Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0844 and Psest_0845 are separated by 16 nucleotides Psest_0845 and Psest_0846 overlap by 1 nucleotides Psest_0846 and Psest_0847 are separated by 270 nucleotides
Psest_0844: Psest_0844 - High-affinity Fe2+/Pb2+ permease, at 914,601 to 915,458
_0844
Psest_0845: Psest_0845 - hypothetical protein, at 915,475 to 915,813
_0845
Psest_0846: Psest_0846 - Uncharacterized protein probably involved in high-affinity Fe2+ transport, at 915,813 to 916,346
_0846
Psest_0847: Psest_0847 - drug resistance transporter, EmrB/QacA subfamily, at 916,617 to 918,044
_0847
Position (kb)
915
916
917 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 914.817 kb on + strand, within Psest_0844 at 914.818 kb on - strand, within Psest_0844 at 915.046 kb on + strand, within Psest_0844 at 915.047 kb on - strand, within Psest_0844 at 915.223 kb on - strand, within Psest_0844 at 915.288 kb on + strand, within Psest_0844 at 915.473 kb on + strand at 915.474 kb on - strand at 915.474 kb on - strand at 915.476 kb on + strand at 915.476 kb on + strand at 915.476 kb on + strand at 915.476 kb on + strand at 915.477 kb on - strand at 915.477 kb on - strand at 915.477 kb on - strand at 915.729 kb on - strand, within Psest_0845 at 915.857 kb on - strand at 915.955 kb on + strand, within Psest_0846 at 915.955 kb on + strand, within Psest_0846 at 915.956 kb on - strand, within Psest_0846 at 915.956 kb on - strand, within Psest_0846 at 915.967 kb on + strand, within Psest_0846 at 916.081 kb on + strand, within Psest_0846 at 916.082 kb on - strand, within Psest_0846 at 916.082 kb on - strand, within Psest_0846 at 916.455 kb on + strand at 916.475 kb on + strand at 916.475 kb on + strand at 916.584 kb on + strand at 916.636 kb on + strand at 916.636 kb on + strand at 916.637 kb on - strand at 916.637 kb on - strand at 916.788 kb on + strand, within Psest_0847 at 916.788 kb on + strand, within Psest_0847 at 916.788 kb on + strand, within Psest_0847 at 916.789 kb on - strand, within Psest_0847 at 916.789 kb on - strand, within Psest_0847 at 916.789 kb on - strand, within Psest_0847 at 916.789 kb on - strand, within Psest_0847 at 916.789 kb on - strand, within Psest_0847 at 916.789 kb on - strand, within Psest_0847 at 916.789 kb on - strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.856 kb on + strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 916.857 kb on - strand, within Psest_0847 at 917.106 kb on + strand, within Psest_0847 at 917.107 kb on - strand, within Psest_0847 at 917.107 kb on - strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.294 kb on + strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.295 kb on - strand, within Psest_0847 at 917.312 kb on - strand, within Psest_0847
