Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3536 and Psest_3537 are separated by 119 nucleotides Psest_3537 and Psest_3538 overlap by 16 nucleotides Psest_3538 and Psest_3539 are separated by 10 nucleotides
Psest_3536: Psest_3536 - hypothetical protein, at 3,714,158 to 3,714,256
_3536
Psest_3537: Psest_3537 - hypothetical protein, at 3,714,376 to 3,715,248
_3537
Psest_3538: Psest_3538 - hypothetical protein, at 3,715,233 to 3,716,282
_3538
Psest_3539: Psest_3539 - exonuclease SbcD, at 3,716,293 to 3,717,528
_3539
Position (kb)
3715
3716
3717 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3714.303 kb on + strand at 3714.306 kb on - strand at 3714.310 kb on - strand at 3714.349 kb on + strand at 3714.349 kb on + strand at 3714.349 kb on + strand at 3714.535 kb on + strand, within Psest_3537 at 3714.568 kb on + strand, within Psest_3537 at 3714.568 kb on + strand, within Psest_3537 at 3714.568 kb on + strand, within Psest_3537 at 3714.568 kb on + strand, within Psest_3537 at 3714.568 kb on + strand, within Psest_3537 at 3714.568 kb on + strand, within Psest_3537 at 3714.568 kb on + strand, within Psest_3537 at 3714.569 kb on - strand, within Psest_3537 at 3714.569 kb on - strand, within Psest_3537 at 3714.722 kb on + strand, within Psest_3537 at 3714.758 kb on + strand, within Psest_3537 at 3714.758 kb on + strand, within Psest_3537 at 3714.759 kb on - strand, within Psest_3537 at 3714.811 kb on + strand, within Psest_3537 at 3715.107 kb on - strand, within Psest_3537 at 3715.138 kb on + strand, within Psest_3537 at 3715.138 kb on + strand, within Psest_3537 at 3715.138 kb on + strand, within Psest_3537 at 3715.139 kb on - strand, within Psest_3537 at 3715.240 kb on + strand at 3715.240 kb on + strand at 3715.240 kb on + strand at 3715.240 kb on + strand at 3715.241 kb on - strand at 3715.241 kb on - strand at 3715.299 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.328 kb on + strand at 3715.329 kb on - strand at 3715.329 kb on - strand at 3715.385 kb on + strand, within Psest_3538 at 3715.470 kb on - strand, within Psest_3538 at 3715.470 kb on - strand, within Psest_3538 at 3715.470 kb on - strand, within Psest_3538 at 3715.756 kb on + strand, within Psest_3538 at 3715.757 kb on - strand, within Psest_3538 at 3715.757 kb on - strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.780 kb on + strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.781 kb on - strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.868 kb on + strand, within Psest_3538 at 3715.869 kb on - strand, within Psest_3538 at 3716.130 kb on - strand, within Psest_3538 at 3716.179 kb on + strand at 3716.179 kb on + strand at 3716.180 kb on - strand at 3716.180 kb on - strand at 3716.180 kb on - strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.215 kb on + strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.216 kb on - strand at 3716.620 kb on - strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.764 kb on + strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.765 kb on - strand, within Psest_3539 at 3716.959 kb on + strand, within Psest_3539 at 3716.960 kb on - strand, within Psest_3539 at 3716.960 kb on - strand, within Psest_3539 at 3716.960 kb on - strand, within Psest_3539 at 3717.245 kb on + strand, within Psest_3539 at 3717.246 kb on - strand, within Psest_3539
