Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0236 and Psest_0237 are separated by 102 nucleotides Psest_0237 and Psest_0238 are separated by 129 nucleotides Psest_0238 and Psest_0239 are separated by 56 nucleotides Psest_0239 and Psest_0240 are separated by 115 nucleotides
Psest_0236: Psest_0236 - Predicted SAM-dependent methyltransferases, at 246,797 to 247,993
_0236
Psest_0237: Psest_0237 - intracellular protease, PfpI family, at 248,096 to 248,635
_0237
Psest_0238: Psest_0238 - Predicted signal transduction protein, at 248,765 to 249,631
_0238
Psest_0239: Psest_0239 - formamidopyrimidine-DNA glycosylase (fpg), at 249,688 to 250,500
_0239
Psest_0240: Psest_0240 - hypothetical protein, at 250,616 to 250,957
_0240
Position (kb)
248
249
250 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 247.924 kb on - strand at 247.924 kb on - strand at 247.924 kb on - strand at 248.026 kb on - strand at 248.419 kb on - strand, within Psest_0237 at 248.453 kb on + strand, within Psest_0237 at 248.454 kb on - strand, within Psest_0237 at 248.600 kb on + strand at 248.601 kb on - strand at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.176 kb on + strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.177 kb on - strand, within Psest_0238 at 249.196 kb on + strand, within Psest_0238 at 249.197 kb on - strand, within Psest_0238 at 249.260 kb on + strand, within Psest_0238 at 249.261 kb on - strand, within Psest_0238 at 249.261 kb on - strand, within Psest_0238 at 249.338 kb on + strand, within Psest_0238 at 249.338 kb on + strand, within Psest_0238 at 249.339 kb on - strand, within Psest_0238 at 249.339 kb on - strand, within Psest_0238 at 249.339 kb on - strand, within Psest_0238 at 249.563 kb on + strand at 249.563 kb on + strand at 249.563 kb on + strand at 249.564 kb on - strand at 249.564 kb on - strand at 249.581 kb on + strand at 249.581 kb on + strand at 249.674 kb on - strand at 249.862 kb on + strand, within Psest_0239 at 249.909 kb on + strand, within Psest_0239 at 250.004 kb on - strand, within Psest_0239 at 250.197 kb on + strand, within Psest_0239 at 250.197 kb on + strand, within Psest_0239 at 250.198 kb on - strand, within Psest_0239 at 250.198 kb on - strand, within Psest_0239 at 250.198 kb on - strand, within Psest_0239 at 250.198 kb on - strand, within Psest_0239 at 250.198 kb on - strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.201 kb on + strand, within Psest_0239 at 250.202 kb on - strand, within Psest_0239 at 250.202 kb on - strand, within Psest_0239 at 250.202 kb on - strand, within Psest_0239 at 250.202 kb on - strand, within Psest_0239 at 250.202 kb on - strand, within Psest_0239 at 250.202 kb on - strand, within Psest_0239 at 250.202 kb on - strand, within Psest_0239 at 250.477 kb on + strand at 250.560 kb on + strand at 250.560 kb on + strand at 250.560 kb on + strand at 250.560 kb on + strand at 250.560 kb on + strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.561 kb on - strand at 250.562 kb on + strand at 250.563 kb on - strand at 250.563 kb on - strand at 250.563 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 247,924 - -0.4 247,924 - -3.0 247,924 - +0.8 248,026 - +4.0 248,419 - Psest_0237 0.60 -2.0 248,453 + Psest_0237 0.66 -0.7 248,454 - Psest_0237 0.66 -0.8 248,600 + +1.0 248,601 - -0.8 249,176 + Psest_0238 0.47 -1.7 249,176 + Psest_0238 0.47 -0.0 249,176 + Psest_0238 0.47 -1.9 249,176 + Psest_0238 0.47 -1.6 249,176 + Psest_0238 0.47 -0.7 249,176 + Psest_0238 0.47 -0.0 249,176 + Psest_0238 0.47 -0.4 249,176 + Psest_0238 0.47 -3.9 249,177 - Psest_0238 0.48 -2.9 249,177 - Psest_0238 0.48 +1.9 249,177 - Psest_0238 0.48 -0.9 249,177 - Psest_0238 0.48 +2.2 249,177 - Psest_0238 0.48 -2.5 249,177 - Psest_0238 0.48 +0.3 249,177 - Psest_0238 0.48 -0.0 249,177 - Psest_0238 0.48 -1.3 249,177 - Psest_0238 0.48 +2.0 249,177 - Psest_0238 0.48 -2.5 249,196 + Psest_0238 0.50 -3.4 249,197 - Psest_0238 0.50 -2.2 249,260 + Psest_0238 0.57 +2.0 249,261 - Psest_0238 0.57 +0.9 249,261 - Psest_0238 0.57 -0.6 249,338 + Psest_0238 0.66 +1.7 249,338 + Psest_0238 0.66 -3.1 249,339 - Psest_0238 0.66 -2.3 249,339 - Psest_0238 0.66 -2.9 249,339 - Psest_0238 0.66 -1.5 249,563 + +3.1 249,563 + -3.0 249,563 + -3.1 249,564 - +1.1 249,564 - -0.4 249,581 + +0.6 249,581 + -1.3 249,674 - +1.5 249,862 + Psest_0239 0.21 +5.5 249,909 + Psest_0239 0.27 +0.2 250,004 - Psest_0239 0.39 -0.5 250,197 + Psest_0239 0.63 +0.9 250,197 + Psest_0239 0.63 -2.5 250,198 - Psest_0239 0.63 -1.5 250,198 - Psest_0239 0.63 -0.3 250,198 - Psest_0239 0.63 -1.2 250,198 - Psest_0239 0.63 +2.5 250,198 - Psest_0239 0.63 -0.1 250,201 + Psest_0239 0.63 +2.1 250,201 + Psest_0239 0.63 +2.8 250,201 + Psest_0239 0.63 +0.9 250,201 + Psest_0239 0.63 +0.2 250,201 + Psest_0239 0.63 -3.9 250,201 + Psest_0239 0.63 -2.3 250,201 + Psest_0239 0.63 -0.3 250,201 + Psest_0239 0.63 -2.6 250,201 + Psest_0239 0.63 +0.0 250,201 + Psest_0239 0.63 -1.6 250,202 - Psest_0239 0.63 +0.4 250,202 - Psest_0239 0.63 -0.7 250,202 - Psest_0239 0.63 -0.6 250,202 - Psest_0239 0.63 -1.3 250,202 - Psest_0239 0.63 -1.7 250,202 - Psest_0239 0.63 +0.1 250,202 - Psest_0239 0.63 -1.5 250,477 + -1.5 250,560 + -2.5 250,560 + -3.1 250,560 + -0.4 250,560 + -1.9 250,560 + +2.4 250,561 - +1.5 250,561 - +3.2 250,561 - +0.1 250,561 - +0.8 250,561 - -0.4 250,561 - -0.4 250,561 - +3.3 250,561 - -0.3 250,561 - +3.1 250,561 - -2.5 250,561 - +2.7 250,561 - +0.6 250,562 + +0.8 250,563 - +1.9 250,563 - +1.7 250,563 - -2.4
Or see this region's nucleotide sequence