Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0414 and Psest_0415 are separated by 1 nucleotides Psest_0415 and Psest_0416 are separated by 85 nucleotides Psest_0416 and Psest_0417 are separated by 360 nucleotides
Psest_0414: Psest_0414 - biotin biosynthesis protein BioC, at 449,651 to 450,445
_0414
Psest_0415: Psest_0415 - dethiobiotin synthase, at 450,447 to 451,133
_0415
Psest_0416: Psest_0416 - hypothetical protein, at 451,219 to 451,467
_0416
Psest_0417: Psest_0417 - Acyl-CoA dehydrogenases, at 451,828 to 453,633
_0417
Position (kb) 451
452 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.420 kb on - strand at 450.420 kb on - strand at 450.557 kb on - strand, within Psest_0415 at 450.794 kb on - strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.838 kb on - strand, within Psest_0415 at 450.922 kb on + strand, within Psest_0415 at 450.922 kb on + strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 451.233 kb on + strand at 451.261 kb on + strand, within Psest_0416 at 451.261 kb on + strand, within Psest_0416 at 451.261 kb on + strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.633 kb on - strand at 451.633 kb on - strand at 451.633 kb on - strand at 451.633 kb on - strand at 451.671 kb on + strand at 451.671 kb on + strand at 451.672 kb on - strand at 451.672 kb on - strand at 451.713 kb on + strand at 451.713 kb on + strand at 451.713 kb on + strand at 451.809 kb on + strand at 451.822 kb on + strand at 451.837 kb on + strand at 451.837 kb on + strand at 451.838 kb on - strand at 451.868 kb on + strand at 451.868 kb on + strand at 451.869 kb on - strand at 451.889 kb on - strand at 451.901 kb on - strand at 452.275 kb on + strand, within Psest_0417 at 452.275 kb on + strand, within Psest_0417 at 452.392 kb on + strand, within Psest_0417 at 452.393 kb on - strand, within Psest_0417 at 452.393 kb on - strand, within Psest_0417
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 450,407 + -1.0 450,407 + -0.8 450,407 + -1.4 450,407 + -2.5 450,408 - -1.7 450,408 - +4.2 450,408 - -3.3 450,408 - +2.0 450,420 - -1.2 450,420 - +2.6 450,557 - Psest_0415 0.16 +0.0 450,794 - Psest_0415 0.51 -2.5 450,837 + Psest_0415 0.57 -1.8 450,837 + Psest_0415 0.57 -3.5 450,837 + Psest_0415 0.57 -0.2 450,838 - Psest_0415 0.57 -3.4 450,922 + Psest_0415 0.69 +0.7 450,922 + Psest_0415 0.69 -1.5 450,923 - Psest_0415 0.69 -1.5 450,923 - Psest_0415 0.69 +0.4 450,923 - Psest_0415 0.69 +0.6 450,923 - Psest_0415 0.69 -0.5 451,233 + +1.3 451,261 + Psest_0416 0.17 -1.1 451,261 + Psest_0416 0.17 +2.8 451,261 + Psest_0416 0.17 +3.2 451,262 - Psest_0416 0.17 -1.5 451,262 - Psest_0416 0.17 +0.4 451,262 - Psest_0416 0.17 +1.0 451,262 - Psest_0416 0.17 -0.8 451,262 - Psest_0416 0.17 -0.0 451,262 - Psest_0416 0.17 +5.1 451,262 - Psest_0416 0.17 +1.8 451,633 - +0.1 451,633 - +2.5 451,633 - +0.9 451,633 - +0.7 451,671 + +0.6 451,671 + -2.8 451,672 - -0.2 451,672 - +0.2 451,713 + +0.0 451,713 + -0.4 451,713 + +0.9 451,809 + -0.7 451,822 + +0.4 451,837 + -2.5 451,837 + -0.8 451,838 - +2.0 451,868 + -1.0 451,868 + +3.5 451,869 - -1.0 451,889 - +0.4 451,901 - +1.0 452,275 + Psest_0417 0.25 +0.4 452,275 + Psest_0417 0.25 +3.0 452,392 + Psest_0417 0.31 +1.0 452,393 - Psest_0417 0.31 -0.3 452,393 - Psest_0417 0.31 +2.0
Or see this region's nucleotide sequence