Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0336 and Psest_0337 are separated by 92 nucleotides Psest_0337 and Psest_0338 are separated by 219 nucleotides Psest_0338 and Psest_0339 are separated by 163 nucleotides Psest_0339 and Psest_0340 are separated by 63 nucleotides Psest_0340 and Psest_0341 are separated by 20 nucleotides
Psest_0336: Psest_0336 - Protein of unknown function (DUF3509)., at 358,734 to 359,012
_0336
Psest_0337: Psest_0337 - Regulator of cell morphogenesis and NO signaling, at 359,105 to 359,578
_0337
Psest_0338: Psest_0338 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 359,798 to 362,008
_0338
Psest_0339: Psest_0339 - hypothetical protein, at 362,172 to 362,501
_0339
Psest_0340: Psest_0340 - hypothetical protein, at 362,565 to 362,747
_0340
Psest_0341: Psest_0341 - flavoprotein, HI0933 family, at 362,768 to 363,952
_0341
Position (kb) 359
360
361
362
363 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 358.994 kb on + strand at 358.995 kb on - strand at 358.995 kb on - strand at 358.998 kb on + strand at 358.998 kb on + strand at 358.998 kb on + strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 359.005 kb on - strand at 359.201 kb on - strand, within Psest_0337 at 359.237 kb on - strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.277 kb on - strand, within Psest_0337 at 359.277 kb on - strand, within Psest_0337 at 359.312 kb on + strand, within Psest_0337 at 359.312 kb on + strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.602 kb on - strand at 359.602 kb on - strand at 359.602 kb on - strand at 359.603 kb on + strand at 359.745 kb on + strand at 359.745 kb on + strand at 359.745 kb on + strand at 359.746 kb on - strand at 359.746 kb on - strand at 359.766 kb on + strand at 359.766 kb on + strand at 359.767 kb on - strand at 359.871 kb on - strand at 359.961 kb on - strand at 360.047 kb on + strand, within Psest_0338 at 360.047 kb on + strand, within Psest_0338 at 360.047 kb on + strand, within Psest_0338 at 360.048 kb on - strand, within Psest_0338 at 360.048 kb on - strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.149 kb on + strand, within Psest_0338 at 360.149 kb on + strand, within Psest_0338 at 360.170 kb on + strand, within Psest_0338 at 360.170 kb on + strand, within Psest_0338 at 360.224 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.279 kb on - strand, within Psest_0338 at 360.279 kb on - strand, within Psest_0338 at 360.355 kb on + strand, within Psest_0338 at 360.548 kb on + strand, within Psest_0338 at 360.549 kb on - strand, within Psest_0338 at 360.575 kb on + strand, within Psest_0338 at 360.575 kb on + strand, within Psest_0338 at 360.575 kb on + strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.774 kb on - strand, within Psest_0338 at 361.262 kb on + strand, within Psest_0338 at 361.299 kb on - strand, within Psest_0338 at 361.475 kb on + strand, within Psest_0338 at 361.475 kb on + strand, within Psest_0338 at 361.476 kb on - strand, within Psest_0338 at 361.476 kb on - strand, within Psest_0338 at 361.706 kb on + strand, within Psest_0338 at 361.984 kb on - strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.581 kb on + strand at 362.581 kb on + strand at 362.582 kb on - strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.866 kb on - strand at 362.