Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0259

Experiment: RCH2 grown on a colony of N2F3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0258 and Psest_0259 overlap by 4 nucleotidesPsest_0259 and Psest_0260 overlap by 4 nucleotides Psest_0258: Psest_0258 - signal recognition particle-docking protein FtsY, at 272,579 to 273,859 _0258 Psest_0259: Psest_0259 - cell division ATP-binding protein FtsE, at 273,856 to 274,527 _0259 Psest_0260: Psest_0260 - Cell division protein, at 274,524 to 275,543 _0260 Position (kb) 273 274 275Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 274.314 kb on - strand, within Psest_0259at 274.347 kb on + strand, within Psest_0259at 275.283 kb on + strand, within Psest_0260at 275.284 kb on - strand, within Psest_0260at 275.304 kb on + strand, within Psest_0260at 275.305 kb on - strand, within Psest_0260at 275.491 kb on + strandat 275.491 kb on + strandat 275.492 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3
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274,314 - Psest_0259 0.68 +2.9
274,347 + Psest_0259 0.73 -0.9
275,283 + Psest_0260 0.74 -1.1
275,284 - Psest_0260 0.75 +0.9
275,304 + Psest_0260 0.76 -2.0
275,305 - Psest_0260 0.77 -0.3
275,491 + -0.8
275,491 + -0.6
275,492 - -3.3

Or see this region's nucleotide sequence