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 3,714,303 + -0.1 3,714,306 - -0.9 3,714,310 - -0.2 3,714,349 + -0.9 3,714,349 + +0.2 3,714,349 + +0.2 3,714,535 + Psest_3537 0.18 -1.9 3,714,568 + Psest_3537 0.22 +1.0 3,714,568 + Psest_3537 0.22 -1.5 3,714,568 + Psest_3537 0.22 -3.2 3,714,568 + Psest_3537 0.22 -0.4 3,714,568 + Psest_3537 0.22 +0.9 3,714,568 + Psest_3537 0.22 +1.8 3,714,568 + Psest_3537 0.22 +2.5 3,714,569 - Psest_3537 0.22 +0.3 3,714,569 - Psest_3537 0.22 -3.1 3,714,722 + Psest_3537 0.40 +1.6 3,714,758 + Psest_3537 0.44 -1.7 3,714,758 + Psest_3537 0.44 +3.2 3,714,759 - Psest_3537 0.44 +1.8 3,714,811 + Psest_3537 0.50 +2.1 3,715,107 - Psest_3537 0.84 +2.7 3,715,138 + Psest_3537 0.87 -1.3 3,715,138 + Psest_3537 0.87 -2.2 3,715,138 + Psest_3537 0.87 +3.1 3,715,139 - Psest_3537 0.87 -2.2 3,715,240 + -2.9 3,715,240 + +1.9 3,715,240 + -3.0 3,715,240 + +0.1 3,715,241 - +0.9 3,715,241 - +0.1 3,715,299 + -0.5 3,715,328 + -2.9 3,715,328 + +0.9 3,715,328 + +2.7 3,715,328 + -0.6 3,715,328 + -1.4 3,715,329 - +0.2 3,715,329 - -2.1 3,715,385 + Psest_3538 0.14 +1.0 3,715,470 - Psest_3538 0.23 -1.7 3,715,470 - Psest_3538 0.23 +1.3 3,715,470 - Psest_3538 0.23 +1.1 3,715,756 + Psest_3538 0.50 -1.1 3,715,757 - Psest_3538 0.50 -3.2 3,715,757 - Psest_3538 0.50 -3.2 3,715,780 + Psest_3538 0.52 +1.5 3,715,780 + Psest_3538 0.52 -3.0 3,715,780 + Psest_3538 0.52 -2.1 3,715,780 + Psest_3538 0.52 -2.5 3,715,781 - Psest_3538 0.52 -1.1 3,715,781 - Psest_3538 0.52 -1.2 3,715,781 - Psest_3538 0.52 +2.5 3,715,781 - Psest_3538 0.52 -2.5 3,715,781 - Psest_3538 0.52 +0.5 3,715,868 + Psest_3538 0.60 -4.4 3,715,868 + Psest_3538 0.60 +0.3 3,715,868 + Psest_3538 0.60 -3.0 3,715,868 + Psest_3538 0.60 -0.1 3,715,868 + Psest_3538 0.60 -0.7 3,715,869 - Psest_3538 0.61 +1.3 3,716,130 - Psest_3538 0.85 -2.1 3,716,179 + +1.2 3,716,179 + -4.6 3,716,180 - -0.5 3,716,180 - +1.9 3,716,180 - +1.0 3,716,215 + -1.2 3,716,215 + -0.7 3,716,215 + -0.8 3,716,215 + -0.5 3,716,215 + +0.0 3,716,216 - +0.8 3,716,216 - +0.2 3,716,216 - +1.5 3,716,216 - +2.2 3,716,216 - +0.4 3,716,216 - -0.1 3,716,216 - +1.8 3,716,216 - -4.0 3,716,216 - +0.1 3,716,216 - -1.4 3,716,216 - +1.5 3,716,620 - Psest_3539 0.26 -1.7 3,716,764 + Psest_3539 0.38 -1.8 3,716,764 + Psest_3539 0.38 -2.8 3,716,764 + Psest_3539 0.38 -3.8 3,716,764 + Psest_3539 0.38 -4.1 3,716,764 + Psest_3539 0.38 +3.4 3,716,764 + Psest_3539 0.38 +1.6 3,716,765 - Psest_3539 0.38 -0.7 3,716,765 - Psest_3539 0.38 -0.5 3,716,765 - Psest_3539 0.38 -1.4 3,716,765 - Psest_3539 0.38 -1.9 3,716,765 - Psest_3539 0.38 -1.7 3,716,765 - Psest_3539 0.38 +2.3 3,716,765 - Psest_3539 0.38 -3.0 3,716,765 - Psest_3539 0.38 -0.5 3,716,765 - Psest_3539 0.38 -0.2 3,716,765 - Psest_3539 0.38 +1.1 3,716,765 - Psest_3539 0.38 -1.8 3,716,959 + Psest_3539 0.54 -3.0 3,716,960 - Psest_3539 0.54 +0.8 3,716,960 - Psest_3539 0.54 +1.2 3,716,960 - Psest_3539 0.54 -0.1 3,717,245 + Psest_3539 0.77 +0.3 3,717,246 - Psest_3539 0.77 +1.6
Or see this region's nucleotide sequence