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 358,994 + +1.2 358,995 - -0.2 358,995 - -0.3 358,998 + -1.9 358,998 + +0.4 358,998 + -1.0 358,999 - -0.9 358,999 - -1.9 358,999 - +0.6 358,999 - -1.0 358,999 - -0.2 358,999 - +1.6 358,999 - -0.3 359,005 - -2.0 359,201 - Psest_0337 0.20 +1.3 359,237 - Psest_0337 0.28 +1.2 359,276 + Psest_0337 0.36 -1.0 359,276 + Psest_0337 0.36 +0.9 359,276 + Psest_0337 0.36 -0.8 359,276 + Psest_0337 0.36 -2.3 359,276 + Psest_0337 0.36 +0.2 359,276 + Psest_0337 0.36 +2.8 359,277 - Psest_0337 0.36 -0.3 359,277 - Psest_0337 0.36 +0.7 359,312 + Psest_0337 0.44 +0.8 359,312 + Psest_0337 0.44 -0.3 359,313 - Psest_0337 0.44 +1.1 359,313 - Psest_0337 0.44 -0.3 359,313 - Psest_0337 0.44 +0.3 359,313 - Psest_0337 0.44 +1.4 359,602 - +0.0 359,602 - -1.0 359,602 - +0.3 359,603 + +1.2 359,745 + -3.4 359,745 + -2.2 359,745 + -1.7 359,746 - -2.8 359,746 - -1.3 359,766 + -1.3 359,766 + +1.8 359,767 - +0.9 359,871 - -0.1 359,961 - +2.0 360,047 + Psest_0338 0.11 +1.0 360,047 + Psest_0338 0.11 -1.5 360,047 + Psest_0338 0.11 +1.3 360,048 - Psest_0338 0.11 -0.7 360,048 - Psest_0338 0.11 -1.7 360,086 + Psest_0338 0.13 -0.8 360,086 + Psest_0338 0.13 +0.6 360,086 + Psest_0338 0.13 -1.7 360,086 + Psest_0338 0.13 -1.0 360,086 + Psest_0338 0.13 +1.3 360,086 + Psest_0338 0.13 -2.3 360,086 + Psest_0338 0.13 -2.5 360,086 + Psest_0338 0.13 -0.0 360,086 + Psest_0338 0.13 +0.0 360,086 + Psest_0338 0.13 +0.9 360,087 - Psest_0338 0.13 +0.1 360,087 - Psest_0338 0.13 +2.5 360,087 - Psest_0338 0.13 -0.3 360,087 - Psest_0338 0.13 -1.5 360,087 - Psest_0338 0.13 +0.5 360,087 - Psest_0338 0.13 -0.6 360,087 - Psest_0338 0.13 +0.2 360,087 - Psest_0338 0.13 -4.3 360,149 + Psest_0338 0.16 +1.5 360,149 + Psest_0338 0.16 -1.1 360,170 + Psest_0338 0.17 -0.7 360,170 + Psest_0338 0.17 -0.8 360,224 + Psest_0338 0.19 +0.3 360,278 + Psest_0338 0.22 +0.8 360,278 + Psest_0338 0.22 +0.2 360,278 + Psest_0338 0.22 -0.6 360,278 + Psest_0338 0.22 -2.3 360,279 - Psest_0338 0.22 -0.4 360,279 - Psest_0338 0.22 -3.0 360,355 + Psest_0338 0.25 -2.6 360,548 + Psest_0338 0.34 -2.1 360,549 - Psest_0338 0.34 -2.7 360,575 + Psest_0338 0.35 -2.1 360,575 + Psest_0338 0.35 -2.7 360,575 + Psest_0338 0.35 +1.1 360,576 - Psest_0338 0.35 -1.3 360,576 - Psest_0338 0.35 -1.8 360,576 - Psest_0338 0.35 -0.3 360,576 - Psest_0338 0.35 +2.8 360,576 - Psest_0338 0.35 -1.1 360,576 - Psest_0338 0.35 -2.9 360,774 - Psest_0338 0.44 +1.9 361,262 + Psest_0338 0.66 -1.8 361,299 - Psest_0338 0.68 +0.4 361,475 + Psest_0338 0.76 +1.0 361,475 + Psest_0338 0.76 +2.9 361,476 - Psest_0338 0.76 +0.9 361,476 - Psest_0338 0.76 +0.1 361,706 + Psest_0338 0.86 +0.0 361,984 - +1.8 362,180 + +0.0 362,180 + -1.1 362,180 + +3.2 362,180 + +1.3 362,180 + -0.9 362,180 + -1.5 362,180 + -1.8 362,180 + -0.3 362,180 + +5.9 362,181 - +0.4 362,181 - +4.7 362,181 - +0.9 362,181 - +0.8 362,181 - -0.3 362,181 - -0.8 362,184 + +1.0 362,184 + +2.6 362,184 + +0.0 362,184 + -2.6 362,184 + +2.0 362,185 - +1.0 362,185 - +0.4 362,185 - +1.5 362,185 - -2.2 362,577 + +1.7 362,577 + +1.0 362,577 + +0.8 362,577 + -1.2 362,578 - +1.9 362,578 - -2.7 362,578 - -0.5 362,578 - -2.4 362,578 - +2.3 362,578 - +3.0 362,578 - -1.2 362,578 - +0.4 362,578 - +0.1 362,581 + +3.6 362,581 + +0.7 362,582 - -1.7 362,775 + -0.8 362,775 + +0.5 362,775 + +2.0 362,775 + +2.3 362,775 + -2.2 362,775 + +1.3 362,776 - -1.0 362,776 - +2.1 362,776 - +0.4 362,776 - +0.9 362,776 - -0.4 362,776 - +1.1 362,865 + -2.3 362,865 + +1.1 362,865 + -1.0 362,865 + -2.1 362,865 + -0.6 362,865 + +0.2 362,866 - -2.0 362,866 - -2.1
Or see this region's nucleotide sequence