Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3650 and Psest_3651 are separated by 179 nucleotides Psest_3651 and Psest_3652 overlap by 4 nucleotides Psest_3652 and Psest_3653 are separated by 104 nucleotides
Psest_3650: Psest_3650 - anthranilate synthase component I, non-proteobacterial lineages, at 3,809,969 to 3,811,453
_3650
Psest_3651: Psest_3651 - 2-phosphoglycolate phosphatase, prokaryotic, at 3,811,633 to 3,812,334
_3651
Psest_3652: Psest_3652 - ribulose-phosphate 3-epimerase, at 3,812,331 to 3,813,005
_3652
Psest_3653: Psest_3653 - 4-aminobutyrate aminotransferase, prokaryotic type, at 3,813,110 to 3,814,390
_3653
Position (kb)
3811
3812
3813 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3810.633 kb on + strand, within Psest_3650 at 3810.633 kb on + strand, within Psest_3650 at 3810.633 kb on + strand, within Psest_3650 at 3810.634 kb on - strand, within Psest_3650 at 3810.634 kb on - strand, within Psest_3650 at 3810.634 kb on - strand, within Psest_3650 at 3810.634 kb on - strand, within Psest_3650 at 3810.634 kb on - strand, within Psest_3650 at 3810.747 kb on + strand, within Psest_3650 at 3810.747 kb on + strand, within Psest_3650 at 3810.747 kb on + strand, within Psest_3650 at 3810.747 kb on + strand, within Psest_3650 at 3810.747 kb on + strand, within Psest_3650 at 3810.747 kb on + strand, within Psest_3650 at 3810.747 kb on + strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.748 kb on - strand, within Psest_3650 at 3810.913 kb on - strand, within Psest_3650 at 3811.089 kb on + strand, within Psest_3650 at 3811.323 kb on + strand at 3811.324 kb on - strand at 3811.354 kb on - strand at 3811.354 kb on - strand at 3811.354 kb on - strand at 3811.355 kb on + strand at 3811.731 kb on - strand, within Psest_3651 at 3811.735 kb on - strand, within Psest_3651 at 3811.735 kb on - strand, within Psest_3651 at 3811.881 kb on + strand, within Psest_3651 at 3811.882 kb on - strand, within Psest_3651 at 3812.143 kb on + strand, within Psest_3651 at 3812.143 kb on + strand, within Psest_3651 at 3812.144 kb on - strand, within Psest_3651 at 3812.144 kb on - strand, within Psest_3651 at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.207 kb on + strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.208 kb on - strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.210 kb on + strand at 3813.211 kb on - strand at 3813.211 kb on - strand at 3813.211 kb on - strand at 3813.211 kb on - strand at 3813.211 kb on - strand at 3813.211 kb on - strand at 3813.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 3,810,633 + Psest_3650 0.45 -1.6 3,810,633 + Psest_3650 0.45 -1.0 3,810,633 + Psest_3650 0.45 -1.6 3,810,634 - Psest_3650 0.45 +0.7 3,810,634 - Psest_3650 0.45 -0.4 3,810,634 - Psest_3650 0.45 -2.6 3,810,634 - Psest_3650 0.45 +2.1 3,810,634 - Psest_3650 0.45 +0.1 3,810,747 + Psest_3650 0.52 -3.6 3,810,747 + Psest_3650 0.52 -1.1 3,810,747 + Psest_3650 0.52 -1.1 3,810,747 + Psest_3650 0.52 -3.1 3,810,747 + Psest_3650 0.52 -4.3 3,810,747 + Psest_3650 0.52 +0.9 3,810,747 + Psest_3650 0.52 +0.6 3,810,748 - Psest_3650 0.52 -1.0 3,810,748 - Psest_3650 0.52 +0.4 3,810,748 - Psest_3650 0.52 -1.9 3,810,748 - Psest_3650 0.52 +0.8 3,810,748 - Psest_3650 0.52 -0.1 3,810,748 - Psest_3650 0.52 -0.4 3,810,748 - Psest_3650 0.52 +1.1 3,810,748 - Psest_3650 0.52 +1.8 3,810,748 - Psest_3650 0.52 +0.5 3,810,748 - Psest_3650 0.52 -0.5 3,810,913 - Psest_3650 0.64 -1.2 3,811,089 + Psest_3650 0.75 -1.4 3,811,323 + -2.5 3,811,324 - +1.3 3,811,354 - +0.1 3,811,354 - -0.5 3,811,354 - +0.6 3,811,355 + -2.7 3,811,731 - Psest_3651 0.14 -1.6 3,811,735 - Psest_3651 0.15 -1.4 3,811,735 - Psest_3651 0.15 -0.9 3,811,881 + Psest_3651 0.35 -0.8 3,811,882 - Psest_3651 0.35 -0.2 3,812,143 + Psest_3651 0.73 -2.5 3,812,143 + Psest_3651 0.73 +0.7 3,812,144 - Psest_3651 0.73 -0.6 3,812,144 - Psest_3651 0.73 -0.4 3,813,207 + +0.5 3,813,207 + -3.9 3,813,207 + +0.1 3,813,207 + -0.0 3,813,207 + -1.7 3,813,207 + +1.4 3,813,207 + -0.6 3,813,207 + -1.7 3,813,207 + -1.6 3,813,207 + -0.0 3,813,208 - -0.4 3,813,208 - -1.6 3,813,208 - -0.2 3,813,208 - +1.0 3,813,208 - -2.1 3,813,208 - -0.0 3,813,208 - -2.7 3,813,208 - -2.9 3,813,208 - +1.0 3,813,210 + -1.9 3,813,210 + -1.5 3,813,210 + -3.3 3,813,210 + +0.0 3,813,210 + -0.0 3,813,210 + -1.0 3,813,210 + +0.4 3,813,210 + -0.1 3,813,210 + -0.9 3,813,211 - -1.3 3,813,211 - -2.3 3,813,211 - +1.4 3,813,211 - +1.0 3,813,211 - +1.7 3,813,211 - -1.6 3,813,211 - -0.1
Or see this region's nucleotide sequence