb on + strand, within Psest_1806 at 1904.037 kb on + strand, within Psest_1806 at 1904.037 kb on + strand, within Psest_1806 at 1904.037 kb on + strand, within Psest_1806 at 1904.037 kb on + strand, within Psest_1806 at 1904.037 kb on + strand, within Psest_1806 at 1904.038 kb on - strand, within Psest_1806 at 1904.038 kb on - strand, within Psest_1806 at 1904.062 kb on + strand, within Psest_1806 at 1904.062 kb on + strand, within Psest_1806 at 1904.062 kb on + strand, within Psest_1806 at 1904.063 kb on - strand, within Psest_1806 at 1904.091 kb on + strand, within Psest_1806 at 1904.091 kb on + strand, within Psest_1806 at 1904.091 kb on + strand, within Psest_1806 at 1904.092 kb on - strand, within Psest_1806 at 1904.092 kb on - strand, within Psest_1806 at 1904.092 kb on - strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.316 kb on + strand, within Psest_1806 at 1904.317 kb on - strand, within Psest_1806 at 1904.317 kb on - strand, within Psest_1806 at 1904.317 kb on - strand, within Psest_1806 at 1904.317 kb on - strand, within Psest_1806 at 1904.317 kb on - strand, within Psest_1806 at 1904.317 kb on - strand, within Psest_1806 at 1904.317 kb on - strand, within Psest_1806 at 1904.421 kb on + strand, within Psest_1806 at 1904.421 kb on + strand, within Psest_1806 at 1904.421 kb on + strand, within Psest_1806 at 1904.421 kb on + strand, within Psest_1806 at 1904.594 kb on + strand, within Psest_1806 at 1904.595 kb on - strand, within Psest_1806 at 1904.662 kb on + strand, within Psest_1806 at 1904.662 kb on + strand, within Psest_1806 at 1904.797 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.848 kb on + strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.849 kb on - strand, within Psest_1806 at 1904.850 kb on + strand, within Psest_1806 at 1904.850 kb on + strand, within Psest_1806 at 1904.850 kb on + strand, within Psest_1806 at 1904.851 kb on - strand, within Psest_1806 at 1905.032 kb on - strand at 1905.047 kb on - strand at 1905.096 kb on + strand at 1905.097 kb on - strand at 1905.141 kb on + strand at 1905.141 kb on + strand at 1905.141 kb on + strand at 1905.141 kb on + strand at 1905.141 kb on + strand at 1905.141 kb on + strand at 1905.142 kb on - strand at 1905.142 kb on - strand at 1905.142 kb on - strand at 1905.142 kb on - strand at 1905.142 kb on - strand at 1905.142 kb on - strand at 1905.162 kb on + strand at 1905.196 kb on - strand at 1905.217 kb on + strand at 1905.217 kb on + strand at 1905.218 kb on - strand at 1905.467 kb on + strand at 1905.468 kb on - strand at 1905.468 kb on - strand at 1905.500 kb on - strand, within Psest_1807 at 1905.520 kb on + strand, within Psest_1807 at 1905.521 kb on - strand, within Psest_1807 at 1905.523 kb on + strand, within Psest_1807 at 1905.523 kb on + strand, within Psest_1807 at 1905.523 kb on + strand, within Psest_1807 at 1905.523 kb on + strand, within