Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1353

Experiment: RCH2 grown on a colony of N2F3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1352 and Psest_1353 are separated by 144 nucleotidesPsest_1353 and Psest_1354 are separated by 16 nucleotidesPsest_1354 and Psest_1355 are separated by 119 nucleotides Psest_1352: Psest_1352 - Predicted membrane protein, at 1,463,759 to 1,464,922 _1352 Psest_1353: Psest_1353 - Uncharacterized protein conserved in bacteria, at 1,465,067 to 1,465,486 _1353 Psest_1354: Psest_1354 - Uncharacterized protein conserved in bacteria, at 1,465,503 to 1,465,922 _1354 Psest_1355: Psest_1355 - Predicted protease of the Abi (CAAX) family, at 1,466,042 to 1,466,791 _1355 Position (kb) 1465 1466Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1464.099 kb on + strand, within Psest_1352at 1464.171 kb on + strand, within Psest_1352at 1464.171 kb on + strand, within Psest_1352at 1464.172 kb on - strand, within Psest_1352at 1464.172 kb on - strand, within Psest_1352at 1464.172 kb on - strand, within Psest_1352at 1464.172 kb on - strand, within Psest_1352at 1464.288 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.552 kb on + strand, within Psest_1352at 1464.553 kb on - strand, within Psest_1352at 1464.553 kb on - strand, within Psest_1352at 1464.553 kb on - strand, within Psest_1352at 1464.553 kb on - strand, within Psest_1352at 1464.553 kb on - strand, within Psest_1352at 1464.553 kb on - strand, within Psest_1352at 1464.802 kb on + strand, within Psest_1352at 1464.803 kb on - strand, within Psest_1352at 1464.993 kb on + strandat 1465.288 kb on + strand, within Psest_1353at 1465.350 kb on + strand, within Psest_1353at 1465.426 kb on + strand, within Psest_1353at 1465.426 kb on + strand, within Psest_1353at 1465.426 kb on + strand, within Psest_1353at 1465.426 kb on + strand, within Psest_1353at 1465.426 kb on + strand, within Psest_1353at 1465.426 kb on + strand, within Psest_1353at 1465.427 kb on - strand, within Psest_1353at 1465.428 kb on + strand, within Psest_1353at 1465.428 kb on + strand, within Psest_1353at 1465.428 kb on + strand, within Psest_1353at 1465.429 kb on - strand, within Psest_1353at 1465.429 kb on - strand, within Psest_1353at 1465.429 kb on - strand, within Psest_1353at 1465.429 kb on - strand, within Psest_1353at 1465.435 kb on + strand, within Psest_1353at 1465.470 kb on + strandat 1465.470 kb on + strandat 1465.470 kb on + strandat 1465.470 kb on + strandat 1465.470 kb on + strandat 1465.470 kb on + strandat 1465.470 kb on + strandat 1465.471 kb on - strandat 1465.471 kb on - strandat 1465.471 kb on - strandat 1465.806 kb on + strand, within Psest_1354at 1465.807 kb on - strand, within Psest_1354at 1465.956 kb on - strandat 1465.966 kb on - strandat 1466.041 kb on - strandat 1466.055 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3
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1,464,099 + Psest_1352 0.29 -1.9
1,464,171 + Psest_1352 0.35 +3.2
1,464,171 + Psest_1352 0.35 +0.4
1,464,172 - Psest_1352 0.35 +0.2
1,464,172 - Psest_1352 0.35 -0.5
1,464,172 - Psest_1352 0.35 -1.0
1,464,172 - Psest_1352 0.35 -0.3
1,464,288 + Psest_1352 0.45 +1.5
1,464,552 + Psest_1352 0.68 +2.2
1,464,552 + Psest_1352 0.68 -1.8
1,464,552 + Psest_1352 0.68 +2.0
1,464,552 + Psest_1352 0.68 -3.6
1,464,552 + Psest_1352 0.68 +0.9
1,464,552 + Psest_1352 0.68 -1.4
1,464,552 + Psest_1352 0.68 +1.4
1,464,552 + Psest_1352 0.68 -0.0
1,464,552 + Psest_1352 0.68 -0.5
1,464,553 - Psest_1352 0.68 -1.0
1,464,553 - Psest_1352 0.68 -0.1
1,464,553 - Psest_1352 0.68 +0.6
1,464,553 - Psest_1352 0.68 -1.7
1,464,553 - Psest_1352 0.68 +1.7
1,464,553 - Psest_1352 0.68 -2.0
1,464,802 + Psest_1352 0.90 +1.3
1,464,803 - Psest_1352 0.90 -1.6
1,464,993 + +0.9
1,465,288 + Psest_1353 0.53 -0.1
1,465,350 + Psest_1353 0.67 +1.6
1,465,426 + Psest_1353 0.85 -2.6
1,465,426 + Psest_1353 0.85 -1.0
1,465,426 + Psest_1353 0.85 -2.9
1,465,426 + Psest_1353 0.85 -1.4
1,465,426 + Psest_1353 0.85 +1.5
1,465,426 + Psest_1353 0.85 +0.2
1,465,427 - Psest_1353 0.86 -0.2
1,465,428 + Psest_1353 0.86 -2.6
1,465,428 + Psest_1353 0.86 -1.6
1,465,428 + Psest_1353 0.86 -2.1
1,465,429 - Psest_1353 0.86 -3.0
1,465,429 - Psest_1353 0.86 +0.9
1,465,429 - Psest_1353 0.86 +0.1
1,465,429 - Psest_1353 0.86 -1.3
1,465,435 + Psest_1353 0.88 -1.7
1,465,470 + +3.7
1,465,470 + +0.8
1,465,470 + -0.7
1,465,470 + +0.6
1,465,470 + +0.3
1,465,470 + +1.0
1,465,470 + +1.1
1,465,471 - -1.6
1,465,471 - +0.4
1,465,471 - -1.3
1,465,806 + Psest_1354 0.72 +1.1
1,465,807 - Psest_1354 0.72 -3.1
1,465,956 - -3.1
1,465,966 - +0.4
1,466,041 - -0.2
1,466,055 + -2.3

Or see this region's nucleotide sequence