Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0013 and Psest_0014 are separated by 26 nucleotides Psest_0014 and Psest_0015 are separated by 25 nucleotides Psest_0015 and Psest_0016 are separated by 146 nucleotides Psest_0016 and Psest_0017 overlap by 4 nucleotides
Psest_0013: Psest_0013 - Lauroyl/myristoyl acyltransferase, at 15,128 to 16,015
_0013
Psest_0014: Psest_0014 - PilZ domain., at 16,042 to 16,308
_0014
Psest_0015: Psest_0015 - Tetratricopeptide repeat., at 16,334 to 16,651
_0015
Psest_0016: Psest_0016 - ectoine/hydroxyectoine ABC transporter, ATP-binding protein, at 16,798 to 17,634
_0016
Psest_0017: Psest_0017 - ectoine/hydroxyectoine ABC transporter, permease protein EhuD, at 17,631 to 18,290
_0017
Position (kb)
16
17 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 15.518 kb on + strand, within Psest_0013 at 15.518 kb on + strand, within Psest_0013 at 15.519 kb on - strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.548 kb on + strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.549 kb on - strand, within Psest_0013 at 15.723 kb on + strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.724 kb on - strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.878 kb on + strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.879 kb on - strand, within Psest_0013 at 15.932 kb on + strand at 15.932 kb on + strand at 15.932 kb on + strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.933 kb on - strand at 15.944 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.956 kb on + strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 15.957 kb on - strand at 16.041 kb on + strand at 16.042 kb on - strand at 16.042 kb on - strand at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.156 kb on + strand, within Psest_0014 at 16.157 kb on - strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.181 kb on + strand, within Psest_0014 at 16.182 kb on - strand, within Psest_0014 at 16.506 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.575 kb on + strand, within Psest_0015 at 16.576 kb on - strand, within Psest_0015 at 16.698 kb on + strand at 16.699 kb on - strand at 16.733 kb on + strand at 16.734 kb on - strand at 16.734 kb on - strand at 16.734 kb on - strand at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.287 kb on + strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.288 kb on - strand, within Psest_0016 at 17.416 kb on + strand, within Psest_0016 at 17.417 kb on - strand, within Psest_0016 at 17.628 kb on + strand at 17.629 kb on - strand at 17.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 15,518 + Psest_0013 0.44 -0.9 15,518 + Psest_0013 0.44 +3.1 15,519 - Psest_0013 0.44 +1.9 15,548 + Psest_0013 0.47 -2.1 15,548 + Psest_0013 0.47 +1.1 15,549 - Psest_0013 0.47 -2.4 15,549 - Psest_0013 0.47 -0.9 15,723 + Psest_0013 0.67 -2.9 15,724 - Psest_0013 0.67 -1.2 15,724 - Psest_0013 0.67 -2.0 15,724 - Psest_0013 0.67 -0.2 15,878 + Psest_0013 0.84 +0.2 15,878 + Psest_0013 0.84 +0.1 15,878 + Psest_0013 0.84 +0.1 15,878 + Psest_0013 0.84 -1.2 15,878 + Psest_0013 0.84 -1.1 15,878 + Psest_0013 0.84 -2.7 15,878 + Psest_0013 0.84 -2.3 15,878 + Psest_0013 0.84 -1.4 15,878 + Psest_0013 0.84 -0.2 15,878 + Psest_0013 0.84 -1.2 15,878 + Psest_0013 0.84 -0.5 15,879 - Psest_0013 0.85 -2.4 15,879 - Psest_0013 0.85 +1.1 15,879 - Psest_0013 0.85 -1.2 15,879 - Psest_0013 0.85 -1.1 15,879 - Psest_0013 0.85 -0.5 15,879 - Psest_0013 0.85 -0.4 15,879 - Psest_0013 0.85 +1.1 15,879 - Psest_0013 0.85 -1.1 15,932 + -2.8 15,932 + -0.5 15,932 + -0.6 15,933 - +1.1 15,933 - -1.7 15,933 - -0.1 15,933 - -0.7 15,944 + +2.1 15,956 + -1.4 15,956 + -1.2 15,956 + -1.5 15,956 + -1.7 15,956 + +0.1 15,956 + +2.1 15,956 + +2.4 15,956 + -0.4 15,956 + -2.5 15,956 + -1.1 15,956 + +1.2 15,956 + +5.1 15,956 + -2.0 15,956 + +0.3 15,956 + -0.2 15,956 + +0.5 15,956 + +0.1 15,957 - -0.5 15,957 - +3.6 15,957 - +1.1 15,957 - +2.1 15,957 - -1.9 15,957 - +1.1 15,957 - +2.1 16,041 + -2.1 16,042 - +2.1 16,042 - +0.7 16,156 + Psest_0014 0.43 +0.5 16,156 + Psest_0014 0.43 -3.0 16,156 + Psest_0014 0.43 +1.4 16,156 + Psest_0014 0.43 +0.2 16,157 - Psest_0014 0.43 -0.1 16,181 + Psest_0014 0.52 -1.6 16,181 + Psest_0014 0.52 -1.4 16,182 - Psest_0014 0.52 +2.5 16,506 + Psest_0015 0.54 -0.2 16,575 + Psest_0015 0.76 -0.5 16,575 + Psest_0015 0.76 +0.6 16,575 + Psest_0015 0.76 -2.6 16,576 - Psest_0015 0.76 -3.1 16,698 + +0.6 16,699 - +1.7 16,733 + -1.1 16,734 - +0.7 16,734 - +1.4 16,734 - +0.1 17,287 + Psest_0016 0.58 -0.6 17,287 + Psest_0016 0.58 +1.9 17,287 + Psest_0016 0.58 +2.0 17,287 + Psest_0016 0.58 -3.2 17,287 + Psest_0016 0.58 -1.2 17,287 + Psest_0016 0.58 -1.5 17,287 + Psest_0016 0.58 -0.1 17,288 - Psest_0016 0.59 -3.2 17,288 - Psest_0016 0.59 -0.2 17,288 - Psest_0016 0.59 +0.3 17,416 + Psest_0016 0.74 -2.1 17,417 - Psest_0016 0.74 -0.5 17,628 + +0.9 17,629 - -3.7 17,629 - -1.2
Or see this region's nucleotide sequence