Psest_1807 at 1905.524 kb on - strand, within Psest_1807 at 1905.524 kb on - strand, within Psest_1807 at 1905.542 kb on + strand, within Psest_1807 at 1905.543 kb on - strand, within Psest_1807 at 1905.588 kb on + strand, within Psest_1807 at 1905.588 kb on + strand, within Psest_1807 at 1905.588 kb on + strand, within Psest_1807 at 1905.589 kb on - strand, within Psest_1807 at 1905.589 kb on - strand, within Psest_1807 at 1905.593 kb on - strand, within Psest_1807 at 1905.593 kb on - strand, within Psest_1807 at 1905.593 kb on - strand, within Psest_1807 at 1905.593 kb on - strand, within Psest_1807 at 1905.593 kb on - strand, within Psest_1807 at 1905.698 kb on + strand, within Psest_1807 at 1905.803 kb on - strand, within Psest_1807 at 1905.803 kb on - strand, within Psest_1807 at 1905.803 kb on - strand, within Psest_1807 at 1905.823 kb on + strand, within Psest_1807 at 1905.916 kb on - strand, within Psest_1807 at 1905.936 kb on + strand, within Psest_1807 at 1905.976 kb on + strand, within Psest_1807 at 1905.977 kb on - strand, within Psest_1807 at 1905.977 kb on - strand, within Psest_1807 at 1905.977 kb on - strand, within Psest_1807 at 1905.977 kb on - strand, within Psest_1807
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,903,130 + -2.8 1,903,130 + +1.3 1,903,131 - -0.2 1,903,135 - -1.1 1,903,135 - +0.2 1,903,135 - -0.7 1,903,151 + +0.7 1,903,151 + -2.1 1,903,229 + +3.2 1,903,527 + Psest_1805 0.51 -0.6 1,903,528 - Psest_1805 0.52 -1.1 1,903,528 - Psest_1805 0.52 -3.5 1,903,544 - Psest_1805 0.57 -0.6 1,903,692 + -0.9 1,903,693 - -0.9 1,903,693 - +0.5 1,903,716 - +0.1 1,903,716 - +3.7 1,903,716 - -0.1 1,903,716 - +0.4 1,903,716 - -0.4 1,903,722 + -1.7 1,903,723 - +2.6 1,903,803 + -1.9 1,903,804 - +0.1 1,903,804 - -0.4 1,903,804 - -3.1 1,903,804 - -2.9 1,903,845 - +1.8 1,903,966 - Psest_1806 0.12 -1.6 1,903,983 + Psest_1806 0.13 -0.2 1,903,984 - Psest_1806 0.13 -2.3 1,903,984 - Psest_1806 0.13 +2.3 1,904,037 + Psest_1806 0.17 -2.2 1,904,037 + Psest_1806 0.17 +0.4 1,904,037 + Psest_1806 0.17 -1.8 1,904,037 + Psest_1806 0.17 -3.8 1,904,037 + Psest_1806 0.17 -2.3 1,904,037 + Psest_1806 0.17 -1.5 1,904,037 + Psest_1806 0.17 +1.2 1,904,038 - Psest_1806 0.17 +0.9 1,904,038 - Psest_1806 0.17 -0.6 1,904,062 + Psest_1806 0.19 -2.6 1,904,062 + Psest_1806 0.19 -0.6 1,904,062 + Psest_1806 0.19 +0.4 1,904,063 - Psest_1806 0.19 +1.1 1,904,091 + Psest_1806 0.21 -1.1 1,904,091 + Psest_1806 0.21 +1.6 1,904,091 + Psest_1806 0.21 -0.8 1,904,092 - Psest_1806 0.21 +0.7 1,904,092 - Psest_1806 0.21 -3.5 1,904,092 - Psest_1806 0.21 -2.8 1,904,316 + Psest_1806 0.39 +0.9 1,904,316 + Psest_1806 0.39 +1.8 1,904,316 + Psest_1806 0.39 +0.8 1,904,316 + Psest_1806 0.39 +0.1 1,904,316 + Psest_1806 0.39 -0.9 1,904,316 + Psest_1806 0.39 +1.5 1,904,316 + Psest_1806 0.39 +0.1 1,904,316 + Psest_1806 0.39 -0.9 1,904,316 + Psest_1806 0.39 -0.0 1,904,316 + Psest_1806 0.39 -1.4 1,904,317 - Psest_1806 0.39 -1.7 1,904,317 - Psest_1806 0.39 -3.3 1,904,317 - Psest_1806 0.39 -0.1 1,904,317 - Psest_1806 0.39 +3.1 1,904,317 - Psest_1806 0.39 -2.1 1,904,317 - Psest_1806 0.39 -1.5 1,904,317 - Psest_1806 0.39 +1.0 1,904,421 + Psest_1806 0.47 +0.9 1,904,421 + Psest_1806 0.47 +0.5 1,904,421 + Psest_1806 0.47 -2.7 1,904,421 + Psest_1806 0.47 +0.5 1,904,594 + Psest_1806 0.61 -0.4 1,904,595 - Psest_1806 0.61 -3.4 1,904,662 + Psest_1806 0.66 +0.1 1,904,662 + Psest_1806 0.66 -0.4 1,904,797 + Psest_1806 0.76 -2.6 1,904,848 + Psest_1806 0.80 +0.0 1,904,848 + Psest_1806 0.80 -2.1 1,904,848 + Psest_1806 0.80 -2.4 1,904,848 + Psest_1806 0.80 -1.2 1,904,848 + Psest_1806 0.80 -2.9 1,904,848 + Psest_1806 0.80 +4.1 1,904,848 + Psest_1806 0.80 -2.6 1,904,848 + Psest_1806 0.80 -0.6 1,904,848 + Psest_1806 0.80 +1.2 1,904,848 + Psest_1806 0.80 -0.1 1,904,849 - Psest_1806 0.80 +0.8 1,904,849 - Psest_1806 0.80 +0.1 1,904,849 - Psest_1806 0.80 -4.0 1,904,849 - Psest_1806 0.80 -0.4 1,904,849 - Psest_1806 0.80 -1.8 1,904,849 - Psest_1806 0.80 -1.3 1,904,849 - Psest_1806 0.80 +3.0 1,904,849 - Psest_1806 0.80 -2.4 1,904,849 - Psest_1806 0.80 +1.0 1,904,849 - Psest_1806 0.80 -0.6 1,904,849 - Psest_1806 0.80 -1.9 1,904,850 + Psest_1806 0.81 -4.1 1,904,850 + Psest_1806 0.81 +0.4 1,904,850 + Psest_1806 0.81 +0.1 1,904,851 - Psest_1806 0.81 -0.7 1,905,032 - +1.5 1,905,047 - -0.9 1,905,096 + +1.1 1,905,097 - +0.7 1,905,141 + +0.8 1,905,141 + +0.0 1,905,141 + -1.4 1,905,141 + +0.8 1,905,141 + +0.4 1,905,141 + +0.3 1,905,142 - +0.3 1,905,142 - -1.5 1,905,142 - -0.1 1,905,142 - -0.9 1,905,142 - -0.5 1,905,142 - +0.9 1,905,162 + -1.4 1,905,196 - +1.4 1,905,217 + -3.7 1,905,217 + -1.5 1,905,218 - +1.5 1,905,467 + +1.9 1,905,468 - +0.7 1,905,468 - -2.6 1,905,500 - Psest_1807 0.11 -1.2 1,905,520 + Psest_1807 0.13 -1.3 1,905,521 - Psest_1807 0.13 +2.1 1,905,523 + Psest_1807 0.13 +1.3 1,905,523 + Psest_1807 0.13 +0.8 1,905,523 + Psest_1807 0.13 -2.4 1,905,523 + Psest_1807 0.13 +0.9 1,905,524 - Psest_1807 0.13 +2.2 1,905,524 - Psest_1807 0.13 +0.6 1,905,542 + Psest_1807 0.14 +0.7 1,905,543 - Psest_1807 0.14 -2.7 1,905,588 + Psest_1807 0.18 +1.5 1,905,588 + Psest_1807 0.18 +0.8 1,905,588 + Psest_1807 0.18 +1.8 1,905,589 - Psest_1807 0.18 -2.0 1,905,589 - Psest_1807 0.18 +0.4 1,905,593 - Psest_1807 0.18 -0.4 1,905,593 - Psest_1807 0.18 -0.6 1,905,593 - Psest_1807 0.18 -2.0 1,905,593 - Psest_1807 0.18 +0.2 1,905,593 - Psest_1807 0.18 -0.1 1,905,698 + Psest_1807 0.27 +1.3 1,905,803 - Psest_1807 0.35 -2.9 1,905,803 - Psest_1807 0.35 +0.3 1,905,803 - Psest_1807 0.35 -1.6 1,905,823 + Psest_1807 0.36 +1.3 1,905,916 - Psest_1807 0.44 +0.4 1,905,936 + Psest_1807 0.45 +2.1 1,905,976 + Psest_1807 0.48 -1.1 1,905,977 - Psest_1807 0.48 -0.6 1,905,977 - Psest_1807 0.48 -0.3 1,905,977 - Psest_1807 0.48 +1.1 1,905,977 - Psest_1807 0.48 +0.5
Or see this region's nucleotide sequence