Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1801 and Psest_1802 are separated by 132 nucleotides Psest_1802 and Psest_1803 are separated by 248 nucleotides
Psest_1801: Psest_1801 - 3-deoxy-7-phosphoheptulonate synthase, class II, at 1,896,424 to 1,897,773
_1801
Psest_1802: Psest_1802 - Spermidine synthase, at 1,897,906 to 1,898,655
_1802
Psest_1803: Psest_1803 - Methyl-accepting chemotaxis protein, at 1,898,904 to 1,901,066
_1803
Position (kb)
1897
1898
1899 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.055 kb on + strand, within Psest_1801 at 1897.056 kb on - strand, within Psest_1801 at 1897.056 kb on - strand, within Psest_1801 at 1897.143 kb on + strand, within Psest_1801 at 1897.178 kb on + strand, within Psest_1801 at 1897.178 kb on + strand, within Psest_1801 at 1897.855 kb on + strand at 1897.856 kb on - strand at 1897.965 kb on - strand at 1898.024 kb on + strand, within Psest_1802 at 1898.025 kb on - strand, within Psest_1802 at 1898.059 kb on + strand, within Psest_1802 at 1898.059 kb on + strand, within Psest_1802 at 1898.121 kb on - strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.257 kb on + strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.258 kb on - strand, within Psest_1802 at 1898.330 kb on - strand, within Psest_1802 at 1898.330 kb on - strand, within Psest_1802 at 1898.653 kb on + strand at 1898.654 kb on - strand at 1898.838 kb on - strand at 1898.941 kb on + strand at 1898.976 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1898.987 kb on - strand at 1899.024 kb on + strand at 1899.024 kb on + strand at 1899.024 kb on + strand at 1899.024 kb on + strand at 1899.025 kb on - strand at 1899.025 kb on - strand at 1899.025 kb on - strand at 1899.076 kb on + strand at 1899.077 kb on - strand at 1899.092 kb on + strand at 1899.402 kb on + strand, within Psest_1803 at 1899.402 kb on + strand, within Psest_1803 at 1899.402 kb on + strand, within Psest_1803 at 1899.500 kb on + strand, within Psest_1803 at 1899.500 kb on + strand, within Psest_1803 at 1899.500 kb on + strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.501 kb on - strand, within Psest_1803 at 1899.525 kb on + strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803 at 1899.526 kb on - strand, within Psest_1803
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 1,897,055 + Psest_1801 0.47 +2.4 1,897,055 + Psest_1801 0.47 +2.5 1,897,055 + Psest_1801 0.47 -0.0 1,897,055 + Psest_1801 0.47 -0.4 1,897,055 + Psest_1801 0.47 -1.6 1,897,055 + Psest_1801 0.47 -2.7 1,897,056 - Psest_1801 0.47 +2.2 1,897,056 - Psest_1801 0.47 +1.7 1,897,143 + Psest_1801 0.53 +1.0 1,897,178 + Psest_1801 0.56 +0.4 1,897,178 + Psest_1801 0.56 -2.4 1,897,855 + +0.2 1,897,856 - +0.8 1,897,965 - +0.8 1,898,024 + Psest_1802 0.16 +1.4 1,898,025 - Psest_1802 0.16 +0.5 1,898,059 + Psest_1802 0.20 -0.6 1,898,059 + Psest_1802 0.20 +1.1 1,898,121 - Psest_1802 0.29 +2.5 1,898,257 + Psest_1802 0.47 -0.8 1,898,257 + Psest_1802 0.47 +1.1 1,898,257 + Psest_1802 0.47 -0.7 1,898,257 + Psest_1802 0.47 +1.4 1,898,257 + Psest_1802 0.47 -1.9 1,898,258 - Psest_1802 0.47 +1.8 1,898,258 - Psest_1802 0.47 +0.4 1,898,258 - Psest_1802 0.47 -1.6 1,898,258 - Psest_1802 0.47 -0.2 1,898,258 - Psest_1802 0.47 -1.1 1,898,330 - Psest_1802 0.57 -1.4 1,898,330 - Psest_1802 0.57 -1.5 1,898,653 + +0.4 1,898,654 - +1.9 1,898,838 - +1.4 1,898,941 + -1.4 1,898,976 - +1.6 1,898,987 - +0.8 1,898,987 - -1.1 1,898,987 - +0.9 1,898,987 - -2.5 1,898,987 - -1.5 1,898,987 - -0.7 1,899,024 + +0.1 1,899,024 + -1.4 1,899,024 + +0.8 1,899,024 + -2.2 1,899,025 - +0.8 1,899,025 - +0.2 1,899,025 - +1.4 1,899,076 + +1.2 1,899,077 - -0.9 1,899,092 + +2.5 1,899,402 + Psest_1803 0.23 +0.7 1,899,402 + Psest_1803 0.23 -0.9 1,899,402 + Psest_1803 0.23 +1.0 1,899,500 + Psest_1803 0.28 +2.7 1,899,500 + Psest_1803 0.28 +1.9 1,899,500 + Psest_1803 0.28 -2.6 1,899,501 - Psest_1803 0.28 +2.5 1,899,501 - Psest_1803 0.28 +1.9 1,899,501 - Psest_1803 0.28 +0.4 1,899,501 - Psest_1803 0.28 +1.2 1,899,501 - Psest_1803 0.28 -0.1 1,899,501 - Psest_1803 0.28 -0.9 1,899,525 + Psest_1803 0.29 +1.2 1,899,526 - Psest_1803 0.29 -0.3 1,899,526 - Psest_1803 0.29 -0.4 1,899,526 - Psest_1803 0.29 +4.8 1,899,526 - Psest_1803 0.29 -2.1 1,899,526 - Psest_1803 0.29 +0.1 1,899,526 - Psest_1803 0.29 -0.5
Or see this region's nucleotide sequence