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 914,817 + Psest_0844 0.25 -0.4 914,818 - Psest_0844 0.25 -0.5 915,046 + Psest_0844 0.52 -1.7 915,047 - Psest_0844 0.52 -3.1 915,223 - Psest_0844 0.72 +1.1 915,288 + Psest_0844 0.80 -0.0 915,473 + -0.1 915,474 - -2.6 915,474 - +0.7 915,476 + +1.9 915,476 + -2.7 915,476 + -2.7 915,476 + +0.9 915,477 - +2.4 915,477 - +1.3 915,477 - -1.6 915,729 - Psest_0845 0.75 -1.5 915,857 - +3.2 915,955 + Psest_0846 0.27 -0.5 915,955 + Psest_0846 0.27 -0.2 915,956 - Psest_0846 0.27 -2.3 915,956 - Psest_0846 0.27 -1.6 915,967 + Psest_0846 0.29 +3.5 916,081 + Psest_0846 0.50 +2.6 916,082 - Psest_0846 0.50 +5.8 916,082 - Psest_0846 0.50 +3.5 916,455 + +2.7 916,475 + -3.0 916,475 + -0.4 916,584 + +2.2 916,636 + -0.6 916,636 + +1.3 916,637 - +0.6 916,637 - -1.7 916,788 + Psest_0847 0.12 +0.8 916,788 + Psest_0847 0.12 -0.4 916,788 + Psest_0847 0.12 -0.9 916,789 - Psest_0847 0.12 +1.4 916,789 - Psest_0847 0.12 -1.8 916,789 - Psest_0847 0.12 +1.3 916,789 - Psest_0847 0.12 +0.6 916,789 - Psest_0847 0.12 +0.1 916,789 - Psest_0847 0.12 -1.7 916,789 - Psest_0847 0.12 +0.5 916,856 + Psest_0847 0.17 -1.3 916,856 + Psest_0847 0.17 -0.2 916,856 + Psest_0847 0.17 -0.2 916,856 + Psest_0847 0.17 +1.8 916,856 + Psest_0847 0.17 -2.1 916,856 + Psest_0847 0.17 +0.5 916,856 + Psest_0847 0.17 +0.2 916,856 + Psest_0847 0.17 -0.4 916,856 + Psest_0847 0.17 -0.7 916,857 - Psest_0847 0.17 -2.8 916,857 - Psest_0847 0.17 +1.2 916,857 - Psest_0847 0.17 +0.1 916,857 - Psest_0847 0.17 +0.2 916,857 - Psest_0847 0.17 -1.4 916,857 - Psest_0847 0.17 -1.8 916,857 - Psest_0847 0.17 -3.2 916,857 - Psest_0847 0.17 +0.4 916,857 - Psest_0847 0.17 +1.4 917,106 + Psest_0847 0.34 +1.4 917,107 - Psest_0847 0.34 +0.1 917,107 - Psest_0847 0.34 +1.4 917,294 + Psest_0847 0.47 -0.4 917,294 + Psest_0847 0.47 -2.0 917,294 + Psest_0847 0.47 -2.7 917,294 + Psest_0847 0.47 -1.3 917,294 + Psest_0847 0.47 +1.5 917,294 + Psest_0847 0.47 -1.0 917,294 + Psest_0847 0.47 -0.1 917,294 + Psest_0847 0.47 -2.1 917,294 + Psest_0847 0.47 -2.4 917,294 + Psest_0847 0.47 -0.8 917,294 + Psest_0847 0.47 -1.7 917,294 + Psest_0847 0.47 -1.9 917,294 + Psest_0847 0.47 +0.3 917,294 + Psest_0847 0.47 +1.4 917,294 + Psest_0847 0.47 +0.1 917,294 + Psest_0847 0.47 +4.8 917,294 + Psest_0847 0.47 +4.4 917,294 + Psest_0847 0.47 +0.6 917,294 + Psest_0847 0.47 +2.4 917,294 + Psest_0847 0.47 -0.9 917,294 + Psest_0847 0.47 -0.8 917,294 + Psest_0847 0.47 -0.2 917,294 + Psest_0847 0.47 -0.1 917,294 + Psest_0847 0.47 -0.0 917,294 + Psest_0847 0.47 +1.6 917,294 + Psest_0847 0.47 -1.4 917,294 + Psest_0847 0.47 -1.8 917,294 + Psest_0847 0.47 -1.3 917,294 + Psest_0847 0.47 +0.5 917,294 + Psest_0847 0.47 -0.6 917,295 - Psest_0847 0.47 -0.9 917,295 - Psest_0847 0.47 -3.1 917,295 - Psest_0847 0.47 +3.8 917,295 - Psest_0847 0.47 +0.2 917,295 - Psest_0847 0.47 +1.1 917,295 - Psest_0847 0.47 -2.4 917,295 - Psest_0847 0.47 -1.2 917,295 - Psest_0847 0.47 +1.1 917,295 - Psest_0847 0.47 -3.8 917,295 - Psest_0847 0.47 -0.4 917,295 - Psest_0847 0.47 -0.1 917,312 - Psest_0847 0.49 +3.2
Or see this region's nucleotide sequence