Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0154 and Psest_0155 are separated by 36 nucleotides Psest_0155 and Psest_0156 are separated by 98 nucleotides Psest_0156 and Psest_0157 are separated by 86 nucleotides
Psest_0154: Psest_0154 - Uncharacterized conserved protein, at 153,331 to 153,591
_0154
Psest_0155: Psest_0155 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 153,628 to 154,371
_0155
Psest_0156: Psest_0156 - imidazoleglycerol phosphate synthase, cyclase subunit, at 154,470 to 155,240
_0156
Psest_0157: Psest_0157 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 155,327 to 156,076
_0157
Position (kb) 154
155
156 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 153.499 kb on + strand, within Psest_0154 at 153.499 kb on + strand, within Psest_0154 at 153.499 kb on + strand, within Psest_0154 at 153.500 kb on - strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.502 kb on + strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.503 kb on - strand, within Psest_0154 at 153.904 kb on + strand, within Psest_0155 at 153.904 kb on + strand, within Psest_0155 at 153.904 kb on + strand, within Psest_0155 at 153.905 kb on - strand, within Psest_0155 at 153.905 kb on - strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.925 kb on + strand, within Psest_0155 at 153.926 kb on - strand, within Psest_0155 at 153.926 kb on - strand, within Psest_0155 at 153.926 kb on - strand, within Psest_0155 at 154.123 kb on + strand, within Psest_0155 at 154.306 kb on + strand at 154.306 kb on + strand at 154.306 kb on + strand at 154.307 kb on - strand at 154.307 kb on - strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.357 kb on + strand at 154.358 kb on - strand at 154.412 kb on + strand at 154.412 kb on + strand at 154.412 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.419 kb on + strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.420 kb on - strand at 154.670 kb on - strand, within Psest_0156 at 155.184 kb on + strand at 155.184 kb on + strand at 155.184 kb on + strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.185 kb on - strand at 155.208 kb on + strand at 155.209 kb on - strand at 155.266 kb on + strand at 155.267 kb on - strand at 155.267 kb on - strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.277 kb on + strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.278 kb on - strand at 155.368 kb on - strand at 155.438 kb on + strand, within Psest_0157 at 155.439 kb on - strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.540 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.576 kb on + strand, within Psest_0157 at 155.577 kb on - strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.603 kb on + strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.604 kb on - strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.744 kb on + strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.745 kb on - strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.759 kb on + strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.760 kb on - strand, within Psest_0157 at 155.951 kb on - strand, within Psest_0157 at 155.963 kb on + strand, within Psest_0157 at 155.964 kb on - strand, within Psest_0157 at 156.069 kb on - strand at 156.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 153,499 + Psest_0154 0.64 -0.2 153,499 + Psest_0154 0.64 +1.5 153,499 + Psest_0154 0.64 +1.5 153,500 - Psest_0154 0.65 +0.9 153,502 + Psest_0154 0.66 +0.6 153,502 + Psest_0154 0.66 -0.3 153,502 + Psest_0154 0.66 +0.8 153,502 + Psest_0154 0.66 +0.0 153,502 + Psest_0154 0.66 -0.8 153,502 + Psest_0154 0.66 +0.3 153,502 + Psest_0154 0.66 -0.6 153,502 + Psest_0154 0.66 +0.8 153,502 + Psest_0154 0.66 -0.5 153,502 + Psest_0154 0.66 -1.1 153,503 - Psest_0154 0.66 -1.0 153,503 - Psest_0154 0.66 +1.3 153,503 - Psest_0154 0.66 +0.0 153,503 - Psest_0154 0.66 -3.0 153,503 - Psest_0154 0.66 -0.2 153,503 - Psest_0154 0.66 +3.5 153,904 + Psest_0155 0.37 +3.2 153,904 + Psest_0155 0.37 -2.4 153,904 + Psest_0155 0.37 +2.1 153,905 - Psest_0155 0.37 +1.5 153,905 - Psest_0155 0.37 +1.8 153,925 + Psest_0155 0.40 -2.4 153,925 + Psest_0155 0.40 -0.3 153,925 + Psest_0155 0.40 -0.5 153,925 + Psest_0155 0.40 +0.1 153,925 + Psest_0155 0.40 -1.7 153,925 + Psest_0155 0.40 -1.1 153,926 - Psest_0155 0.40 +2.2 153,926 - Psest_0155 0.40 +0.5 153,926 - Psest_0155 0.40 +1.4 154,123 + Psest_0155 0.67 -0.9 154,306 + -2.4 154,306 + -2.3 154,306 + -0.1 154,307 - +8.2 154,307 - +1.1 154,357 + +0.0 154,357 + +0.8 154,357 + -0.6 154,357 + -1.7 154,357 + +2.3 154,358 - -1.8 154,412 + -0.1 154,412 + +0.7 154,412 + -2.8 154,419 + -3.6 154,419 + -0.2 154,419 + -0.8 154,419 + -0.2 154,419 + +0.8 154,420 - +0.4 154,420 - +2.1 154,420 - -2.9 154,420 - -2.7 154,420 - -2.5 154,670 - Psest_0156 0.26 -1.9 155,184 + -1.0 155,184 + +0.3 155,184 + -1.2 155,185 - -0.2 155,185 - +2.5 155,185 - +0.1 155,185 - -1.1 155,208 + -1.2 155,209 - -2.5 155,266 + -0.4 155,267 - +0.6 155,267 - +1.9 155,277 + +0.2 155,277 + -1.7 155,277 + +4.5 155,277 + -0.7 155,277 + +1.6 155,278 - -2.6 155,278 - -0.7 155,278 - +1.3 155,278 - +0.4 155,278 - -2.5 155,278 - -1.9 155,368 - -3.1 155,438 + Psest_0157 0.15 -2.1 155,439 - Psest_0157 0.15 +1.3 155,540 + Psest_0157 0.28 +2.4 155,540 + Psest_0157 0.28 -2.0 155,576 + Psest_0157 0.33 -1.5 155,576 + Psest_0157 0.33 +1.2 155,576 + Psest_0157 0.33 -2.4 155,577 - Psest_0157 0.33 +1.4 155,603 + Psest_0157 0.37 -0.9 155,603 + Psest_0157 0.37 -0.7 155,603 + Psest_0157 0.37 -0.9 155,603 + Psest_0157 0.37 +1.1 155,604 - Psest_0157 0.37 +2.0 155,604 - Psest_0157 0.37 -1.1 155,744 + Psest_0157 0.56 -0.3 155,744 + Psest_0157 0.56 -1.2 155,744 + Psest_0157 0.56 +0.8 155,744 + Psest_0157 0.56 -1.2 155,744 + Psest_0157 0.56 -2.2 155,744 + Psest_0157 0.56 +0.5 155,745 - Psest_0157 0.56 +0.2 155,745 - Psest_0157 0.56 -0.6 155,745 - Psest_0157 0.56 -2.8 155,759 + Psest_0157 0.58 +5.7 155,759 + Psest_0157 0.58 -0.7 155,760 - Psest_0157 0.58 -0.4 155,760 - Psest_0157 0.58 -0.9 155,760 - Psest_0157 0.58 +5.3 155,760 - Psest_0157 0.58 -0.3 155,951 - Psest_0157 0.83 +2.9 155,963 + Psest_0157 0.85 -1.9 155,964 - Psest_0157 0.85 -2.4 156,069 - -0.5 156,069 - +0.8
Or see this region's nucleotide sequence